ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary The genepheA + coding chorismate niutase P-prephenate dehydratase, one of the regulatory enzymes of phenylalanine biosynthesis, was cloned into the down-stream of PR-PL tandem promoter. In this construction, both the native promoter-operator region and the attenuator region ofpheA + operon were eliminated so as to avoid the repression and attenuation ofpheA + gene expression. The expression ofpheA + gene was directed by PR-PL tandem promoter of bacteriophage lambda and controlled by a temperature sensitive repressor, cI857. It was shown that the expression as well as phenylalanine production was regulated by temperature. Maximum production of phenylalanine, 170 mg/l, was obtained at 40°C. The host strain, MC1065, produced a trace (4 mg/l) of phenylalanine at the same temperature.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00582417
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