ISSN:
1432-2048
Keywords:
Ethylene binding
;
Hormone binding
;
Phaseolus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The preparation is described of a cell-free system from developing cotyledons of Phaseolus vulgaris cv. Canadian Wonder which is capable of binding ethylene. The binding is saturable and the apparent dissociation constant for ethylene is 6.4·10-10 M in solution. The binding site is associated with subcellular particles and treatment with Triton X-100 results in substantial solubilisation of the activity. The kinetics of association and dissociation of the ligand and the binding site are described. The system is heat labile and binding activity is diminished by treatment with some proteolytic enzymes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00388129
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