Publication Date:
2013-02-09
Description:
Although many metazoan enzymes that add or remove specific modifications on histone proteins are essential transcriptional regulators, the functional significance of posttranslational modifications on histone proteins is not well understood. Here, we show in Drosophila that a point mutation in lysine 27 of histone H3 (H3-K27) fails to repress transcription of genes that are normally repressed by Polycomb repressive complex 2 (PRC2), the methyltransferase that modifies H3-K27. Moreover, differentiated H3-K27 mutant cells show homeotic transformations like those seen in PRC2 mutant cells. Taken together, these analyses demonstrate that H3-K27 is the crucial physiological substrate that PRC2 modifies for Polycomb repression.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pengelly, Ana Raquel -- Copur, Omer -- Jackle, Herbert -- Herzig, Alf -- Muller, Jurg -- New York, N.Y. -- Science. 2013 Feb 8;339(6120):698-9. doi: 10.1126/science.1231382.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Chromatin and Chromosome Biology Research Group, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23393264" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Differentiation
;
Drosophila Proteins/chemistry/*genetics/*metabolism
;
Drosophila melanogaster/*genetics/growth & development/metabolism
;
*Gene Expression Regulation
;
Genes, Homeobox
;
Genes, Insect
;
Histones/chemistry/*genetics/*metabolism
;
Lysine
;
Mutant Proteins/chemistry/metabolism
;
Nucleosomes/metabolism
;
Phenotype
;
Point Mutation
;
Polycomb Repressive Complex 2/*metabolism
;
Protein Processing, Post-Translational
;
Transcription, Genetic
;
Transgenes
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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