ALBERT

Description: Although studies showed that the most Phytoplankton blooms usually provide positive impacts on marine environments, but blooming of certain species of phytoplankton may also have negative impacts. In recent years there has been a growing concern of the problems associated with red tides, which may induce mass mortalities of cultured fish or shellfish, and cause damage to aquaculture industries such as prawn culture and caged fish culture. Therefore, mass blooming of harmful algae in the Persian Gulf and Oman Sea may cause unexpected damage to fish stocks (through the production of toxins, oxygen, feeding on fish eggs and primary producers) and ecosystem aquatic and fishing communities are faced with a serious problem. The aim of the present study initially was to Isolation and determine the parameters affecting the growth and booms harmful algal, determine the growth and survival of vannamei shrimp Post larve in different densities of the algae, as well as the best, safest and most effective ways to control the blooming of this group of phytoplankton (physico-chemical), respectively. In order to provide optimum growth and bloom forming according to characteristics of each species, phytoplankton was sampled during the bloom conditions in the coastal waters of Bandar Abbass, Hengam and Qeshm Islands with cleaned sampling bottles from May 2012 to June 2015. The samples were then filtered through phytoplankton net (100 µm) and poured into bottles containing 1 liter seawater and were sent to the Persian Gulf and Oman Sea Ecology Research Laboratory under cool conditions. After adaptation in filtered seawater, some species were isolated by positive phototropism characteristic. They were grown in modified media culture at different salinities (30, 32 and 35ppt), temperatures (20, 23, 26 and 28ºC) and intensities (35, 70 and 90 µmol m-2 s -1 ). During this part of study, three species of dinoflagellates, Protoperedinium sp., Noctiluca scintillans and Cochlodinium polykrikoides were identified, but we could isolate only Cochlodinium polykrikoides species. The first dinoflagellate species was Noctiluca scintillans which only survived for 4 months during several replacement of culture media (F/4) under salinity of 32 ppt, temperature of 25ºC and 11h light: 13h dark photoperiod regime. The next dinoflagellate species which formed temporary blooms was Protoperedinium sp., but was not be isolated by conventional culture media. Finally the last dinoflagellate species which we could success to isolate was Cochlodinium polykrikoides. The results of Isolation and determine the parameters affecting the growth and booms harmful algal, clearly showed that the highest alga biomass and growth rate was obtained following culture under the A2 medium, 32ppt salinity, 26°C temperature, and under at a light intensity of 90 µmol m-2 s -1 provided by cool white fluorescent tubes. Maximum cell density of C. polykrikoides in a 5 liter Erlenmeyer reached to 1.6 × 106 cell L−1 including chains of 2-12 cells and sometimes 16 cells for twenty days. Based on the results from the present study, providing suitable media culture and physical condition, bloom forming of C. polykrikoides start from day 8 to 10 and will be continued until day 24 to 28. In the other hand, C. polykrikoides cells immediately crashed and destroyed. In order to investigate the best, safest and most effective way to control of harmful algae blooms, we used physical methods (slurry clay: 0.5, 1, 2, 4 and 10 gl-1 ). The results of the physical assay showed that the growth of C.polykricoides was strongly inhibited by using clay slurry in 4 or 10 gl-1 . The removal efficiency of C. polykricoiedes by clay was 99% after 24 hours. The results of the physical assay on Protoperidinium quinquecorne showed that the growth was strongly inhibited by using clay slurry in 4 or 10 gl-1 . The removal efficiency of Protoperidinium quinquecorne by using 4 gl-1 clay was 99% and 10 gl-1 was 98 after 24 hours. However, using clay could be considered as an effective strategy to control HAB’S in the Persian Gulf coastal waters. Finally, in order to survey the effects of various densities of harmful algae on growth and survival rate of post on white leg shrimp postlarve, the vanamei shrimp postlarvae were exposed to different densities of C. polykrikoides (20000, 600000, and 2000000 cell/l) for 6 weeks culturing period. The results clearly showed that there were no significant differences between growth and survival rates of shrimp postlarvae exposed to different densities of C. polykrikoides during the experimental period.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Black lip pearl oyster (pinctada magaritifera) is one of the most important species of Pearl maker in the world. Its reserves in the Persian Gulf waters is facing to a variety of causes, especially oil pollution from Persian Gulf war that cause genetic changes in pearl oyster. 65 broodstocks black lip pearl oyster mature with average length of the dorsal ventral (15 ± 2cm DVM) were collected from its typical natural Lavan Island in the months of June and July 2007 and were transferred to the research station and then were washed in cold rooms (20 ° C). The gonad indices were determined by microscope based on the gametogenez steps. After 2 days from collection time, the broodstocks were exposed to thermal shock in order of spawning stimulation. For the analysis of biochemical compounds, microalgae were sampled at logarithmic phase stage with three replications. Firstly, the samples were concentrated by centrifuges (3500g, 20 min). After washing with ammonium formate solution 0.5 molar, the samples were again centrifuged at 3500 g for 15 min. After spawning, D shape larvae form and then the larva ambo feeded from different micro-algae and lipid and the amount of growth and survival rates were studied. D shape larvae with 3 larvae per ml density and dorsal- abdominal length of 78.9 ± 0.7 μm were cultured in 5 liter Erlenmeyer flask containing 2 liters water. Whereas, samples at umbo stage with average dorsal-ventral length of 133 ± 3.1 μm were cultured in 15 liters plastic containers at 27- 29 ° C and salinity of 34 to 35 parts per thousand. The studied microalgae were Isochrysis aff galbana, Chaetoceros calcitrans and c.muelleri. The results of biochemical compounds showed that protein is the most component of all species with a maximum amount of 527.5 ± 2.1 mg g-1 in T. Iso microalgae. Also, T. Iso has a maximum amount of lipids, following by seek c. muelleri and c. calcitrans, respectively. Test results showed that feeding of larvae black lip pearl oyster in stage D shape with microalgae T. Iso alone has more growth rate than double or triple mixtures. However, diatoms have a high nutritional value for larval mussels in umbo state, and they are accounted as important components in a double and triple mixture of microalgae diet. The size of the larval D shape after 10 days showed a difference among treatments. The cultured larves that feeded from T Iso reached to maximum length of 111.4 ± 10 μm and the maximum survival rate of 57.7% was related to larves that feeded by fresh microalgae T. Iso. D.V.M for microalgae with microalgae or lipid nutrition showed no significant difference.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Black lip pearl oyster (pinctada magaritifera) is one of the most important species of Pearl maker in the world. Its reserves in the Persian Gulf waters is facing to a variety of causes, especially oil pollution from Persian Gulf war that cause genetic changes in pearl oyster. 65 broodstocks black lip pearl oyster mature with average length of the dorsal ventral (15 ± 2cm DVM) were collected from its typical natural Lavan Island in the months of June and July 2007 and were transferred to the research station and then were washed in cold rooms (20 ° C). The gonad indices were determined by microscope based on the gametogenez steps. After 2 days from collection time, the broodstocks were exposed to thermal shock in order of spawning stimulation. For the analysis of biochemical compounds, microalgae were sampled at logarithmic phase stage with three replications. Firstly, the samples were concentrated by centrifuges (3500g, 20 min). After washing with ammonium formate solution 0.5 molar, the samples were again centrifuged at 3500 g for 15 min. After spawning, D shape larvae form and then the larva ambo feeded from different micro-algae and lipid and the amount of growth and survival rates were studied. D shape larvae with 3 larvae per ml density and dorsal- abdominal length of 78.9 ± 0.7 μm were cultured in 5 liter Erlenmeyer flask containing 2 liters water. Whereas, samples at umbo stage with average dorsal-ventral length of 133 ± 3.1 μm were cultured in 15 liters plastic containers at 27- 29 ° C and salinity of 34 to 35 parts per thousand. The studied microalgae were Isochrysis aff galbana, Chaetoceros calcitrans and c.muelleri. The results of biochemical compounds showed that protein is the most component of all species with a maximum amount of 527.5 ± 2.1 mg g^-1 in T. Iso microalgae. Also, T. Iso has a maximum amount of lipids, following by seek c. muelleri and c. calcitrans, respectively. Test results showed that feeding of larvae black lip pearl oyster in stage D shape with microalgae T. Iso alone has more growth rate than double or triple mixtures. However, diatoms have a high nutritional value for larval mussels in umbo state, and they are accounted as important components in a double and triple mixture of microalgae diet. The size of the larval D shape after 10 days showed a difference among treatments. The cultured larves that feeded from T Iso reached to maximum length of 111.4 ± 10 μm and the maximum survival rate of 57.7% was related to larves that feeded by fresh microalgae T. Iso. D.V.M for microalgae with microalgae or lipid nutrition showed no significant difference.