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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 7 (1968), S. 2826-2833 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 199 (1963), S. 1166-1168 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] ZONAL centrifugation has been widely used for virus purification using either rate or equilibrium methods1. A limiting factor has been the small capacity of the swinging-bucket rotors generally used. Using a recently developed ultracentrifuge2,3, I have purified large quantities of virus particles ...
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 172 (1953), S. 807-808 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The physical properties of purified deoxyribonucleic acid vary widely, depending on the preparative techniques employed2-4. Poor reproducibility of viscosity measurements has been obtained on duplicate preparations from the same material5. A number of investigators have shown that the viscosity of ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 221 (1969), S. 1255-1256 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Commercial production of viral antigens for vaccines involving large quantities of harvested material requires processing with a minimum number of steps. A single step process for purification and concentration should yield more potent and safer parental vaccines. The dose of current influenza ...
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 66 (1962), S. 1984-1989 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 230 (1974), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 227 (1970), S. 1346-1347 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] First, if virus infection served no useful function, evolution of effective means for its elimination might be expected. "Natural" resistance is often due to lack of specific virus attachment sites4. One may ask why these persist if their sole function is to assist in acquiring illness. Interferon ...
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Annals of biomedical engineering 2 (1974), S. 1-18 
    ISSN: 1573-9686
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Bioengineering has many significant contributions to make to improvement in the delivery of health care. However, when major research and development efforts are involved, great care must be exercised in project choice to avoid as far as possible needless expense. Even those programs which ultimately result in very significant benefits at low cost to the average citizen pass a high-cost barrier which may take years to cross. A major mistake is to make decisions on the basis of too short a time span. Nearly all biophysical measurements, many tests, and some drugs have all at one time been too costly for general use. Then as the value of the advance became appreciated, inexpensive means for achieving the same ends were devised. A number of bioengineering problems are reviewed which appear feasible but which are not now being done, largely for organizational reasons, to illustrate the point that many worthwhile projects exist now. The importance of decision making in bioengineering on future health-care quality and costs is apparent. Much of future work will probably be at the molecular level. Since support is now fragmented to the point where continued advancement is in jeopardy, it is important that problems in the support and organization of bioengineering and biomedical research be brought continually to public attention.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 261 (1972), S. 257-271 
    ISSN: 1618-2650
    Keywords: Analysatoren ; Entwicklung eines schnellen Analysators für physiologische Proben
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Im Oak Ridge National Laboratory wurde ein neues Analysatorsystem entwickelt, das für die Analyse physiologischer Proben bestimmt ist und in klinischen Laboratorien, Krankenhäusern, Unfallstationen, Forschungsinstituten sowie in einer verkleinerten Version auch in der Raumfahrt zum Einsatz gelangen kann. Das System zeichnet sich aus durch leichte Automatisierbarkeit, Flexibilität, rasche Inbetriebnahme und eignet sich für parallel durchgeführte Serienanalysen. Die folgenden Charakteristica des Systems werden diskutiert: 1. Dynamisches Mischen von Proben und Reagentien durch zentrifugale Übertragung in die Küvetten, 2. Entleeren, Spülen und Trocknen der Küvetten durch Rotation, 3. Genaue Temperaturkontrolle, 4. Spektrophotometrische Reaktionsüberwachung, 5. Datenberechnung mit Speicherung aller Werte für möglichen Abruf, 6. Feedback-Kontrolle zur Fehlerkorrektur und Zeiteinsparung. Mögliche zukünftige Verbesserungen werden erwähnt.
    Notes: Summary New instrumentation has been developed at ORNL for analyzing physiologic specimens. The system is designed for use in clinical laboratories, hospitals, and emergency rooms and for use by researchers. Miniaturized versions may also be used in prolonged space flights. After consideration of ease of automation, flexibility, and rapid startup, multiple analyses performed in parallel was chosen as the desired approach. Concepts employed in developing the system are discussed, including: (1) dynamic mixing of samples and reagents through centrifugal transfer from transfer disks into cuvets; (2) rotor design allowing emptying, flushing, and drying of cuvets; (3) precise temperature control; (4) spectrophotometric reaction monitoring; (5) data reduction, with all data stored for possible recall; (6) feedback control for error correction and time saving. Possible future improvements are indicated.
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  • 10
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Liver proteins of male C57BL/6T mice treated with 0, 50, or 250 mg/kg Aroclor 1254 were analyzed by high-resolution two-dimensional (2-D) electrophoresis. The resulting patterns were processed using a computerized image analysis system and quantitative data selected for a total of 150 protein spots. On the basis of an analysis of liver proteins form five animals in each treatment group, we found 31 proteins that showed quantitative differences attributable to treatment with chlorinated hydrocarbons at a high level of statistical significance. One of the altered proteins appears to be Mitcon:2, a heat-shock sensitive mitochondrial matrix polypeptide; another appears likely to be microsomal cytochrome b5. The results indicate that quantitative 2-D protein mapping may reveal much more detail regarding the in vivo effects of toxic xenobiotics than has previously been available, and thus allow a more informative approach to the testing of toxic compounds.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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