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  • 1
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cultured tobacco cells (Nicotiana tabacum L. cv. Wisconsin 38) adapted to NaCl exhibited significant morphological and physiological changes. Adapted cells remained smaller and more isodiametric at maturity than unadapted cells. The vacuole increased in size relative to the cytoplasm and an extensive network of transvacuolar membrane strands developed. These changes altered the surface contact area between the cytoplasm and the vacuole substantially. In addition, the network of Hechtian strands that anchor the cortical structure to the cell wall became more extensively branched possibly facilitating surface contact of the cytoplasm to the extracellular matrix. Many changes in membrane proteins could also be identified after NaCl adaptation. In particular, a 50-kDa protein that is associated with the plasma membrane and tonoplast was induced during adaptation. Immunocytochemical localization indicated that this 50-kDa protein is associated with Golgi vesicles. By immunoscreening using anti-50-kDa antibody, a 1.71-kb cDNA clone (p50C) was isolated from a λ-ZAP cDNA expression library. The sequence of p50C did not show any significant identity with other genes. Because of the very low abundance of the p50C message, quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to analyze p50C gene expression. Immunoblot and quantitative RT-PCR analyses indicated that the expression of this gene is regulated developmentally since the mRNA and protein increased with age in salt-adapted cells but decreased with age in unadapted cells. Also in tobacco plants, p50C mRNA was more abundant in younger leaves than in older leaves. The gene was responsive to NaCl in tobacco cells and to ABA in tobacco seedlings.
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  • 2
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effects of high temperature on accumulation of the 70-kDa heat shock protein (HSP70) and nucleoside diphosphate kinase (NDK) as well as two other proteins that have roles in the biosynthesis of storage proteins were examined during grain development. An HSP70 homolog and a 17-kDa NDK were co-purified from wheat endosperm, their identity verified, and a cDNA for an HSP70 expressed in endosperm was isolated. Wheat plants (Triticum aestivum, cvs Butte and Vulcan) were heat shocked at 40°C or exposed to maximum daily temperatures of 37 or 40°C during early or mid-grain fill. Antibodies and cDNA probes for BiP, HSP70, NDK and PDI were used to examine the effect of high temperatures on the accumulation of protein and mRNA in the endosperm. HSP70 mRNA levels increased substantially when plants were exposed to heat shock or to a 1-day gradual increase to 40°C. The effects of a 5-day heat treatment on mRNA levels were more complicated and depended on the developmental stage of the grain. A treatment that began at 7 days post-anthesis (DPA) decreased the level of mRNA for HSP70, BiP, PDI and NDK, whereas a treatment that began at 14 DPA slightly increased mRNA levels. The same treatments increased the accumulation of HSP70 but did not affect BiP, PDI, or NDK protein levels. This is the first detailed report on the effects of heat on mRNA and protein levels for HSP70 in a developing seed storage tissue.
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  • 3
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Temperature is one of the main external factors affecting anthocyanin accumulation in plant tissues: low temperatures cause an increase and elevated temperatures cause a decrease in anthocyanin concentration. Several metals have been shown to increase the half-life time of anthocyanins, by forming complexes with them. We studied the combined effect of elevated temperatures and increased metal concentrations on the accumulation of anthocyanins in aster ‘Sungal’ flowers. It has been found that magnesium treatment of aster plants or detached flower buds, partially prevents colour fading at elevated temperatures. Anthocyanin concentration of aster ‘Sungal’ flowers grown at 29°C/21°C day/night, respectively, was about half that of flowers grown at 17°C/9°C. The activity of phenylalanine ammonia-lyase (PAL) and chalcone isomerase (CHI) decreased as the temperature increased. Treatment of both whole plants and detached flower buds grown at elevated temperatures in the presence of magnesium salts, increased flower anthocyanin concentration by up to 80%. Measurement of magnesium following these treatments revealed an increased level of the metal in the petals, suggesting a direct effect. Magnesium treatment does not seem to cause increased synthesis of anthocyanin through a stress-related reaction, since the activities of both PAL and CHI did not increase due to this treatment. The results of this study show that increasing magnesium levels in aster petals prevents the deleterious effect of elevated temperatures on anthocyanin accumulation, thus enhancing flower colour.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    European journal of plant pathology 106 (2000), S. 633-638 
    ISSN: 1573-8469
    Keywords: Cucumis sativum ; integrated disease control ; Sphaerotheca fuliginea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A spray inoculation of the first leaf of 2-leaf stage cucumber plants with a non-pathogenic isolate of Alternaria cucumarina or Cladosporium fulvum before a challenge inoculation with the pathogen Sphaerotheca fuliginea induced systemic resistance to powdery mildew on leaves 2–5. Systemic resistance was expressed by a significant (p 〈 0.05) reduction in the number of powdery mildew colonies produced on each leaf of the induced plants, as compared with water-sprayed plants. Systemic resistance was evident when a prior inoculation with each of the inducing fungi was administered 1, 3 or 6 days before the challenge inoculation with S. fuliginea. Increasing the inoculum concentration of A. cucumarina or C. fulvum enhanced the systemic protection and provided up to 71.6% or 80.0% reduction, respectively, in the number of colonies produced on upper leaves, relative to controls. Increasing the inoculum concentration of S. fuliginea used for challenge inoculation, increased the number of powdery mildew colonies produced on both induced and non-induced plants. Pre-treated plants, however, were still better protected than controls, indicating that the level of systemic protection was related to the S. fuliginea inoculum concentration. The induction of systemic resistance against powdery mildew by biotic agents, facilitates the development of a wide range of disease management tools.
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  • 5
    ISSN: 1573-8469
    Keywords: induced resistance ; rast ; northern leaf blight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract One spray of 0.1 M aqueous solutions of NPK fertilizers on the upper sides of maize leaves 1, 2, and 3, 2–4 h prior to inoculation, induced systemic resistance (ISR) against northern leaf blight (NLB) caused byExserohilum turcicum andPuccinia sorghi which were developed on leaves 4, 5, 6, and 7. ISR was expressed as a reduction in the number and area of lesions ofE. turcicum and in the number of sporulating or non-sporulating pustules ofP. sorghi on leaves 4, 5, 6, and 7. The reduction in the number of NLB lesions ranged from 51% (KH2PO4) to 69% (K2HPO4) and their size reduction ranged from 73% (KNO3) to 91% (K2HPO4) as compared with water prayed plants. The reduction in the number of pustules ofP. sorghi ranged from 66 to 77%. Fertilizers consisting of various combinations of N, P and K in every case induce similar levels of protection in either host-pathogen system. The induced protection was evident regardless of the leaf position or the rate of NPK accumulation in the upper protected leaves. High fresh weight was detected in the induced plants which expressed the greatest induced protection against NLB and common rast. The possible dual use of NPK fertilizers — to supply nutrients to plants foliarly and at the same time to activate the mechanism(s) for induction of systemic protection toP. sorghi andE. turcicum in maize — is discussed.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    European journal of plant pathology 105 (1999), S. 351-361 
    ISSN: 1573-8469
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The non-protein amino acid BABA (DL-3-amino-n-butanoic acid, β-aminobutyric acid) is reported here to induce local and systemic resistance against downy mildew in grape leaves. Leaf discs of susceptible cultivars placed on BABA solutions and inoculated with Plasmopara viticola on the counter surface produced brownish restricted lesions below the inoculation site (Hypersensitive-like response, HR) which failed to support fungal sporulation. Histochemical analyses of such HR lesions revealed the accumulation of lignin-like deposits in the host cells. In contrast, water-treated inoculated discs produced expanded chlorotic lesions with profuse sporulation in which no lignin accumulation was observed. Mock-inoculated BABA-treated leaf discs showed no HR or lignin accumulation. Concentrations as low as 25 µg/ml (0.25 mM) of BABA sufficed to prevent tissue colonization with the fungus. Five other isomers of aminobutyric acid, namely L-2 aminobutyric acid, 2-amino isobutyric acid, DL-2-aminobutyric acid (AABA), DL-3-amino isobutyric acid, and 4-aminobutyric acid (GABA) gave no protection against the downy mildew fungus. Of the two (R and S) enantiomers of BABA only the R form was active in producing HR, suggesting a specific stereostructure requirement for activity. BABA could stop fungal colonization even when applied post-infectionally to leaf discs. Resistance of BABA-pulse-loaded leaf discs persisted for more than 14 days. BABA provided systemic protection against the disease when applied via the root system or via the lower leaves of grape plants. Application of 14C-BABA to a single leaf of intact plants showed the accumulation of the 14C label in upper leaves (and root tips), suggesting sink-oriented transport.
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  • 7
    ISSN: 1573-8469
    Keywords: induced resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A single spray of solutions of 0.005M H3BO3, 0.0025M CuSO4, and 0.0025 MnCl2, on the upper surface of the first true leaf of cucumber plants 2 h before inoculation with a conidial suspension of Sphaerotheca fuliginea, induced systemic protection against powdery mildew in leaves 2 and 3 without causing any damage on the induced leaf (first leaf). A similar level of systemic protection was observed when plants were induced by micronutrients, 2, 24 and 72 h before challenge with S. fuliginea. The level of protection induced by various concentrations varied from solution to solution. In general, the systemic protection induced by K2HPO4 was similar to that by the microelements. Spraying of a 1:1 mixture of phosphate and micronutrient solutions did not improve the systemic protection over that obtained with each of the solutions alone. Increasing the inoculum concentration of S. fuliginea increased the number of powdery mildew colonies produced on both induced and non-induced plants and has relatively affected the systemic protection on induced plants. A single foliar spray of micronutrient solutions, as a prophylactic treatment, on the upper surface of all the leaves of 3-leaf stage cucumber plants significantly inhibited powdery mildew development. A single spray of MnCl2 on leaf 1 elevated peroxidase activity in the soluble fraction and caused an enhancement of β-1,3-glucanase content in the ionically bound fractions of leaf 2 of non-inoculated plants. Forty-eight hours after inoculation, the level of both fractions of the enzymes increased in non-treated plants and decreased (β-1,3-glucanase) or remained unchanged (peroxidase) in treated (induced) plants as compared to non-treated plants. The possible mechanism for this protection, and the use of microelements and phosphate solutions as inducers for systemic protection and as agents for disease control are discussed.
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  • 8
    ISSN: 1573-8469
    Keywords: disease control ; foliar fertilizers ; Mangifera indica L. ; Oidium mangiferae ; synergistic interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Foliar sprays of solutions of di-potassium hydrogen orthophosphate, K2HPO4 (DKP) and potassium di-hydrogen orthophosphate, KH2PO4 (MKP), commercial systemic fungicides, and an alternating treatment with phosphate fertilizer and systemic fungicides inhibited development of the powdery mildew fungus, Oidium mangiferae, on flowers and bloom clusters of field-grown mango trees. The effectiveness of the alternation treatments with an appropriate systemic fungicide and 1% solution of mono-potassium phosphate (MKP) in controlling powdery mildew on bloom clusters was similar to that of the commercial treatment with the systemic fungicides. However, application of the systemic fungicides alone, on the same dates on which they were applied in the alternation treatment, without application of the phosphate treatment was significantly less effective in controlling the disease than either the phosphate or the alternation treatment. This indicates that the use of phosphate fertilizer has a significant role in disease control and that it can reduce the number of fungicide treatments necessary against powdery mildew by up to 50%. These results were confirmed in large-scale demonstration trials conducted in commercial orchards in 1994 and 1995. Our 1997 findings also revealed that tank-mix treatments of 1% MKP solution with half the recommended quantity of sterol inhibitor fungicide applied at 14-day intervals provided a protection against powdery mildew comparable with or superior to that given by the standard fungicides-based treatment applied at 7-day intervals. Tank-mix treatments of MKP (1%) with sterol inhibitor at the recommended rate or with the new strobilurin Kresoxym-methyl (BAS 490F, strobi), or the BAS 490F alone, were the most effective, and provided 〉95% protection against O. mangiferae, compared with the control. Phosphate solutions were found not to be phytotoxic to plant tissue. These treatments affected the yield of mango trees: a significantly lower yield was observed on control untreated trees, probably because of powdery mildew infection on flowers. The inhibitory effectiveness of phosphate salts makes them a potential major component of an integrated pest management program.
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  • 9
    Publication Date: 2009-06-10
    Print ISSN: 0021-8561
    Electronic ISSN: 1520-5118
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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  • 10
    Publication Date: 2020-04-24
    Description: Timorex Gold based on the essential tea tree oil (TTO) derived from the Australian tea tree oil (Melaleuca alternifolia) plant has demonstrated high efficacy and a strong curative activity against black Sigatoka in banana and controlled it in stages 1, 2, 3, and 4 of disease development. Transmission electron microscope (TEM) examination of infected leaf sections treated with Timorex Gold revealed disruption of the fungal cell membrane and destruction of the fungal cell wall in disease development stages 4 and 5. Mineral oil and the fungicide difenoconazole, when applied alone, had no curative effect and did not disrupt the fungal cell wall or membrane, similar to the untreated control tissue. A single spray of Timorex Gold effectively controlled and suppressed powdery mildew in cucumber by causing the disappearance of 99% of established colonies recorded 1 or 2 days after the application and was effective for up to 8 days after application. Scanning electron microscope (SEM) examination of infected and Timorex Gold-treated leaves indicated strong shrinkage and disruption of fungal hyphae and conidial cells. The curative and suppressive modes of action of the Timorex Gold may explain its success in controlling both diseases.
    Electronic ISSN: 2073-4395
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Economics
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