Publication Date:
2015-03-04
Description:
Evolution of large asexual cell populations underlies approximately 30% of deaths worldwide, including those caused by bacteria, fungi, parasites, and cancer. However, the dynamics underlying these evolutionary processes remain poorly understood because they involve many competing beneficial lineages, most of which never rise above extremely low frequencies in the population. To observe these normally hidden evolutionary dynamics, we constructed a sequencing-based ultra high-resolution lineage tracking system in Saccharomyces cerevisiae that allowed us to monitor the relative frequencies of approximately 500,000 lineages simultaneously. In contrast to some expectations, we found that the spectrum of fitness effects of beneficial mutations is neither exponential nor monotonic. Early adaptation is a predictable consequence of this spectrum and is strikingly reproducible, but the initial small-effect mutations are soon outcompeted by rarer large-effect mutations that result in variability between replicates. These results suggest that early evolutionary dynamics may be deterministic for a period of time before stochastic effects become important.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4426284/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4426284/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Levy, Sasha F -- Blundell, Jamie R -- Venkataram, Sandeep -- Petrov, Dmitri A -- Fisher, Daniel S -- Sherlock, Gavin -- 5-T32-HG-44-17/HG/NHGRI NIH HHS/ -- R01 HG003328/HG/NHGRI NIH HHS/ -- R25 GM067110/GM/NIGMS NIH HHS/ -- T32 HG000044/HG/NHGRI NIH HHS/ -- England -- Nature. 2015 Mar 12;519(7542):181-6. doi: 10.1038/nature14279. Epub 2015 Feb 25.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉1] Department of Genetics, Stanford University, Stanford, California 94305-5120, USA [2] Laufer Center for Physical and Quantitative Biology, Stony Brook University, Stony Brook, New York 11794-5252, USA [3] Department of Biochemistry and Cellular Biology, Stony Brook University, Stony Brook, New York 11794-5215, USA. ; 1] Department of Applied Physics, Stanford University, Stanford, California 94305, USA [2] Department of Biology, Stanford University, Stanford, California 94305, USA. ; Department of Biology, Stanford University, Stanford, California 94305, USA. ; Department of Genetics, Stanford University, Stanford, California 94305-5120, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25731169" target="_blank"〉PubMed〈/a〉
Keywords:
*Cell Lineage/genetics
;
Cell Tracking/*methods
;
DNA Barcoding, Taxonomic/methods
;
*Evolution, Molecular
;
Genetic Fitness/genetics
;
Mutagenesis/genetics
;
Mutation Rate
;
Saccharomyces cerevisiae/*cytology/genetics
;
Time Factors
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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