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  • 1
    Electronic Resource
    Electronic Resource
    Simulation of Failure in Hydrating Cement Particles Systems (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 348-349 (Sept. 2007), p. 737-740 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The aim of this paper is to present the failure process of cement paste at different agessubjected to tensile loading. The input structure (cement paste) is generated by means of the cementhydration model HYMOSTRUC3D. A 2D-slice of this generated microstructure is subsequentlytransferred to the Delft Lattice Model, in which the failure process is analysed. Failure of cement pastemicrostructures at different degree of hydration, various water-cement ratios and different particlesize distributions are analysed and discussed in the paper
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/55/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.348-349.737.pdf
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  • 2
    Electronic Resource
    Electronic Resource
    Biolistic nuclear transformation of Saccharomyces cerevisiae and other fungi (1990)
    Springer
    Current genetics 17 (1990), S. 97-103 
    Details
    ISSN: 1432-0983
    Keywords: Fungal transformation ; Particle gun ; Microprojectiles ; Biolistic gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tungsten microprojectiles coated with nucleic acid and accelerated to velocities of approximately 500 m/s, can penetrate living cells and tissues with consequent expression of the introduced genes (Klein et al. 1987). Saccharomyces cerevisiae is used here as a model system to define the basic parameters governing the biolistic (biological-ballistic) delivery of DNA into cells. Among the physical factors affecting the efficiency of the process in yeast are the microprojectile's constitution, size, concentration and amount, and the procedure used for binding DNA to it. The biological parameters that affect the process include the cell's genotype, growth phase, plating density, and the osmotic composition of the medium during bombardment. By optimizing these physical and biological parameters, rates of transformation between 10-5 and 10-4 were achieved. Stable nuclear transformants result primarily from penetration of single particles of 0.5–0.65 μm in diameter, delivering on average 10–30 biologically active plasmids into the cell. The tungsten particles detectably increase the buoyant density of the transformants' progenitors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312852
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  • 3
    Electronic Resource
    Electronic Resource
    Silencing of TGF-β signalling by the pseudoreceptor BAMBI (1999)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 401 (1999), S. 480-485 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Members of the transforming growth factor-β (TGF-β) superfamily, including TGF-β, bone morphogenetic proteins (BMPs), activins and nodals, are vital for regulating growth and differentiation. These growth factors transduce their signals through pairs of transmembrane type I and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/46794
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  • 4
    Electronic Resource
    Electronic Resource
    Influence of Boundary Conditions on Pore Percolation in Model Cement Paste (2006)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 302-303 (Jan. 2006), p. 486-492 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Fresh model cement mixtures, with the same w/c ratio and particle size distribution, were simulated by the SPACE system that is based on a dynamic mixing algorithm. Thereupon, they were hydrated by the HYMOSTRUC 3D system. Boundary conditions were varied, rendering possible assessment of their influence on percolation of capillary porosity by serial sectioning and using the overlap of slices. Simulation results revealed increases in total porosity and in connected fraction ofcapillary pores due to the existence of aggregate. The de-percolation threshold of capillary porosity was found not only related to total porosity and image resolution, but also governed by the spatial distribution of capillary pores
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/50/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.302-303.486.pdf
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of cellulases on spontaneous fusion of maize protoplasts (1991)
    Springer
    Plant cell reports 10 (1991), S. 213-216 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of protoplast-isolating enzymes on spontaneous fusion of maize protoplasts (Zea mays L. cv. Black Mexican Sweet) was investigated using a convenient ethidium bromide nuclear staining procedure. After 2–2.5 hour digestion in an enzyme solution containing 1% Cellulysin, 0.5% Rhozyme, and 0.02% Pectolyase Y-23, 50–75% of the protoplasts contained multiple nuclei. The cellulase Cellulysin was identified as the factor causing the spontaneous protoplast fusion; when Cellulysin was replaced by CELF cellulase, most protoplasts were uninucleate. Calcium and other components in the enzyme solution did not affect spontaneous fusion. Cellulysin also increased the percentage of multinucleate protoplasts from rice and asparagus suspensions. Presence of multiple nuclei might affect genetic manipulations involving protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234298
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  • 6
    Electronic Resource
    Electronic Resource
    Tobacco (Nicotiana tobaccum) nuclear transgenics with high copy number can express NPTII driven by the chloroplast psbA promoter (1996)
    Springer
    Plant cell reports 15 (1996), S. 479-483 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract: A chloroplast expression vector containing the NPTII gene under the control of a psbA promoter (psbA-NPTII) was constructed, and was biolistically delivered into both suspension cells and leaf strips of tobacco (Nicotiana tabaccum). Analyses of subsequently recovered kanamycin-resistant transgenic plants indicate that the psbA-NPTII gene was not located in the chloroplast, but was in the nucleus in very high copy number. This conclusion was based upon results from: (1) Southern hybridization analyses of chloroplast and nuclear DNAs using NPTII, chloroplast-marker, and nuclear-marker probes; (2) pulse-field gel electrophoresis; and (3) kanamycin screening of sexual progenies. This study suggests that the nuclear expression of the NPTII gene may have been associated with many copies of the psbA-NPTII construction. Very high copy number in the nucleus might either allow NPTII expression from the otherwise inadequate psbA promoter, or might increase the chance of recombining with upstream tobacco regulatory sequences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050058
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  • 7
    Electronic Resource
    Electronic Resource
    Tobacco (Nicotiana tobaccum) nuclear transgenics with high copy number can express NPTII driven by the chloroplast psbA promoter (1996)
    Springer
    Plant cell reports 15 (1996), S. 479-483 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A chloroplast expression vector containing the NPTII gene under the control of apsbA promoter (psbA-NPTII) was constructed, and was biolistically delivered into both suspension cells and leaf strips of tobacco (Nicotiana tabaccum). Analyses of subsequently recovered kanamycin-resistant transgenic plants indicate that the psbA-NPTII gene was not located in the chloroplast, but was in the nucleus in very high copy number. This conclusion was based upon results from: (1) Southern hybridization analyses of chloroplast and nuclear DNAs using NPTII, chloroplast-marker, and nuclear-marker probes; (2) pulse-field gel electrophoresis; and (3) kanamycin screening of sexual progenies. This study suggests that the nuclear expression of the NPTII gene may have been associated with many copies of the psbA-NPTII construction. Very high copy number in the nucleus might either allow NPTII expression from the otherwise inadequate psbA promoter, or might increase the chance of recombining with upstream tobacco regulatory sequences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232978
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  • 8
    Electronic Resource
    Electronic Resource
    Optimization of delivery of foreign DNA into higher-plant chloroplasts (1990)
    Springer
    Plant molecular biology 15 (1990), S. 809-819 
    Details
    ISSN: 1573-5028
    Keywords: biolistic transformation ; β-glucuronidase ; microprojectiles ; tobacco chloroplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report here an efficient and highly reproducible delivery system, using an improved biolistic transformation device, that facilitates transient expression of β-glucuronidase (GUS) in chloroplasts of cultured tobacco suspension cells. Cultured tobacco cells collected on filter papers were bombarded with tungsten particles coated with pUC118 or pBI101.3 (negative controls), pBI505 (positive nuclear control) or a chloroplast expression vector (pHD203-GUS), and were assayed for GUS activity. No GUS activity was detected in cells bombarded with pUC118 or pBI101.3. Cells bombarded with pBI505 showed high levels of expression with blue color being distributed evenly throughout the whole cytosol of the transformants. pHD203-GUS was expressed exclusively in chloroplasts. We base this conclusion on: i) the procaryotic nature of the promoter used in the chloroplast expression vector; ii) delayed GUS staining; iii) localization of blue color within subcellular compartments corresponding to plastids in both shape and size; and iv) confirmation of organelle-specific expression of pHD203-GUS using PEG-mediated protoplast transformation. Chloroplast transformation efficiencies increased dramatically (about 200-fold) using an improved helium-driven biolistic device, as compared to the more commonly used gun powder charge-driven device. Using GUS as a reporter gene and the improved biolistic device, optimal bombardment conditions were established, consistently producing several hundred transient chloroplast transformants per Petri plate. Chloroplast transformation efficiency was found to be increased further (20-fold) with supplemental osmoticum (0.55 M sorbitol and 0.55 M mannitol) in the bombardment and incubation medium. This system provides a highly effective mechanism for introducing and expressing plasmid DNA within higher-plant chloroplasts, and the fact that GUS functions as an effective marker gene now makes many genetic studies possible which were not possible before.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039421
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  • 9
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    Selective removal of dishevelled by autophagy: A role of p62 (2011)
    Taylor & Francis
    Details
    Publication Date: 2011-03-01
    Print ISSN: 1554-8627
    Electronic ISSN: 1554-8635
    Topics: Biology
    Published by Taylor & Francis
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  • 10
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    QM/MM Studies on the Photophysical Mechanism of a Truncated Octocrylene Model (2019)
    American Chemical Society
    Details
    Publication Date: 2019-09-24
    Print ISSN: 1089-5639
    Electronic ISSN: 1520-5215
    Topics: Chemistry and Pharmacology , Physics
    Published by American Chemical Society
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