Publication Date:
2012-04-08
Description:
Hydrogen peroxide (H 2 O 2 ), a substance involved in cellular oxidative stress, has been observed to induce an adaptive response, which is characterized by a protection against the toxic effect of H 2 O 2 at higher concentrations. However, the molecular mechanism for the adaptive response remains unclear. In particular, the existing reports on H 2 O 2 -induced adaptive response are limited to animal cells and human tumor cells, and relatively normal human cells have never been observed for an adaptive response to H 2 O 2 . In this study, a human embryo lung fibroblast (MRC-5) cell line was used to model an adaptive response to H 2 O 2 , and the relevant differential gene expressions by using fluoro mRNA differential display RT-PCR. The results showed significant suppression of cytotoxicity of H 2 O 2 (1100 μM, 1 h) after pretreatment of the cells with H 2 O 2 at lower concentrations (0.088–8.8 μM, 24 h), as indicated by cell survival, lactate dehydrogenase release, and the rate of apoptotic cells. Totally 60 mRNA components were differentially expressed compared to untreated cells, and five of them (sizing 400–600 bp) which demonstrated the greatest increase in expression were cloned and sequenced. They showed identity with known genes, such as BCL-2, eIF3S5, NDUFS4, and RPS10. Real time RT-PCR analysis of the five genes displayed a pattern of differential expression consistent with that by the last method. These five genes may be involved in the induction of adaptive response by H 2 O 2 in human cells, at least in this particular cell type. © 2012 Wiley Periodicals, Inc. Environ Toxicol, 2012.
Print ISSN:
1520-4081
Electronic ISSN:
1522-7278
Topics:
Energy, Environment Protection, Nuclear Power Engineering
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