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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 342 (1989), S. 834-836 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The occurrence of Beggiatoa in quantities several orders of magnitude higher than those previously observed in nature or achieved in the laboratory is not only highly unusual but could also provide physiological and technical information. In fact, there is no other example of a virtual bacterial ...
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 136 (1983), S. 262-269 
    ISSN: 1432-072X
    Keywords: Chemoautotroph ; Facultative ; Beggiatoa sp. ; Sulfide gradient cultures ; Microaerobic ; Marine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A recently isolated strain of Beggiatoa, MS-81-6 (cf. alba), was tested for chemoautotrophic growth in semi-solid (0.2% agar) sulfide/oxygen gradient cultures. The organism grew in a horizontal layer, the distance from the air/medium interface depending on sulfide concentrations and changing with time. Optimal growth as a gradient organism was based on a preference for reduced oxygen concentrations and a limited sulfide tolerance in combination with gliding motility. In gradient cultures chemoautotrophic growth was demonstrated by the following criteria: (1) biomass yield (protein) increased with increasing sulfide concentration, and estimated molar growth yields agreed with those for other sulfide-grown chemoautotrophs; (2) approximately 90% of total cell carbon and protein carbon were fixed from carbon dioxide; (3) the CO2-fixing enzyme, ribulosebisphosphate carboxylase, was present in cell-free extracts at a level typical of chemoautotrophs; (4) acidification of the medium, apparently linked to utilization of internal So granules, accompanied the later phase of growth. The ability to grow on acetate in the absence of a source of reduced sulfur renders the organism facultatively chemoautotrophic.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 133 (1982), S. 172-177 
    ISSN: 1432-072X
    Keywords: Beggiatoa ; Nitrogen fixation ; Acetylene reduction ; Nitrate assimilation ; Microaerobic ; Isolation of marine strains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four newly isolated marine strains of Beggiatoa and five freshwater strains were tested for nitrogen fixation in slush agar medium. All strains reduced acetylene when grown microaerobically in media containing a reduced sulfur source and lacking added combined nitrogen. The addition of 2 mmol N, as nitrate or ammonium salts, completely inhibited this reduction. Although not optimized for temperature or cell density, acetylene reduction rates ranged from 3.2 to 12 nmol·mg prot-1 min-1. Two freshwater strains did not grow well or reduce acetylene in medium lacking combined nitrogen if sulfide was replaced by thiosulfate. Two other strains grew well in liquid media lacking both combined nitrogen and reduced sulfur compounds but only under lowered concentrations of air. All freshwater strains grew well in medium containing nitrate as the combined nitrogen source. Since they did not reduce acetylene under these conditions, we infer that they can assimilate nitrate.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 131 (1982), S. 146-155 
    ISSN: 1432-072X
    Keywords: Beggiatoa ; Blue light response ; Colorless sulfur bacteria ; Step-up phobic light response ; Weber Law response ; Diel vertical migration ; Receptor pigment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Studies on nativeBeggiatoa demonstrated diel vertical migration into, and out of, sediments at the bottom of warm spring pools. Laboratory experiments withBeggiatoa in natural sediments suggested that high light was the cause of the downward movement. The nature of this presumed photomotion was clarified by microscopic observation of individual filaments of nativeBeggiatoa at light/dark boundaries where the light was varied in intensity and quality. Using “white light”, a negative photo-response was demonstrated, and a dose-response curve was constructed which indicates an increasing response to light over three orders of magnitude of intensity. A coarse action spectrum implicated a pigment with a peak in the blue region as the receptor. Pure culture studies showed the negative response to be a step-up phobic one. The light intensity increase necessary to invoke reversals was a smaller percentage of the initial intensity for higher initial intensities. The light intensity levels and gradient strengths necessary to evoke reversals in single filaments were consistent with the hypothesis that the step-up response accounts for the disappearance in the field. This response has adaptive significance since full sunlight was completely inhibitory toBeggiatoa growth, even when filaments were aggregated in tufts. Dilute suspensions were also inhibited by as little as 5000 lux (fluorescent lamps).
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  • 5
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The zonation and structure of phototrophic microbial mats were studied along two thermal gradients in sulfide-rich hot springs of southwest Iceland. The green, filamentous bacteriumChloroflexus and the unicellular, “high-temperature form” (HTF) ofMastigocladus formed mats growing up to a temperature limit of 62–66°C. The dominant phototrophs wereChloroflexus sp.,Mastigocladus laminosus, andPhormidium laminosum, respectively, at the three temperature intervals: 〉60°C, 60°C to 55–50°C, and 〈55–50°C. AChloroflexus mat growing at 60°C under 60μM H2S was anoxic in the light with the exception of a 0.5 mm thick band of HTFMastigocladus which produced oxygen. The oxygenic photosynthesis of these H2S-sensitive cyanobacteria was probably dependent on a preceding sulfide depletion by the anoxygenicChloroflexus. Measurements of spectral radiance gradients with a fiberoptic microprobe showed maximum light attenuation by carotenoids and bacteriochlorophyllC. AM. laminosus mat growing at 52°C was oxic throughout and showed maximum light attenuation by carotenoids, chlorophyllA, and phycocyanin, but no detectable phycoerythrocyanin absorption.
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  • 6
    ISSN: 1573-2959
    Keywords: mercury ; mining ; monitoring ; aquatic ; acid mine drainage ; floc ; Clear Lake ; remediation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Mercury (Hg) in the aquatic ecosystem of Clear Lake has been documented since the 1970s when fishes were found to have elevated levels of toxic methyl mercury (meHg). Mining practices at the Sulphur Bank Mercury Mine (active intermittently from 1872–1957) along the shoreline of Clear Lake included the bulldozing of waste rock and overburden ore into the shallow nearshore regions of the lake and the creation of steeply sloped piles of waste rock at the water's edge. This process, plus erosion of the waste rock piles, resulted in the accumulation of an estimated 100 metric tons of Hg in Clear Lake. A monitoring program to assess Hg in Clear Lake was established in 1992, and conducted continuously from 1994. Drought conditions in California had persisted for ca. 6 yrs prior to 1992, when the U.S. Environmental Protection Agency (USEPA) remediated the steeply sloped eroding waste rock piles, which appeared to reduce sediment Hg concentrations significantly. In April 1995, a white flocculent material was observed in Clear Lake adjacent to the mine and has been observed every year since, leading to the discovery of ongoing acid mine drainage (AMD), low pH fluids high in Hg and extremely high in sulfate. AMD is now believed to be the most likely cause of elevated meHg in Clear Lake. The discovery of this source of meHg production in Clear Lake, which will significantly influence remedial options, was only made possible by implementation of a diligent monitoring program.
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  • 7
    Publication Date: 2018-04-27
    Description: RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors RNA-guided transcriptional silencing in vivo with 〈i〉S. aureus〈/i〉 CRISPR-Cas9 repressors, Published online: 26 April 2018; doi:10.1038/s41467-018-04048-4 Repression of gene transcription using CRISPR-Cas9 has been achieved in vitro but not for delivery into adult animal models. Here, the authors use AAV8 to deliver the transcriptional repressor dSaCas9KRAB to the cholesterol regulator Pcsk9, and show repression up to 24 weeks and reduced cholesterol levels in mice.
    Electronic ISSN: 2041-1723
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General , Physics
    Published by Springer Nature
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  • 8
    Publication Date: 1982-01-01
    Print ISSN: 0302-8933
    Electronic ISSN: 1432-072X
    Topics: Biology
    Published by Springer
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  • 9
    Publication Date: 1983-12-01
    Print ISSN: 0302-8933
    Electronic ISSN: 1432-072X
    Topics: Biology
    Published by Springer
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  • 10
    Publication Date: 1982-03-01
    Print ISSN: 0302-8933
    Electronic ISSN: 1432-072X
    Topics: Biology
    Published by Springer
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