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  • 1
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The inheritance of resistance to fusarium wilt race 0 of chickpea and linked random amplified polymorphic DNA (RAPD) markers were studied in two F6:7 recombinant inbred line (RIL) populations. These RILs were developed from the crosses CA2156 × JG62 (susceptible × resistant) and CA2139 × JG62 (resistant × resistant), and were sown in a field infected with fusarium wilt race 0 in Beja (Tunisia) over 2 years. A1:1 resistant to susceptible ratio was found in the RIL population from the CA2156 × JG62 cross, indicating that a single gene with two alleles controlled resistance. In the second RIL population (CA2139 × JG62) a 3:1 resistant to susceptible ratio indicated that two genes were present and that either gene was sufficient to confer resistance. Linkage analysis showed a RAPD marker, OPJ20600, linked to resistance in both RIL populations, which is present in the resistant parent JG62.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Economic Theory 50 (1990), S. 155-174 
    ISSN: 0022-0531
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Economics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 85 (1993), S. 784-792 
    ISSN: 1432-2242
    Keywords: Wheat ; Rye ; Barley ; RFLP ; Biochemical markers ; Genetic maps
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic maps of the homoeologous group-2 chromosomes were constructed, comprising 114 loci in wheat and 34 loci in rye. These include the genes coding for sucrose synthase, sedoheptulose-1,7-bisphosphatase, a bZIP protein (EmBP-1), a peroxidase and an abscisic acid-induced protein (#7). Overall, gene orders are highly conserved in the genomes of wheat, barley and rye, except for the distal ends of chromosome arms 2BS and 2RS, which are involved in interchromosomal, probably evolutionary, translocations. Clustering of loci in the centromeric regions of the maps, resulting from the concentration of recombination events in the distal chromosomal regions, is observed in wheat and rye, but not in barley. Furthermore, loci for which homoeoloci can be detected in rye and barley tend to lie in the centromeric regions of the maps, while non-homoeologous and wheat-specific loci tend to be more evenly distributed over the genetic maps. Mapping of the group-2 chromosomes in the intervarietal ‘Timgalen’ x ‘RL4137’ cross revealed that the T. timopheevi chromosome segment introgressed into chromosome 2B in ‘Timgalen’ is preferentially transmitted. Recombination is also greatly reduced in that segment.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 92 (1996), S. 273-277 
    ISSN: 1432-2242
    Keywords: Key words Rosa sp.  ;  RAPDs markers  ;  Polymerase chain reaction  ;  Phylogenetic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Nineteen species of rose (Rosa sp.) were analysed using Random Amplified Polymorphic DNA markers (RAPD). Each 10-base-long arbitrary primer produced a specific DNA banding pattern that grouped plants belonging to the same species and botanical sections as predicted from their genetic background. One hundred and seventy-five amplification products were examined by cluster analysis to assess the genetic relationships among species and their genetic distances. All of the accessions belonging to 1 species grouped together before branching to other species. Dendrograms constructed for intra- and inter-specific studies showed a good correlation with previous classifications by different authors based on morphological and cariological studies. Our results show that the RAPD technique is a sensitive and precise tool for genomic analysis in rose, being useful in assigning unclassified accessions to specific taxonomic groups or else allowing accessions classified by traditional criteria to be re-classified.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 92 (1996), S. 273-277 
    ISSN: 1432-2242
    Keywords: Rosa sp. ; RAPDs markers ; Polymerase chain reaction ; Phylogenetic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nineteen species of rose (Rosa sp.) were analysed using Random Amplified Polymorphic DNA markers (RAPD). Each 10-base-long arbitrary primer produced a specific DNA banding pattern that grouped plants belonging to the same species and botanical sections as predicted from their genetic background. One hundred and seventy-five amplification products were examined by cluster analysis to assess the genetic relationships among species and their genetic distances. All of the accessions belonging to 1 species grouped together before branching to other species. Dendrograms constructed for intra- and inter-specific studies showed a good correlation with previous classifications by different authors based on morphological and cariological studies. Our results show that the RAPD technique is a sensitive and precise tool for genomic analysis in rose, being useful in assigning unclassified accessions to specific taxonomic groups or else allowing accessions classified by traditional criteria to be re-classified.
    Type of Medium: Electronic Resource
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  • 6
    Publication Date: 2005-06-22
    Print ISSN: 0925-9864
    Electronic ISSN: 1573-5109
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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  • 7
    Publication Date: 1986-11-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 8
    Publication Date: 2010-12-13
    Description: SUMMARYCharacterization of plant varieties is traditionally based on phenotypic observation. However, some varieties have very similar morphological characteristics, which make it difficult to distinguish between them. The present study employed 15 microsatellite markers distributed across all linkage groups (LG) of the chickpea genetic map to characterize 32 commercial chickpea cultivars and determine the usefulness of these markers for cultivar identification. These markers showed a high level of polymorphism; a total of 154 different alleles were detected, with a mean of 10·3 alleles per locus. The polymorphic information content (PIC) value ranged from 0·455 to 0·897. All the markers, with the exception of TA130, TA135 and TA144, were considered to be informative (PIC〉0·7), indicating their potential usefulness for cultivar identification. A subset of markers (TA186, TA200, TA106, TA113, TA117 and TA30) was sufficient to identify all the cultivars studied. In order to confirm their discriminatory power, 16 unreleased chickpea cultivars (V1–V16) were screened and all of them presented different patterns. Therefore, these microsatellites can be regarded as a reference set for chickpea cultivar identification and their profiles can be used as a DNA fingerprint for each registered cultivar, avoiding redundancy of identical cultivars as well as to protect breeders' rights.
    Print ISSN: 0021-8596
    Electronic ISSN: 1469-5146
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 9
    Publication Date: 2013-12-16
    Description: SUMMARYThe extent to which markers have been used in chickpea breeding programmes has not been clearly determined. In the current study, phenotypic and marker-assisted selection (MAS) were employed to select blight resistant genotypes, comparing the effectiveness of both methods. The phenotypic evaluation showed that the resistance could be recessive in the material employed. However, the high distorted segregation towards the susceptible parent detected on linkage group four (LG4) could also explain the phenotype distribution of resistance. Phenotypic selection in F2:4 and F2:5 generations lead to an increase in the frequency of the allele associated with the resistance of the markers CaETR and GAA47, indicating the usefulness of these markers for MAS. The markers TA72 and SCY17 could be also useful for MAS but the high distorted segregation towards the susceptible parent in the region where these markers are located could explain their low effectiveness. The costs associated with phenotypic selection and MAS for ascochyta blight resistance during three cycles of selection are presented in the current study, showing that MAS was more expensive than phenotypic selection. Nevertheless, the use of markers reduced the time taken to select resistant lines. The markers analysed in the current study were useful to select genotypes resistant to ascochyta blight in chickpea breeding programmes, allowing pyramiding genes or quantitative trait loci (QTL) related to different pathotypes. It is recommended that MAS should be employed in early generations of chickpea breeding programmes for the four QTL analysed because this makes it possible to develop populations with a high frequency of the favourable alleles conferring resistance to blight.
    Print ISSN: 0021-8596
    Electronic ISSN: 1469-5146
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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