Publication Date:
2012-04-28
Description:
Endogenous S-nitrosylation of proteins, a principal mechanism of cellular signaling in eukaryotes, has not been observed in microbes. We report that protein S-nitrosylation is an obligate concomitant of anaerobic respiration on nitrate in Escherichia coli. Endogenous S-nitrosylation during anaerobic respiration is controlled by the transcription factor OxyR, previously thought to operate only under aerobic conditions. Deletion of OxyR resulted in large increases in protein S-nitrosylation, and S-nitrosylation of OxyR induced transcription from a regulon that is distinct from the regulon induced by OxyR oxidation. Furthermore, products unique to the anaerobic regulon protected against S-nitrosothiols, and anaerobic growth of E. coli lacking OxyR was impaired on nitrate. Thus, OxyR serves as a master regulator of S-nitrosylation, and alternative posttranslational modifications of OxyR control distinct transcriptional responses.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3837355/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3837355/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Seth, Divya -- Hausladen, Alfred -- Wang, Ya-Juan -- Stamler, Jonathan S -- R01 HL059130/HL/NHLBI NIH HHS/ -- R01 HL095463/HL/NHLBI NIH HHS/ -- R01 HL096673/HL/NHLBI NIH HHS/ -- R01-HL059130/HL/NHLBI NIH HHS/ -- R01-HL095463/HL/NHLBI NIH HHS/ -- R01-HL096673/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 2012 Apr 27;336(6080):470-3. doi: 10.1126/science.1215643.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Transformative Molecular Medicine and Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals Case Medical Center, Cleveland, OH 44106, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22539721" target="_blank"〉PubMed〈/a〉
Keywords:
Anaerobiosis
;
Culture Media
;
Escherichia coli K12/genetics/growth & development/*metabolism
;
Escherichia coli Proteins/genetics/*metabolism
;
Nitrates/*metabolism
;
Nitric Oxide/metabolism
;
Nitrites/metabolism
;
Oxidation-Reduction
;
Promoter Regions, Genetic
;
Protein Processing, Post-Translational
;
Regulon
;
Repressor Proteins/genetics/*metabolism
;
S-Nitrosothiols/*metabolism
;
Transcriptional Activation
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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