ISSN:
1435-604X
Keywords:
Radiation effects
;
In vitro maturation
;
In vitro fertilization
;
HeNe laser irradiation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Physics
,
Technology
Notes:
Abstract A study was made of the effects of low power laser irradiation on in vitro oocyte maturation rates and subsequent fertilization of immature bovine oocytes. Immature bovine oocytes from cows sacrificed at a slaughterhouse were irradiated with Helium-Neon laser (HeNe laser) irradiation at an energy density of 0.4 and 2.0 J cm−2. An oocyte group was left untreated, serving as control group. All oocytes were matured and fertilized in TCM-199 medium supplemented with 20% fetal calf serum (FCS). Maturation and fertilization rates obtained in the irradiated oocytes group were lower (p〈0.001 andp〈0.05, respectively) than those of the control group. Furthermore, the laser-treated oocytes showed important degenerative changes on both cytoplasm and chromosomes in comparison with untreated control oocytes which showed a homogenous cytoplasm and disperse chromosomes. It is concluded that the application of HeNe laser irradiation at 0.4 and 2.0 J cm−2 energy densities has a detrimental effect on in vitro maturation and fertilization process of immature bovine oocytes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02150848
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