Publication Date:
2009-06-13
Description:
Innate immunity represents the first line of defense in animals. We report a genome-wide in vivo Drosophila RNA interference screen to uncover genes involved in susceptibility or resistance to intestinal infection with the bacterium Serratia marcescens. We first employed whole-organism gene suppression, followed by tissue-specific silencing in gut epithelium or hemocytes to identify several hundred genes involved in intestinal antibacterial immunity. Among the pathways identified, we showed that the JAK-STAT signaling pathway controls host defense in the gut by regulating stem cell proliferation and thus epithelial cell homeostasis. Therefore, we revealed multiple genes involved in antibacterial defense and the regulation of innate immunity.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2975362/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2975362/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cronin, Shane J F -- Nehme, Nadine T -- Limmer, Stefanie -- Liegeois, Samuel -- Pospisilik, J Andrew -- Schramek, Daniel -- Leibbrandt, Andreas -- Simoes, Ricardo de Matos -- Gruber, Susanne -- Puc, Urszula -- Ebersberger, Ingo -- Zoranovic, Tamara -- Neely, G Gregory -- von Haeseler, Arndt -- Ferrandon, Dominique -- Penninger, Josef M -- P01 AI044220/AI/NIAID NIH HHS/ -- P01 AI044220-10/AI/NIAID NIH HHS/ -- P01 AI44220/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2009 Jul 17;325(5938):340-3. doi: 10.1126/science.1173164. Epub 2009 Jun 11.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉IMBA, Institute of Molecular Biotechnology of the Austrian Academy of Sciences, A-1030 Vienna, Austria.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19520911" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Animals, Genetically Modified
;
Cell Proliferation
;
Drosophila Proteins/genetics/metabolism
;
Drosophila melanogaster/*genetics/immunology/*microbiology
;
Epithelial Cells/cytology/physiology
;
*Genome, Insect
;
Hemocytes/immunology/metabolism/microbiology
;
Homeostasis
;
Immunity, Innate/*genetics
;
Intestinal Mucosa/cytology/immunology/metabolism/microbiology
;
Janus Kinases/genetics/metabolism
;
Models, Animal
;
*RNA Interference
;
STAT Transcription Factors/genetics/metabolism
;
Serratia Infections/genetics/*immunology/microbiology
;
Serratia marcescens/*immunology/physiology
;
Signal Transduction
;
Stem Cells/cytology/physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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