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  • 1
    Electronic Resource
    Electronic Resource
    Uncoupled synthesis of histones and DNA during Friend cell differentiation (1978)
    [s.l.] : Nature Publishing Group
    Nature 276 (1978), S. 276-277 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Rate of DNA synthesis and appearance of newly synthesised proteins in the nuclei of n-butyrate-treated F4N cells. Ostertag's erythroleukaemic mouse spleen cell line F4N was used2 and cells were kept in culture conditions described previously10. For induction of the erythropoietic ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/276276a0
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  • 2
    Electronic Resource
    Electronic Resource
    Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers (2001)
    [s.l.] : Nature Publishing Group
    Nature structural biology 8 (2001), S. 606-610 
    Details
    ISSN: 1072-8368
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Single chromatin fibers were assembled directly in the flow cell of an optical tweezers setup. A single λ phage DNA molecule, suspended between two polystyrene beads, was exposed to a Xenopus laevis egg extract, leading to chromatin assembly with concomitant apparent shortening of the DNA ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/89646
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  • 3
    Electronic Resource
    Electronic Resource
    Matrix-associated DNA from maize is enriched in repetitive sequences (1992)
    Springer
    Plant cell reports 11 (1992), S. 355-358 
    Details
    ISSN: 1432-203X
    Keywords: Zea mays ; Matrix-associated ; DNA ; repetitive sequences ; DNA loops
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to elucidate some features of the topological organization of DNA within the plant nucleus, DNA fragments involved in the attachment of the DNA loops to the nuclear matrix in maize were studied. The matrix-associated DNA from dry embryo and meristematic cells after extensive digestion with DNase I and high salt treatment was about 2% of the total DNA, sized within the range of 50 and 250 bp. This DNA was found to be enriched in repetitive DNA sequences, both for nuclei from dry embryo and meristematic cells. The loop size of the DNA in cells of Zea mays appeared to be between 5 and 25 kbp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233365
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  • 4
    Electronic Resource
    Electronic Resource
    Antigenic structure of histone H1° (1991)
    Springer
    Molecular and cellular biochemistry 107 (1991), S. 161-168 
    Details
    ISSN: 1573-4919
    Keywords: antigenic determinants ; histone Hl° ; immunoblotting ; peptides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract In order to study the antigenic structure of histone Hl° the purified protein from mouse liver was subjected to different chemical and enzymatic treatments (CNBr, acetic acid, trypsin, chymotrypsin). The resulting peptides were fractionated in SDS-containing or acid-urea polyacrylamide gels, transferred by electroblotting onto nitrocellulose paper and probed with specific rabbit anti-H1° antiserum. The C-terminal fragments 99–193 (obtained following acetic acid hydrolysis) and 107–193 (obtained by chymotrypsin digestion) also exhibited strong immunoreactivity. Fragment 1–30 (CNBr cleavage) contained antigenic determinants while the shorter fragments 1–22 and 1–28 (acetic acid hydrolysis) failed to show any detectable reactivity. It was concluded that, in contrast to histone H5 whose reactivity is mainly concentrated to the globular domain of the molecule, the antigenic determinants in histone H1° are more or less evenly distributed along the polypeptide chain with the possible exception of the short unstructured N-nose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225519
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  • 5
    Electronic Resource
    Electronic Resource
    Accessibility of histone H1° and its structural domains to antibody binding in extended and folded chromatin (1990)
    Springer
    Molecular and cellular biochemistry 95 (1990), S. 167-175 
    Details
    ISSN: 1573-4919
    Keywords: antibody binding ; chromatin ; linker histones ; histone H1° ; structural domains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The aim of this work was to study the accessibility of histone H1° and its structural domains to antibody binding in high molecular mass chromatin fragments of different conformations. Three types of specific antibody populations were used: (1) anti-H1° which reacted with antigenic determinants situated along the whole polypeptide chain, (2) anti-GH5 or anti-GH1° which recognized epitopes located in the globular region of H1° and (3) anti-C-tail antibodies reacting specifically with fragment 99–193 of the protein molecule. The immunoreactivity of the chromatin-bound antigen was investigated by solid-phase ELISA performed on glutaraldehyde-cross-linked chromatin and by an inhibition assay carried out with native chromatin in solution. The results of both methods were unidirectional and showed that: (1) the accessibility of H1° did not change with the compaction of the fiber; (2) the G-domain was not accessible to antibodies either in the relaxed or in the condensed state of the fragments, (3) the binding of the C-terminus-specific antibodies was different for isolated monosomes and for the chromatin fiber and (4) the degree of exposure of the epitopes of H1° in chromatin was much less than that of histone H1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219975
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  • 6
    Electronic Resource
    Electronic Resource
    Antibodies specific to histone H1 inhibitin vitro transcription in isolated mammalian nuclei (1992)
    Springer
    Molecular and cellular biochemistry 110 (1992), S. 91-100 
    Details
    ISSN: 1573-4919
    Keywords: active chromatin ; anti-histone H1 antibodies ; histone H1 ; in vitro transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The issue of whether histone H1 is present in transcriptionally active chromatin has been approached by studying the effect specific anti-H1 antibodies have onin vitro transcription in isolated nuclei. To that end, the incorporation of radioactive RNA precursors into trichloroacetic acid-precipitable material was compared for control nuclei and nuclei that had been preincubated with specific anti-H1 antibody populations (whole sera, affinity-purified immunoglobulins and monovalent Fab fragments). The anti-H1 antibodies significantly and reproducibly inhibited the transcriptional activity in isolated nuclei. Experiments were also performed to exclude the possibility that the inhibition observed was due to some long-distance effect of the binding of the antibodies to chromatin. The results are interpreted as indicating that active gene chromatin does contain histone H1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02385010
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  • 7
    Electronic Resource
    Electronic Resource
    Immunochemical approaches to the study of histone H1 and high mobility group chromatin proteins (1990)
    Springer
    Molecular and cellular biochemistry 92 (1990), S. 1-22 
    Details
    ISSN: 1573-4919
    Keywords: histone H1 ; high mobility group proteins ; immunochemistry ; antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary This review is an attempt to summarize all existing data on histone H1 and high mobility group proteins obtained with immunochemical methods. The following issues are treated consecutively: production of specific antisera to these protein groups, antigenic structure of the polypeptide chains, use of antibodies for the identification, the quantitative estimation and the study of the tissue- and species-specificity of the proteins. Special attention is devoted to the studies of the localization of the respective antigens in the cell, the nucleus, the chromosomes and the interphase chromatin. The use of specific antibodies for the elucidation of the role these proteins play in such basic cellular processes as proliferation and differentiation, replication and transcription is also discussed. It becomes clear that the use of immunochemical approaches in the study of specific chromatin proteins both at the level of the protein molecule and at the level of chromatin can be a powerful tool for the resolution of a number of specific problems. The field is very promising and will undoubtedly develop intensely in the nearest future.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220715
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  • 8
    Electronic Resource
    Electronic Resource
    Lack of coupling between DNA and histone synthesis in growth-arrested Friend erythroleukemia cells (1981)
    Springer
    Molecular and cellular biochemistry 35 (1981), S. 49-53 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Histone synthesis was studied in Friend cells growth-arrested by culturing in isoleucine-deficient medium for 20–24 hours. Such cells are characterized by a very low level of DNA synthesis (5% of the controls). In contrast, the labelling of total nuclear proteins and of total histones continues at an unreduced level for many hours. At the same time there is no accumulation of histones in the cell nucleus, suggesting histone turnover. The behaviour of histone H1 differs from that of the nucleosomal histones, a fact speaking in favour of the existence of independent control mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358187
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  • 9
    Electronic Resource
    Electronic Resource
    DNA repair precedes replicative synthesis during early germination in maize (1987)
    Springer
    Plant molecular biology 10 (1987), S. 139-144 
    Details
    ISSN: 1573-5028
    Keywords: DNA synthesis ; germination ; single-strand breaks ; nucleoid sedimentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA synthesis was studied during germination by following the rate of incorporation of radioactive thymidine into high molecular weight DNA. A peak of DNA synthesis was observed between the 8th and the 12th hour, i.e. before the beginning of the semi-conservative replication of genomic DNA, accompanied by an increase in the DNA content of the embryo. By the use of nucleoid sedimentation and nick-translation it was shown that, during the first hours of germination, extensive repair occurs of the DNA single-strand breaks present in the dry embryo. As a result, the DNA of the 16-h-germinated embryo acquires the conformation typical of that of the root meristemic cells active in transcription and replication. In addition we have shown that cytoplasmic organelle (most probably mitochondrial) DNA synthesis is very active during the prereplicative state which confirms earlier microscopic data on mitochondrial biogenesis during early germination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016151
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  • 10
    Electronic Resource
    Electronic Resource
    Unusual DNA structures, chromatin and transcription (1994)
    New York, NY : Wiley-Blackwell
    BioEssays 16 (1994), S. 59-68 
    Details
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Extensive studies of DNA secondary structure during the past decade have shown that DNA is a dynamic molecule, whose structure depends on the underlying nucleotide sequence and is influenced by the environment and the overall DNA topology. Three major non-B-DNA structures have been described (Z-DNA, triplex DNA and cruciform DNA) which are stabilized by unconstrained negative supercoiling and can be formed under physiological conditions. In this essay we summarize the DNA primary structure features that are pertinent to the formation of these conformers and present data concerning the occurrence of these sequences in the eukaryotic genome. The evidence in favor of the existence of these unusual DNA structures in vivo is discussed. The effect of alternative non-B-DNA structures on the way DNA is organized in chromatin is considered, and this is followed by evaluation of the data relating these structures to eukaryotic transcription. Some possible mechanisms by which the effect of non-B structures on transcription might be exerted are proposed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bies.950160110
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