ISSN:
0006-3592
Keywords:
5′-nucleotide
;
ester
;
alcoholysis
;
esterification
;
nucleotide pyrophosphatase
;
phosphodiesterase
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Nucleotide alkyl esters are pharmacologically important as potential (ant)agonists of purinoceptors and inhibitors of enzymes. Potato nucleotide pyrophosphatase (PNP) was compared with snake venom phosphodiesterase (SVP) as a catalyst to synthesize nucleotide alkyl esters. In methanol-water mixtures, the methanolysis/hydrolysis ratio of PNP, but not SVP, changed with pH and temperature, being optimal at high pH and low temperature. In a semi-preparative experiment, a crude PNP preparation produced 0.17 mM AMP-O-methyl ester (AMP-OMe) from 1 mM diadenosine 5′,5‴-P1,P2-diphosphate (AppA) and 5M methanol, at pH 9 and 0°C. Drawbacks to large-scale use are: low rates inherent to low temperatures, ATP unsuitability as a substrate for alcoholysis, and high cost of AppA. Advantages of PNP vs. SVP are cheapness, non-toxicity, and availability of the enzyme source. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:62-67, 1998.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
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