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  • 1
    Electronic Resource
    Electronic Resource
    Growth dynamics and cytoskeleton organization during stem maturation and gravity-induced stem bending in Zea mays L. (1998)
    Springer
    Planta 207 (1998), S. 246-258 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Actin ; Cell elongation ; Gravitropism ; Microtubule ; Pulvinus ; Zea (gravitropism)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Characterization of gravitropic bending in the maize stem pulvinus, a tissue that functions specifically in gravity responses, demonstrates that the pulvinus is an ideal system for studying gravitropism. Gravistimulation during the second of three developmental phases of the pulvinus induces a gradient of cell elongation across the non-growing cells of the pulvinus, with the most elongation occurring on the lower side. This cell elongation is spatially and temporally separated from normal internodal cell elongation. The three characterized growth phases in the pulvinus correspond closely to a specialized developmental sequence in which structural features typical of cells not fully matured are retained while cell maturation occurs in surrounding internodal and nodal tissue. For example, the lignification of supporting tissue and rearrangement of transverse microtubules to oblique that occur in the internode when cell elongation ceases are delayed for up to 10 d in the adjacent cells of the pulvinus, and only occurs as a pulvinus loses its capacity to respond to gravistimulation. Gravistimulation does not modify this developmental sequence. Neither wall lignification nor rearrangement of transverse microtubules occurs in the rapidly elongating lower side or non-responsive upper side of the pulvinus until the pulvinus loses the capacity to bend further. Gravistimulation does, however, lead to the formation of putative pit fields within the expanding cells of the pulvinus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050480
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  • 2
    Electronic Resource
    Electronic Resource
    Macrophages form circular zones of very close apposition to lgG-Coated surfaces (1990)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 15 (1990), S. 260-270 
    Details
    ISSN: 0886-1544
    Keywords: Fc-receptors ; antibodies ; “frustrated” phagocytosis ; leucocytes ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: When phagocytes spread on surfaces coated with ligands such as IgG, they form a tight seal with the substrate. This seal excludes soluble macromolecules in the medium from the interface between the cell and substrate. In contrast, when cells spread on control surfaces that are not coated with ligands, the underside of the cell remains freely accessible to soluble proteins (Wright and Silverstein: Nature 309:359, 1984). We employed reflection-interference microscopy (RIM) to determine where the seal forms during interaction with ligand (IgG) -coated surfaces. Human monocyte-derived macrophages (MO) were plated at 37°C on dinitrophenylated (DNP)-glass coverslips (control substrate), IgM anti-DNP-DNP-coated glass (control substrate), or on IgG anti-DNP-DNP-coated glass (phagocytosis-promoting substrate). Live or fixed cells were examined by RIM. Spreading on control surfaces at 37°C was complete in 25 minutes, whereas spreading on IgG-coated surfaces was maximal within 15 minutes and resulted in cell-substrate contact area 1.6 × that of control cells. Within 1 h at 37°C, 90% of MO that spread on IgG-coated substrates, but not on control substrates, excluded macromolecules from their underside. A minor population of cells (19%) exhibited a uniform iron gray RIM appearance indicating an even, close approach to the substrate. These cells may represent early stages of frustrated phagocytosis. In contrast to cells on control substrates, 70% of cells on IgG-coated substrates developed continuous peripheral dark rings in RIM indicative of close association with the substrate. Essentially all cells with peripheral dark rings in RIM excluded macromolecules from their underside. Enclosed within this ring was an area of greater separation between the cell membrane and the substrate, as indicated by the lighter grey of this region in RIM and by the accessibility of substrate to anti-substrate antibody when breaks in the dark ring occur. Thus, MO can create a closed compartment between plasma membrane and substrate that excludes proteins in the surrounding medium, thereby protecting substances secreted into this space from potentially inhibitory substances in the medium.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970150408
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  • 3
    Electronic Resource
    Electronic Resource
    βVLDL uptake by pigeon monocyte-derived macrophages: Correlation of binding dynamics with three-dimensional ultrastructure (1991)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 19 (1991), S. 139-151 
    Details
    ISSN: 0886-1544
    Keywords: lipoprotein ; receptor-mediated endocytosis ; nonspecific endocytosis ; microvilli ; membrane ruffles ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Endocytosis of pigeon beta migrating very-low-density lipoprotein (βVLDL) by monocyte-derived macrophages (monocyte/macrophages), cultured from Random Bred White Carneu (RBWC) pigeons, occurs by both coated and non-coated regions of the plasma membrane (Henson et al.: Exp. Mol. Pathol. 51:243-263, 1989). Secondary to binding, the βVLDL is translocated to lysosomes for degradation. Ultimately these events lead to foam cell formation in vitro. Utilizing video-enhanced contrast light microscopy in conjunction with whole mount intermediate-voltage transmission electron microscopy (IVEM) and high-resolution scanning EM, the dynamics of βVLDL binding have been correlated with ultrastructure. Beta VLDL conjugated to gold colloids was visualized at the surface of living cells by using Allen video-enhanced contrast-differential interference contrast microscopy (AVEC-DIC). Subsequent to AVEC-DIC, direct observation of the identical cells by IVEM and SEM was facilitated through the use of gold finder grids, and these EM observations confirmed identification of the videoobserved βVLDL particles.Upon addition of βVLDL, pigeon monocyte/macrophages underwent gross morphological changes. These changes were recorded by video as movements at the cytoplasmic periphery, and the movements involved extension of microvilli, expression of retraction fibers, and elaboration of membrane ruffles. When secondarily observed by stereo (3-D) IVEM and SEM, the identification of microvilli, retraction fibers, and membrane ruffles was confirmed and the lipoprotein-gold conjugates were associated with these ligand-induced membrane structures. Beta VLDL-gold conjugates were also associated with pit-like regions at the base of microvilli, while at the base of ruffles, βVLDL-gold conjugates were located in membrane invaginations and cytoplasmic vesicles.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970190302
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  • 4
    Electronic Resource
    Electronic Resource
    A pharmacological study of calcium flux mechanisms in the tannin vacuoles ofMimosa pudica L. motor cells (1993)
    Springer
    Protoplasma 176 (1993), S. 91-99 
    Details
    ISSN: 1615-6102
    Keywords: Thigmonasty ; Primary pulvinus ; Calcium pumps ; Calcium channels ; Antagonist ; Agonists
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When treated with various calcium-active agents, the volume changes in the tannin vacuoles of the abaxial (lower) motor cells in the sensitiveMimosa pudica L. correspond to the movement of the primary pulvinus. The calcium pump inhibitors, sodium metavanadate and erythrosin B, significantly inhibited the rate of recovery in both the in vivo and in vitro examinations, while the calcium channel blocker, verapamil, inhibited the closing response. Bay-K 8644 enhanced the in vivo effect and induced the in vitro effect, therefore appearing to be an agonist of the voltage regulated calcium channels in the tannin vacuoles. These observations support the hypothesis that calcium channels in the tannin vacuole membrane underly the thigmonastic response and pumps in the tannin vacuole membrane are involved in the mechanisms underlying recovery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01378944
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  • 5
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    Use of digital image analysis, viability stains, and germination assays to estimate conventional and glyphosate-resistant cotton pollen viability (2002)
    Wiley
    Details
    Publication Date: 2002-11-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 6
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    Growth dynamics and cytoskeleton organization during stem maturation and gravity-induced stem bending in Zea mays L. (1998)
    Springer
    Details
    Publication Date: 1998-11-20
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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  • 7
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    Demonstration of prominent actin filaments in the root columella (2001)
    Springer
    Details
    Publication Date: 2001-02-19
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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