ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The specific rates of anaerobic solvent production by Clostridium acetobutylicum increased with increasing fermentor impeller speed from 190 to 340 rpm (NRe = 3.93 × 104). The maximum values were 5.54, 3.85, and 0.8 mmol/h · g cell for butanol, acetone, and ethanol, respectively. Corresponding rates for respective gases produced were 11.60 and 15.88 mmol/h · g cell for H2 and CO2. Further increases in agitation speed resulted in generally decreasing specific production rates to the point of inactive fermentation at 560 rpm. A competition observed between the cellular subsystems for butanol + butyric acid and biomass biosynthesis was evaluated through expressing the energetic yield coefficients. An imbalance between the production and outflux of the former metabolites is apparently further enhanced by a mechanical damage of the cells at high shear rates. A correlation was developed between the production of gases and solvents pointing at both H2-to-solvent as well as CO2-to-solvent ratios following the same pattern, peaking at 410 rpm.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260270905
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