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  • 1
    Electronic Resource
    Electronic Resource
    Further Studies on Phylogenetic Relationships in Secale: On the Origin of its Species (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 98 (1987), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Fourteen isozymatk loci have been analyzed in 23 samples of populations belonging to genus Secale by starch gel electrophoresis. The allelic frequencies for these loci have also been estimated and used in calculating genetic identity values between populations. Isozymatic data agree with the hypothesis that there are four species in the genus Secale: S. cereale, S. montanum, S. silvestre, and S. vavilovii. The characteristics of growth and outbreeding habits, and the isozymatic variability along with the previously known cytogenetic data, maintain S. montanum as the probable ancestor of the other three species: two are self-pollinated and monomorphic for isozymes (S. silvestre and S. vavilovii) and the other is open-pollinated and variable (S. cereale) and dearly distinguishable by its genetic variability from the other two. Finally, from isozymatic data it is not possible to distinguish between populations belonging to S. montanum and populations, belonging to. S. cereale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1987.tb01131.x
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  • 2
    Electronic Resource
    Electronic Resource
    Estimation of the Isozymatic Genetic Variability of a Spanish A vena sativa Germplasm Collection (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 106 (1991), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: An analysis of Spanish land races of Avena sativa from the Germplasm Bank of the I.N.I.A. was carried out using starch gel electrophoresis. Samples of 15 seedlings per accession were used for estimating the intra- and interpopulational genetic variability. Electrophoretic analyses were performed with crude extracts from 15 day-old seedling leaflets by means of eight isozymatic systems representing a total of ten genetic loci. Two systems were mono-morphic in all the accessions, while the other six showed polymorphism both within and between populations. Intrapopulational variability was particularly high in this collection, only 16 out of the 137 accessions being uniform for all the isozyme systems. Thus the material was shown to represent a high potential of genetic variability for breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1991.tb00514.x
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  • 3
    Electronic Resource
    Electronic Resource
    The structure of the rDNA intergenic spacer of Avena sativa L.: a comparative study (1994)
    Springer
    Plant molecular biology 25 (1994), S. 751-756 
    Details
    ISSN: 1573-5028
    Keywords: Avena sativa ; oats ; rDNA intergenic spacer ; sequence ; comparative study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sequence of the 18S–25S ribosomal RNA gene intergenic spacer (IGS) of Avena sativa was determined. DNA was cloned after polymerase chain reaction amplification of the IGS. The spacer of 3980 bp is composed of non-repeated sequences and five tandem arrays of repeated sequences, named A to E. Homology between oats IGS and other grass species was found. Tandem arrays D and E seem to be originated by duplication from single-copy sequences in related species. A transcription initiation site and putative sites of termination, processing and methylation were detected by computer-aided search. These sites resemble motifs conserved in the IGS of plant species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029613
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  • 4
    Electronic Resource
    Electronic Resource
    The inheritance of tetraploid wheat seed peroxidases (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 61-67 
    Details
    ISSN: 1432-2242
    Keywords: Triticum turgidum ; Tetraploid wheat ; Peroxidases ; Inheritance ; Linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Embryo and endosperm peroxidases from dry mature seeds of three subspecies of tetraploid wheat (Triticum turgidum L.) were subjected to genetic analysis. The inheritance of eight isozymes (embryo isozymes a2, d1, d2, e and f; and endosperm isozymes b, d and 4) were studied in F2's obtained from different wheat accessions. Simple monogenic inheritance producing three banded: one null segregation and two epistatic segregations (9∶7 and 15∶1) were found. In the case of isozymes b, d and 4, monogenic or epistatic segregation depended on the F2 analyzed. Segregation data indicated that at least 9 different loci would determine the peroxidase isozymes of tetraploid wheat seed, all the loci studied containing ‘null’ alleles. Furthermore, several loci determining embryo peroxidases were noticed to be mutually linked. All these data are discussed in context of the inheritance of seed peroxidases in hexaploid wheat and rye.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00278255
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  • 5
    Electronic Resource
    Electronic Resource
    Extent of genetic variability of endosperm esterases in Triticum aestivum L. 2n=6x=42 (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 728-734 
    Details
    ISSN: 1432-2242
    Keywords: Esterases ; Genetic variability ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic variability of endosperm esterase has been studied in 42 cultivars of Triticum aestivum L. 2n=6x=42. Different techniques, including sequential electrophoresis and electrofocusing, have been used with various substrates and esterase inhibitors. The electrophoretic patterns in each cultivar are described. Chromosomal location using the nullitetrasomic and ditelosomic lines of Chinese Spring was carried out in order to relate and/or locate the esterase genes to specific chromosomes. Most of the esterase isozymes located were in the long arm of the chromosomes of the homoeology group 3; but we have found six located in the short arms, five of them in the chromosome 3AS and one in the 3DS. This location increases the number of esterase genes described, because no esterase genes had been described so far in short arms of chromosomes of the homoeology group 3. The genetic control is discussed and, according to our results, between 12 and 15 loci, organized in five “compound loci”, control the endosperm esterases in wheat. Also one “modifier” gene modifying the mobility of two esterase bands and present in all the cultivars studied is postulated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262570
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic mapping of isozyme loci in Secale cereale L. (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 88-94 
    Details
    ISSN: 1432-2242
    Keywords: Secale cereale ; Rye ; Isozyme genes ; Genetic map ; Chromosomal assignment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genetics and linkage relationships of several isozymatic and morphological markers have been investigated in different cultivars of rye (Secale cereale L.). The inheritance and the variability among cultivars of three new isozymatic zones are described: GOT2 and LAP, each of them under the control of a two-allele single locus, namely Got2 and Lap, respectively; and 6PGD1 controlled by two loci, 6Pgd1a and 6Pgd1b, which have alleles in common. Four linkage groups have been found: Acp2-Acp3, Got3-Mdh2-Lper4, Mdh1-6Pgd2-Pgi2, and Pgm-Eper2-[Eper1-Eper3]. The assignment of these four groups to the chromosomes 7R, 3R, 1R, and 4R is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224020
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  • 7
    Electronic Resource
    Electronic Resource
    Ecogeographical distribution and differential adaptedness of multilocus allelic associations in Spanish Avena sativa L. (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 56-64 
    Details
    ISSN: 1432-2242
    Keywords: Avena sativa ; Genetic variability ; Multivariate analysis ; Multilocus associations ; Genetic resources
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We determined the nine-locus isozyme genotype of 267 landrace accessions of Avena sativa from 31 provinces of Spain. Our results establish that level of genetic variability is usually high both within and among accessions of this heavily self-fertilizing hexaploid grass and that multilocus genetic structure differs in various ecogeographical regions of Spain. We concluded that selection favoring different multilocus genotypes in different environments was the main integrating force that shaped the internal genetic structure of local populations as well as the overall adaptive landscape of A. sativa in Spain. Implications in genetic resource conservation and utilization are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222394
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  • 8
    Electronic Resource
    Electronic Resource
    Genetic variability of leaf esterases in Triticum aestivum L. 2n=6x=42 (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 673-678 
    Details
    ISSN: 1432-2242
    Keywords: Leaf esterases ; Genetic variability ; Triticumaestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Starch gel electrophoresis with two different buffer systems and several substrates and inhibitors have been used to study the electrophoretic variability of esterases in leaves of cultivars of Triticum aestivum. Each one of the buffer systems showed different levels of variability, according to the electrophoretic patterns. At the same time green and etiolated leaves showed different patterns in each buffer system. The variability was dependent upon the developmental stage of the leaves. According to the results from chromosomal location, the genes controlling esterases in green leaves were located in homoeology group 3, while the genes controlling esterases in etiolated leaves were in homoeology group 6. But both esterase isozymes showed a similar electrophoretic migration and a similar reponse to substrates and inhibitors. The possible origin of both sets of genes due to an interchromosomal duplication is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226882
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  • 9
    Electronic Resource
    Electronic Resource
    The chromosomal location of peroxidase isozymes of the wheat kernel (1979)
    Springer
    Theoretical and applied genetics 55 (1979), S. 73-76 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat, Peroxidases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The analysis of the individual parts of the Triticum aestivum L. kernel yields a total of 11 peroxidase isozymes: m, n, a, c, d1, d, d2, e, f, g and h (in order from faster to slower migration). Isozymes a, c and d are found in the endosperm (Ed) and seed coats (C), while m, n, d1, d2, e, f, g and h are peculiar to the embryo and scutellum (E + S). The use of the nullitetrasomic and ditellosomic series of ‘Chinese Spring’ wheat allows peroxidase isozymes to be associated with specific chromosome arms. Isozymes a, c and d (Ed) are associated with chromosome arms 7DS, 4BL and 7AS; whereas isozymes m, d2, e and f are associated with chromosome arms 3DS, 3BL, 3DL and 3DL, respecitvely. Thus, the E + S isozymes are associated with homoeology group 3 and the Ed isozymes with homoeology groups 7 (a and d isozymes) or 4 (c isozymes).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285193
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  • 10
    Electronic Resource
    Electronic Resource
    The chromosomal location of the embryo plus scutellum alcohol dehydrogenase isozymes in the hexaploid wheat kernel (1981)
    Springer
    Euphytica 30 (1981), S. 729-734 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Triticum turgidum ; durum ; wheat ; alcohol dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Analysis of individual parts of Triticum aestivum L. and T. turgidum var. durum kernels showed two classes of alcohol dehydrogenase patterns: 1) A three banded pattern (ADH-1, ADH-2 and ADH-3) for endosperm (Ed) and 2) a seven banded pattern (ADH-F1, ADH-F2, ADH-1, ADH-2, ADH-3, ADH-S1 and ADH-S2) for embryo (E), scutellum (S) and embryo plus scutellum (E+S). The use of nullitetrasomic and ditelosomic series of Chinese Spring wheat allows alcohol dehydrogenase to be associated with arms 4Aα, 4BL and 4DS. A five subunit forming dimer hypothesis is postulated to explain the newly reported seven isozyme pattern. Subunits α, β and δ are coded by chromosome arms 4Aα, 4BL and 4DS respectively, and the newly postulated ɛ and γ are coded also by chromosome arm 4BL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00038801
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