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  • 1
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Giant axonal neuropathy (GAN) is a devastating sensory and motor neuropathy caused by mutations in the GAN gene, which encodes the ubiquitously expressed protein gigaxonin. Cytopathological features of GAN include axonal degeneration, with accumulation and aggregation ...
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  • 2
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The following two articles are concerned with the genetic control of mutation rate. The first describes the anti-mutagenic effect of some DNA polymerase mutations on phage T4 mutation rates, and discusses the implications they hold for the mechanism of action of DNA polymerase. The second compares ...
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  • 3
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Effects of ploughing or direct drilling with three methods of straw disposal on amounts of inoculum of Pyrenophora teres, and on frequency of infection and severity of net blotch in the autumn, were studied in winter barley. Prior to ploughing, many conidia of P. teres were caught above areas where infected straw from a previous crop of winter barley had been bated and removed leaving culm bases, or where barley straw had been chopped and left in situ, but relatively few were caught above areas where straw had been burnt. Thereafter, where ploughing had buried surface residues, irrespective of the method of straw disposal, conidia were not caught for at least 3 weeks, and subsequently were substantially fewer than in direct-drilled areas where many spores were caught. Production of conidia (measured as numbers per unit length of straw) was greatest on chopped straw, less on culm bases and least on burnt straw residues. Sporulation on volunteer barley plants was much reduced by application of paraquat + diquat, but some still occurred on visually‘dead’volunteer barley.All direct-drilled barley plants were diseased within 27 days of sowing, whereas 42 days elapsed before all plants sown in ploughed areas were diseased. Disease on individual plants was also more severe in direct-drilled areas: 20% of the area of the first leaf to emerge was diseased 19 days after crop emergence in direct-drilled plots, whereas less than 9% was diseased in ploughed areas 50 days after emergence.There was an additive effect of straw disposal methods and direct-drilling on disease, which in turn affected plant vigour. The adverse effect of direct-drilling on the incidence and severity of net blotch appeared to be far greater than that of the straw disposal methods.
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 289 (1981), S. 405-407 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We are interested in BCG because it can be used to induce CGI in the lungs of rabbits1 and in the lungs and spleens of only certain strains of inbred mice2. A seemingly paradoxical observation has been that inbred mice which develop CGI in response to BCG are suppressed in their response to ...
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  • 5
    ISSN: 1432-1009
    Keywords: Tidal salt marsh restoration ; Macroinvertebrates ; Melampus bidentatus ; Geukensia demissa ; Reference marshes ; Sampling methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Macroinvertebrates were examined on an impounded valley marsh in Stonington, Connecticut, that has changed from aTypha-dominated system to one with typical salt-marsh vegetation during 13 years following the reintroduction of tidal exchange. Animal populations on this restored impounded marsh were evaluated by comparing them with populations on a nearby unimpounded valley marsh of roughly the same size. Populations of the high marsh snail,Melampus bidentatus Say, were quantitatively sampled along transects that extended from the water-marsh edge to the upland; those of the ribbed mussel,Geukensia demissa Dillwyn, were sampled in low marsh areas on transects along the banks of creeks and mosquito ditches. The occurrence of other marsh invertebrates also was documented, but their abundance was not measured. The mean density ofMelampus was 332±39.6 SE/m2 on the restored impounded marsh and 712±56.0 SE/m2 on the unimpounded marsh. However, since snails were larger on the restored impounded marsh, the difference in snail biomass was less pronounced than the difference in snail density. MeanMelampus biomass was 4.96±0.52 SE g dry wt/m2 on the restored impounded marsh and 6.96±0.52 SE g dry wt/m2 on the unimpounded marsh. On the two marshes, snail density and biomass varied in relation to plant cover and other factors. The density and biomass ofGeukensia at the edge of the marsh were comparable on the restored impounded and unimpounded marshes. Mean mussel densities ranged from 80 to 240/m2 and mean mussel biomass varied from 24.8–64.8 g dry wt/m2 in different low marsh areas. In contrast, below the impoundment dike, meanGeukensia density was 1100±96.4 SE/m2 and meanGeukensia biomass was 303.6±33.28 SE g dry wt/m2. A consideration of all available evidence leads to the conclusion that the impounded marsh is in an advanced phase of restoration.
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  • 6
    ISSN: 1573-4927
    Keywords: thioguanine ; Lesch-Nyhan ; heterozygote detection ; mosaicism ; lymphocyte ; somatic cell genetics ; mutation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In females heterozygous for the Lesch-Nyhan (LN) mutation, there is mosaicism of peripheral blood lymphocytes (PBLs) with regard to sensitivity to 6-thioguanine (TG) inhibition of tritiated thymidine ([3H]Tdr) incorporation following phytohemagglutinin (PHA) stimulation. That there are two populations of PBLs, normal and mutant (LN-like), has been demonstrated by an autoradiographic enumerative assay. A single three-generation family containing six potentially heterozygous females was studied. Five of the six were mosaics with frequencies of TG-resistant (TGr) PBLs ranging from 1.4×10−3 to 4.2×10−3 when tested at 2×10−4 m TG. The median frequency of TGr PBLs in 63 healthy non-LN individuals between the ages of 11 and 75 years was found to be 1.1×10−4 (mean 1.3×10−4) (10th and 90th percentiles—6.1×10−5 and 2.1×10−4) and was not age related. The sixth potentially heterozygous female in the current family had a TGr PBL frequency of 1.9×10−4. In the five females with elevated TGr PBL frequencies, TGr skin fibroblasts with frequencies ranging from 26% to 100% of the sample tested were found; in the female with the normal TGr PBL frequency, no TGr skin fibroblasts were found. The former group was considered to be LN heterozygous. Four of the five had been previously diagnosed as such. The latter individual is considered to be genotypically normal. Females who are heterozygotes for the LN mutation have two populations of PBLs.
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  • 7
    Publication Date: 2018-06-11
    Description: Environmental changes threaten agricultural production, food security, and health. Previous reviews suggest that environmental changes will substantially affect future yields of starchy dietary staples. To date, no comprehensive global analysis of the impacts of environmental change on (nonstaple) vegetables and legumes—important constituents of healthy diets—has been reported. We systematically searched for articles published between 1975 and 2016 on the effects of ambient temperature, tropospheric carbon dioxide (CO2), and ozone (O3) concentrations, water availability, and salinization on yields and nutritional quality of vegetables and legumes. We estimated mean effects of standardized environmental changes using observed exposure-response relationships and conducted meta-analyses where possible. We identified 174 relevant papers reporting 1,540 experiments. The mean (95% CI) reported yield changes for all vegetables and legumes combined were +22.0% (+11.6% to +32.5%) for a 250-ppm increase in CO2concentration, −8.9% (−15.6% to −2.2%) for a 25% increase in O3concentration,−34.7% (−44.6% to −24.9%) for a 50% reduction in water availability, and −2.3% (−3.7% to −0.9%) for a 25% increase in salinity. In papers with baseline temperatures 〉20 °C, a 4 °C increase in temperature reduced mean yields by −31.5% (−41.4% to −21.5%). Impacts of environmental changes on nutritional quality were mixed. In a business-as-usual scenario, predicted changes in environmental exposures would lead to reductions in yields of nonstaple vegetables and legumes. Where adaptation possibilities are limited, this may substantially change their global availability, affordability, and consumption in the mid to long term. Our results stress the importance of prioritizing agricultural developments, to minimize potential reductions in vegetable and legume yields and associated negative health effects.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 8
    Publication Date: 2016-05-16
    Description: Staphylococcus aureusis a major bacterial pathogen, which causes severe blood and tissue infections that frequently emerge by autoinfection with asymptomatically carried nose and skin populations. However, recent studies report that bloodstream isolates differ systematically from those found in the nose and skin, exhibiting reduced toxicity toward leukocytes. In two patients, an attenuated toxicity bloodstream infection evolved from an asymptomatically carried high-toxicity nasal strain by loss-of-function mutations in the gene encoding the transcription factor repressor of surface proteins (rsp). Here, we report thatrspknockout mutants lead to global transcriptional and proteomic reprofiling, and they exhibit the greatest signal in a genome-wide screen for genes influencingS. aureussurvival in human cells. This effect is likely to be mediated in part viaSSR42, a long-noncoding RNA. We show thatrspcontrolsSSR42 expression, is induced by hydrogen peroxide, and is required for normal cytotoxicity and hemolytic activity. Rsp inactivation in laboratory- and bacteremia-derived mutants attenuates toxin production, but up-regulates other immune subversion proteins and reduces lethality during experimental infection. Crucially, inactivation ofrsppreserves bacterial dissemination, because it affects neither formation of deep abscesses in mice nor survival in human blood. Thus, we have identified a spontaneously evolving, attenuated-cytotoxicity, nonhemolyticS. aureusphenotype, controlled by a pleiotropic transcriptional regulator/noncoding RNA virulence regulatory system, capable of causingS. aureusbloodstream infections. Such a phenotype could promote deep infection with limited early clinical manifestations, raising concerns that bacterial evolution within the human body may contribute to severe infection.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 9
    Publication Date: 2012-11-01
    Print ISSN: 0002-1962
    Electronic ISSN: 1435-0645
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 10
    Publication Date: 2019
    Description: Chondrichthyes (sharks, rays, skates and chimaeras) are among the oldest extant predators and are vital to top-down regulation of oceanic ecosystems. They are an ecologically diverse group occupying a wide range of habitats and are thus, exploited by coastal, pelagic and deep-water fishing industries. Chondrichthyes are among the most data deficient vertebrate species groups making design and implementation of regulatory and conservation measures challenging. High-throughput sequencing technologies have significantly propelled ecological investigations and understanding of marine and terrestrial species’ populations, but there remains a paucity of NGS based research on chondrichthyan populations. We present a brief review of current methods to access genomic and metagenomic data from Chondrichthyes and discuss applications of these datasets to increase our understanding of chondrichthyan taxonomy, evolution, ecology and population structures. Last, we consider opportunities and challenges offered by genomic studies for conservation and management of chondrichthyan populations.
    Electronic ISSN: 1424-2818
    Topics: Biology
    Published by MDPI
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