Publication Date:
1993-02-19
Description:
The squid giant synapse was used to test the hypothesis that guanosine-5'-triphosphate (GTP)-binding proteins regulate the local distribution of synaptic vesicles within nerve terminals. Presynaptic injection of the nonhydrolyzable GTP analog GTP gamma S irreversibly inhibited neurotransmitter release without changing either the size of the calcium signals produced by presynaptic action potentials or the number of synaptic vesicles docked at presynaptic active zones. Neurotransmitter release was also inhibited by injection of the nonhydrolyzable guanosine diphosphate (GDP) analog GDP beta S but not by injection of AIF4-. These results suggest that a small molecular weight GTP-binding protein directs the docking of synaptic vesicles that occurs before calcium-dependent neurotransmitter release. Depletion of undocked synaptic vesicles by GTP gamma S indicates that additional GTP-binding proteins function in the terminal at other steps responsible for synaptic vesicle replenishment.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hess, S D -- Doroshenko, P A -- Augustine, G J -- NS-21624/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1993 Feb 19;259(5098):1169-72.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biological Sciences, University of Southern California.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8438167" target="_blank"〉PubMed〈/a〉
Keywords:
Aluminum/pharmacology
;
*Aluminum Compounds
;
Animals
;
Calcium/metabolism
;
Decapodiformes
;
*Fluorides
;
Fluorine/pharmacology
;
GTP-Binding Proteins/*physiology
;
Ganglia/physiology
;
Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
;
Guanosine Diphosphate/analogs & derivatives/metabolism/pharmacology
;
Guanosine Triphosphate/metabolism
;
In Vitro Techniques
;
Kinetics
;
Models, Neurological
;
Nerve Endings/physiology/ultrastructure
;
Signal Transduction/drug effects
;
Synaptic Vesicles/drug effects/*physiology/ultrastructure
;
Thionucleotides/pharmacology
;
Time Factors
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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