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  • 1
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    Hazard assessment of sediments from a wetland system for treatment of landfill leachate using bioassays (2013)
    Elsevier
    Details
    Publication Date: 2013-11-01
    Print ISSN: 0147-6513
    Electronic ISSN: 1090-2414
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Elsevier
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Production and analysis of intraspecific somatic hybrids of potato (Solanum tuberosum L.)
    Amsterdam : Elsevier
    Plant Science 75 (1991), S. 107-115 
    Details
    ISSN: 0168-9452
    Keywords: DNA analysis by flow cytometry ; Solanum tuberosum ; isozyme analysis ; micromanipulation ; protoplast fusion ; somatic hybrids
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(91)90014-Y
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  • 3
    Electronic Resource
    Electronic Resource
    Somatic hybridization between anther-derived dihaploid clones of potato (Solanum tuberosum L.) and the identification of hybrid plants by isozyme analysis (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 49-56 
    Details
    ISSN: 1432-2242
    Keywords: Solanum tuberosum ; Protoplast fusion ; Somatic hybrids ; Isozymes ; Ploidy level
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Green mesophyll protoplasts of the dihaploid potato line 198∶2 (Solanum tuberosum L.) were fused with herbicide-bleached mesophyll protoplasts of the dihaploid potato line 67∶9 using a polyethylene glycol protocol. Heterokaryons were identified under a fluorescence microscope using the dual fluorescence of carboxyfluorescein-stained, herbicide-bleached protoplasts and the autofluorescence of green mesophyll protoplasts. About 20% of the protoplasts survived the fusion treatment, and the fusion frequency was 3%–4%. Unfused and fused protoplasts were mass cultured for 6 weeks after which vigorously growing calli were selected and transferred to shoot regeneration medium. Somatic hybrids were identified by a combination of five isozyme markers, and the ploidy level was determined by flow cytometry. Out of 15 calli that regenerated shoots, 6 plants derived from 2 different calli were identified as hexaploid somatic hybrids, while one morphologically deviant plant from a third callus was identified as a mixoploid that had lost some enzyme markers after 4 months of culturing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292315
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  • 4
    Electronic Resource
    Electronic Resource
    Regeneration and analysis of interspecific asymmetric potato –Solanum ssp hybrid plants selected by micromanipulation or fluorescence-activated cell sorting (FACS) (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 41-49 
    Details
    ISSN: 1432-2242
    Keywords: Key words Protoplasts ; Solanum tuberosum ; Solanum ssp. donor ; RAPD ; Chromosome elimination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Recipient protoplasts from three Solanum tuberosum genotypes, cv ‘Folva’ (2n=4x=48), cv ‘Matilda’ (4n) and ‘161 : 14’ (2n), were electrofused with X-ray-irradiated donor protoplasts from two wild species S. spegazzinii (2n) or S. microdontum×S. vernei (2n). Prior to fusion, protoplasts were fluorescence-labelled with either fluorescein diacetate or scopoletin. Fusion products were identified by dual fluorescence and selected by micromanipulation or fluorescence-activated cell sorting (FACS). All putative hybrid plants were analysed by the random amplified polymorphic DNA (RAPD) technique. Our analysis demonstrates that each asymmetric hybrid plant has an individual and stable profile of donor-specific RAPD bands. The irradiation of donor protoplasts hampered the growth of selected heterofusion products in a dose-dependent way. Irradiation resulted in donor chromosome elimination, but not in a dose dependent way, in the tested interval. In asymmetric hybrids with the S. spegazzinii donor 33–68% of the donor-specific RAPD bands were missing, indicating a similar level of chromosome elimination. In asymmetric hybrid plants with the S. microdontum×S. vernei donor 74–95% of the donor RAPD bands were missing. Chromosome countings revealed that these hybrids had chromosome numbers equal to or below the chromosome numbers found in the tetraploid recipients. This is the first time that highly asymmetric hybrid plants between two tetraploid potato recipients and the donor S. microdontum×S. vernei have been obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050530
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  • 5
    Electronic Resource
    Electronic Resource
    A cytogenetic and phenotypic characterization of somatic hybrid plants obtained after fusion of two different dihaploid clones of potato (Solatium tuberosum L.) (1992)
    Springer
    Theoretical and applied genetics 85 (1992), S. 470-479 
    Details
    ISSN: 1432-2242
    Keywords: Solanum tuberosum ; Somatic hybrids ; Cytology ; Morphology ; cpDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybrid plants of various ploidy levels obtained after chemical fusion between two dihaploid clones of potato Solanum tuberosum L. have been analysed by cytological, morphological and molecular methods. The hybrid nature of tetraploid and hexaploid plants and the genome dosage in hexaploid hybrids were confirmed by Giemsa C-banding. Tetraploid and hexaploid hybrids showed numerical as well as structural chromosome mutations. The latter occurred mainly in the nuclear organizing chromosome. The tetraploid hybrids were more vigorous than the dihaploid parents as demonstrated by an increase in height, enlargement of leaves, increase in the number of internodes, restored potential for flowering and increased tuber yield. The grouping of tetraploid somatic hybrids into various classes on the basis of leaf morphology revealed that plants with a full chromosome complement were more uniform than aneuploids. Many hexaploid somatic hybrids were also more vigorous than the dihaploid parents and could be grouped into two different classes on the basis of floral colour and tuber characteristics, the differences being due to their different dosage of parental genomes. Most of the tetraploid somatic hybrids showed pollen development halted at the tetrad stage as one of the parental clones contained a S. Stoloniferum cytoplasm. However, one tetraploid plant produced pollen grains with high viability. The chloroplast genome in the hybrid plants was determined by RFLP analysis. All of the hybrids had a cpDNA pattern identical to one parent, which contained either S. Tuberosum or S. Stoloniferum cpDNA. A slight preference for S. Tuberosum plastids were observed in hybrid plants. No correlation between pollen development and plastid type could be detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222329
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  • 6
    Electronic Resource
    Electronic Resource
    Characterisation of somatic hybrids between Solanum tuberosum and its frost-tolerant relative Solanum commersonil (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 1127-1132 
    Details
    ISSN: 1432-2242
    Keywords: Key words Somatic hybrids ; Fertility ; Frost tolerance ; S. tuberosum ; S. commersonii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Somatic hybrids between three dihaploid Solanum tuberosum (2n=2x=24) genotypes and the frost-tolerant, diploid, relative Solanum commersonii (2n=2x=24) were analysed for variation in morphological traits, fertility and frost tolerance. The somatic hybrids were more vigorous than their parents and in many ways resembled a tetraploid S. tuberosum. All of the hybrids flowered profusely, although the male fertility was largely dependent on the S. tuberosum genotype used. In one hybrid combination all plants were both male- and female-fertile, while in the other two combinations the majority of plants were male-sterile but female-fertile. The somatic hybrids showed an increase in direct frost tolerance when compared with the dihaploid S. tuberosum parents, and to a varying extent had gained the capacity to cold acclimate. These somatic hybrids will be used in breeding programmes involving repeated cycles of anther culture and somatic hybridisation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050672
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