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The effect of helium-neon laser irradiation on in vitro maturation and fertilization of immature bovine oocytes

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Abstract

A study was made of the effects of low power laser irradiation on in vitro oocyte maturation rates and subsequent fertilization of immature bovine oocytes. Immature bovine oocytes from cows sacrificed at a slaughterhouse were irradiated with Helium-Neon laser (HeNe laser) irradiation at an energy density of 0.4 and 2.0 J cm−2. An oocyte group was left untreated, serving as control group. All oocytes were matured and fertilized in TCM-199 medium supplemented with 20% fetal calf serum (FCS). Maturation and fertilization rates obtained in the irradiated oocytes group were lower (p<0.001 andp<0.05, respectively) than those of the control group. Furthermore, the laser-treated oocytes showed important degenerative changes on both cytoplasm and chromosomes in comparison with untreated control oocytes which showed a homogenous cytoplasm and disperse chromosomes. It is concluded that the application of HeNe laser irradiation at 0.4 and 2.0 J cm−2 energy densities has a detrimental effect on in vitro maturation and fertilization process of immature bovine oocytes.

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Authors and Affiliations

  1. Department of Genetics, Laboratory of Cytogenetics, Faculty of Veterinary Medicine, University of Cordoba, Spain

    J. M. Ocaña Quero & M. Moreno Millan

  2. Department of Animal Pathology, Faculty of Veterinary Medicine, University of Cordoba, Spain

    R. J. Gomez Villamandos & J. M. Santisteban Valenzuela

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  1. J. M. Ocaña Quero
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  2. R. J. Gomez Villamandos
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  3. M. Moreno Millan
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  4. J. M. Santisteban Valenzuela
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Quero, J.M.O., Villamandos, R.J.G., Millan, M.M. et al. The effect of helium-neon laser irradiation on in vitro maturation and fertilization of immature bovine oocytes. Laser Med Sci 10, 113–119 (1995). https://doi.org/10.1007/BF02150848

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  • DOI: https://doi.org/10.1007/BF02150848

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