Publication Date:
2001-04-09
Description:
HIF (hypoxia-inducible factor) is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability. In the presence of oxygen, HIF is targeted for destruction by an E3 ubiquitin ligase containing the von Hippel-Lindau tumor suppressor protein (pVHL). We found that human pVHL binds to a short HIF-derived peptide when a conserved proline residue at the core of this peptide is hydroxylated. Because proline hydroxylation requires molecular oxygen and Fe(2+), this protein modification may play a key role in mammalian oxygen sensing.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ivan, M -- Kondo, K -- Yang, H -- Kim, W -- Valiando, J -- Ohh, M -- Salic, A -- Asara, J M -- Lane, W S -- Kaelin , W G Jr -- New York, N.Y. -- Science. 2001 Apr 20;292(5516):464-8. Epub 2001 Apr 5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Dana-Farber Cancer Institute and Brigham and Women's Hospital, Howard Hughes Medical Institute, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11292862" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Basic Helix-Loop-Helix Transcription Factors
;
Cell Hypoxia
;
Cell Line
;
Cobalt/pharmacology
;
Deferoxamine/pharmacology
;
Humans
;
Hydroxylation
;
Hydroxyproline/*metabolism
;
*Ligases
;
Mass Spectrometry
;
Mice
;
Molecular Sequence Data
;
Oxygen/*physiology
;
Protein Structure, Tertiary
;
Proteins/*metabolism
;
Recombinant Fusion Proteins/metabolism
;
Trans-Activators/chemistry/genetics/*metabolism
;
Transcription Factors/*metabolism
;
Tumor Cells, Cultured
;
*Tumor Suppressor Proteins
;
*Ubiquitin-Protein Ligases
;
Ubiquitins/metabolism
;
Von Hippel-Lindau Tumor Suppressor Protein
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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