Publication Date:
2012-01-01
Description:
Background. Global concerns have been raised due to upward trend of Vancomycin IntermediateStaphylococcus aureus(VISA) and Vancomycin ResistantStaphylococcus aureus(VRSA) reports which mean casting doubt on the absolute effectiveness of the last line of antibiotic treatment forS. aureus, vancomycin. Hence, epidemiological evaluation can improve global health care policies.Methodology. 171 Isolates ofStaphylococcus aureuswere collected from different types of clinical samples in selected hospitals in Isfahan, Mashhad, and Tehran, Iran. Then, they were evaluated by agar screening, disk diffusion, and MIC method to determine their resistance to vancomycin and methicillin. The isolated VISA strains were then confirmed with genetic analysis by the evaluation ofmecAandvanAgenes,SCCmec, agr, andspatype, and also toxin profiles. MLST was also performed.Results and Conclusion. Our data indicated that 67% of isolatedS. aureusstrains were resistant to methicillin. Furthermore, five isolates (2.9%) had intermediate resistance to vancomycin (VISA). In contrast to usual association of VISA with MRSA strains, we found two isolates of MSSA-VISA. Therefore, our data suggests a probable parallel growing trend of VISA towards MSSA, along with MRSA strains. However, more samples are required to confirm these primarily data. Moreover, genetic analysis of the isolated VISA strains revealed that these strains are endemic Asian clones.
Print ISSN:
2090-7478
Electronic ISSN:
2090-7486
Topics:
Biology
,
Medicine
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