Publication Date:
2005-11-16
Description:
The notch family of transmembrane receptors play crucial roles in cell fate determination through interaction with the Delta/Serrate /Lag-2 family of ligands. The transcription of the hairyl enhancer of split (HES-1) gene is one of the known target of notch signaling. Notch1 is involved in the pathogenesis of T-acute lymphoblastic leukemia (T-ALL) carrying the very rare translocation t (7;9)(q34;q34.3). However, the function of Notch signaling in leukemogenesis is currently still unclear. To investigate the possible role of notch signaling in human acute leukemia,A quantitative real-time reverse transcriptase polymerase chain reaction (RQ-RT-PCR) method has been established for detecting expression levels of Notch1~2, Jagged1, Delta1, Hes1 and internal reference GAPDH in primary BM samples of 34 acute leukemia patients and 11 normal controls by LightCycler using TaqMan probe; 34 pretreated acute leukemia patients includes 16 acute lymphoblastic leukemia (7 T-ALL, 9 B-ALL) and 18 acute myeloblastic leukemia (10 M1,3 M2,3 M3 and 2 M5) In all of these cases the leukemia blast cells are more than 80%. The results demonstrated as the follows: The average expression levels of Notch1 in 16 ALL were statistically higher than that in 11 normal and 18 AML [ 13225.0±3306.3 (16) vs 1725.6±1237.8 (11) vs 2354.8±2985.6(18), F=7.85, P0.05); the average expression levels of Notch2 did not have statistically difference among ALL, normal and AML.[143671.7±372814.9 (16) vs 52178.2±36747.5 (11) vs 66520.2±62643.2 (18) F=0.15, P〉0.05 ]. The average expression levels of Jagged1 in 16 ALL were statistically less than that in 18 AML and 11 normal [129.9±353.8(16) vs 375.4±697.5(18) vs 2212.5±3371.7(11), F=20.23, P
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
Permalink