Publication Date:
2003-06-07
Description:
Although the role of Toll-like receptors in extracellular bacterial sensing has been investigated intensively, intracellular detection of bacteria through Nod molecules remains largely uncharacterized. Here, we show that human Nod1 specifically detects a unique diaminopimelate-containing N-acetylglucosamine-N-acetylmuramic acid (GlcNAc-MurNAc) tripeptide motif found in Gram-negative bacterial peptidoglycan, resulting in activation of the transcription factor NF-kappaB pathway. Moreover, we show that in epithelial cells (which represent the first line of defense against invasive pathogens), Nod1is indispensable for intracellular Gram-negative bacterial sensing.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Girardin, Stephen E -- Boneca, Ivo G -- Carneiro, Leticia A M -- Antignac, Aude -- Jehanno, Muguette -- Viala, Jerome -- Tedin, Karsten -- Taha, Muhamed-Kheir -- Labigne, Agnes -- Zahringer, Ulrich -- Coyle, Anthony J -- DiStefano, Peter S -- Bertin, John -- Sansonetti, Philippe J -- Philpott, Dana J -- New York, N.Y. -- Science. 2003 Jun 6;300(5625):1584-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Unite de Pathogenie Microbienne Moleculaire, INSERM U389, Institut Pasteur, 28, Rue du Dr. Roux, 75724Paris Cedex 15, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12791997" target="_blank"〉PubMed〈/a〉
Keywords:
*Adaptor Proteins, Signal Transducing
;
Amino Acid Motifs
;
Animals
;
Antigens, Differentiation/metabolism
;
Carrier Proteins/chemistry/metabolism/*physiology
;
Cell Line
;
Cytoplasm/microbiology
;
Epithelial Cells/metabolism/microbiology
;
Gram-Negative Bacteria/*chemistry/immunology
;
Gram-Positive Bacteria/chemistry/immunology
;
Humans
;
Immunity, Innate
;
Interleukin-8/metabolism
;
*Intracellular Signaling Peptides and Proteins
;
Lipopolysaccharides/pharmacology
;
Mice
;
Myeloid Differentiation Factor 88
;
NF-kappa B/chemistry/metabolism
;
Nod1 Signaling Adaptor Protein
;
Nod2 Signaling Adaptor Protein
;
Oligopeptides/*analysis/chemistry
;
Peptidoglycan/*chemistry/pharmacology
;
Protein Structure, Tertiary
;
Receptors, Immunologic/metabolism
;
Signal Transduction
;
Trisaccharides/*analysis/chemistry
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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