Publication Date:
2015-10-10
Description:
A father's lifetime experiences can be transmitted to his offspring to affect health and development. However, the mechanisms underlying paternal epigenetic transmission are unclear. Unlike in somatic cells, there are few nucleosomes in sperm, and their function in epigenetic inheritance is unknown. We generated transgenic mice in which overexpression of the histone H3 lysine 4 (H3K4) demethylase KDM1A (also known as LSD1) during spermatogenesis reduced H3K4 dimethylation in sperm. KDM1A overexpression in one generation severely impaired development and survivability of offspring. These defects persisted transgenerationally in the absence of KDM1A germline expression and were associated with altered RNA profiles in sperm and offspring. We show that epigenetic inheritance of aberrant development can be initiated by histone demethylase activity in developing sperm, without changes to DNA methylation at CpG-rich regions.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Siklenka, Keith -- Erkek, Serap -- Godmann, Maren -- Lambrot, Romain -- McGraw, Serge -- Lafleur, Christine -- Cohen, Tamara -- Xia, Jianguo -- Suderman, Matthew -- Hallett, Michael -- Trasler, Jacquetta -- Peters, Antoine H F M -- Kimmins, Sarah -- Canadian Institutes of Health Research/Canada -- New York, N.Y. -- Science. 2015 Nov 6;350(6261):aab2006. doi: 10.1126/science.aab2006. Epub 2015 Oct 8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology and Therapeutics, Faculty of Medicine, McGill University, Montreal, Quebec, Canada. ; Friedrich Miescher Institute for Biomedical Research (FMI), CH-4058 Basel, Switzerland. Faculty of Sciences, University of Basel, Basel, Switzerland. ; Department of Animal Science, Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Quebec, Canada. ; Department of Pediatrics, Faculty of Medicine, McGill University, Montreal, Quebec, Canada. ; Department of Animal Science, Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Quebec, Canada. Institute of Parasitology, Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Quebec, Canada. ; MRC Integrative Epidemiology Unity, School of Social and Community Medicine, University of Bristol, Bristol, UK. ; McGill Centre for Bioinformatics, School of Computer Science, Faculty of Science, McGill University, Montreal, Quebec, Canada. ; Department of Pediatrics, Faculty of Medicine, McGill University, Montreal, Quebec, Canada. Department of Human Genetics and Department of Pharmacology and Therapeutics, Research Institute of the McGill University Health Centre at the Montreal Children's Hospital, Montreal, Quebec, Canada. ; Friedrich Miescher Institute for Biomedical Research (FMI), CH-4058 Basel, Switzerland. Faculty of Sciences, University of Basel, Basel, Switzerland. sarah.kimmins@mcgill.ca antoine.peters@fmi.ch. ; Department of Pharmacology and Therapeutics, Faculty of Medicine, McGill University, Montreal, Quebec, Canada. Department of Animal Science, Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Quebec, Canada. sarah.kimmins@mcgill.ca antoine.peters@fmi.ch.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26449473" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Congenital Abnormalities/*genetics
;
CpG Islands
;
DNA Methylation
;
*Epigenesis, Genetic
;
Female
;
*Gene Expression Regulation, Developmental
;
Histone Demethylases/genetics/*metabolism
;
Histones/*metabolism
;
Male
;
Methylation
;
Mice
;
Mice, Transgenic
;
RNA, Messenger/metabolism
;
Spermatogenesis/*genetics
;
Spermatozoa/enzymology/*growth & development
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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