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  • 1
    Publication Date: 2001-05-31
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lacombe, B -- Becker, D -- Hedrich, R -- DeSalle, R -- Hollmann, M -- Kwak, J M -- Schroeder, J I -- Le Novere, N -- Nam, H G -- Spalding, E P -- Tester, M -- Turano, F J -- Chiu, J -- Coruzzi, G -- New York, N.Y. -- Science. 2001 May 25;292(5521):1486-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11379626" target="_blank"〉PubMed〈/a〉
    Keywords: Arabidopsis/*genetics/metabolism ; *Genes, Plant ; Ion Channels/chemistry/classification/*genetics/metabolism ; Phylogeny ; Plants/genetics ; Receptors, Glutamate/chemistry/classification/*genetics/metabolism ; *Terminology as Topic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
    Publication Date: 2010-02-13
    Description: To feed the several billion people living on this planet, the production of high-quality food must increase with reduced inputs, but this accomplishment will be particularly challenging in the face of global environmental change. Plant breeders need to focus on traits with the greatest potential to increase yield. Hence, new technologies must be developed to accelerate breeding through improving genotyping and phenotyping methods and by increasing the available genetic diversity in breeding germplasm. The most gain will come from delivering these technologies in developing countries, but the technologies will have to be economically accessible and readily disseminated. Crop improvement through breeding brings immense value relative to investment and offers an effective approach to improving food security.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tester, Mark -- Langridge, Peter -- New York, N.Y. -- Science. 2010 Feb 12;327(5967):818-22. doi: 10.1126/science.1183700.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Australian Centre for Plant Functional Genomics, University of Adelaide, South Australia SA 5064, Australia. mark.tester@acpfg.com.au〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20150489" target="_blank"〉PubMed〈/a〉
    Keywords: Agriculture/*methods ; *Biotechnology ; *Breeding ; Crops, Agricultural/*genetics/growth & development ; Developing Countries ; Environment ; Food, Genetically Modified ; Genes, Plant ; Genetic Markers ; Genetic Techniques ; Genetic Variation ; Genotype ; Phenotype
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
    Publication Date: 2007-12-01
    Description: Both limiting and toxic soil concentrations of the essential micronutrient boron represent major limitations to crop production worldwide. We identified Bot1, a BOR1 ortholog, as the gene responsible for the superior boron-toxicity tolerance of the Algerian barley landrace Sahara 3771 (Sahara). Bot1 was located at the tolerance locus by high-resolution mapping. Compared to intolerant genotypes, Sahara contains about four times as many Bot1 gene copies, produces substantially more Bot1 transcript, and encodes a Bot1 protein with a higher capacity to provide tolerance in yeast. Bot1 transcript levels identified in barley tissues are consistent with a role in limiting the net entry of boron into the root and in the disposal of boron from leaves via hydathode guttation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sutton, Tim -- Baumann, Ute -- Hayes, Julie -- Collins, Nicholas C -- Shi, Bu-Jun -- Schnurbusch, Thorsten -- Hay, Alison -- Mayo, Gwenda -- Pallotta, Margaret -- Tester, Mark -- Langridge, Peter -- New York, N.Y. -- Science. 2007 Nov 30;318(5855):1446-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Private Mail Bag 1, Glen Osmond, South Australia 5064, Australia. tim.sutton@acpfg.com.au〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18048688" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Base Sequence ; Biological Transport ; Boron/metabolism/*toxicity ; Boron Compounds/*metabolism/*toxicity ; Chromosome Mapping ; *Genes, Plant ; Hordeum/*drug effects/*genetics/metabolism ; Membrane Transport Proteins/chemistry/*genetics/metabolism ; Molecular Sequence Data ; Plant Lectins/genetics/metabolism ; Plant Proteins/chemistry/genetics/metabolism ; Plant Roots/genetics/metabolism ; Quantitative Trait Loci ; Saccharomyces cerevisiae/genetics/growth & development/metabolism ; Transcription, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 16 (1993), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Measurements were made of the influx of 45Ca into internodal cells of Chara corallina in solutions containing high concentrations of NaCl. Increasing salinity in the range 4–100mol m−3 NaCl resulted in a doubling of Ca2+ influx at the plasmalemma. A time-course of Ca2+ influx in 50 mol m−3 NaCl, 0.5mol m−3 CaCl2 showed that while influx at the plasmalemma increased only 1.5-fold, influx to the vacuole increased by up to 15-fold. This was interpreted as being due to inhibition of active Ca2+ efflux from the cell. The stimulation of Ca2+ influx by increasing salinity appeared to be principally a response to reduced turgor since similar stimulations were obtained when turgor was reduced by NaCl, Na2SO4 or mannitol. When cells were plasmolysed Ca2+ influx increased by 10–20-fold. The increased permeability was relatively specific for Ca2+ and was inhibitable by La3+. Survival of cells in high salt conditions was increased by 30 mmol m−3 La3+, which inhibited Ca2+ influx. Paradoxically, survival can also be extended by increasing external Ca2+ which leads to a higher influx. Therefore, it seems unlikely that the ameliorative effect of Ca2+ on the sensitivity of plants to high NaCl is mediated by Ca2+ entry across the plasmalemma. It seems more likely that the principal role of Ca2+ under these conditions is exerted externally through the control of membrane voltage and permeability.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 10 (1987), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract There has been little written on the penetration of light through soil. In this review, we attempt to collate most of the work that has been published on this topic in order to stimulate further research and to clarify the often-confused literature.Light penetration can be measured directly with, for example, a spectroradiometer, or indirectly by using germination of light-sensitive seeds or the presence of growing algae as bioindicators. Although the penetration of light through soil is greatly affected by factors such as soil moisture content and particle size and colour, it generally appears that physiologically and ecologically significant amounts of light rarely penetrate more than 4–5 mm through the soil, and may often penetrate much less than this. Any penetration beyond 10 mm would generally not be significant, especially as most soils are covered with litter, algae or lichens, or are shaded from direct sunlight. However, for some light-stimulated geo-tropic responses of roots, which can be sensitive to very low fluences, the penetration of light to greater depths could well be significant. The role of light in soil in directing root growth is also discussed.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    FEBS Letters 333 (1993), S. 283-286 
    ISSN: 0014-5793
    Keywords: Aleurone ; Barley ; Capacitance measurement ; Endocytosis ; Exocytosis ; Hordeum vulgare ; Membrane turnover ; Pressure
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0014-5793
    Keywords: (Bacillus thuringiensis var. israelensis) ; Endotoxin, δ- ; Ion channel ; Planar lipid bilayer
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 91 (1994), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Ion channels are found in most plant membranes. They catalyse the rapid passive uniport of particular ions with varying selectivity. Planar lipid-bilayer (PLB) techniques have been developed to study the electrical activities of single ion channels in well-defined lipid and aqueous environments. They greatly facilitate both the biophysical and biochemical characterisation of ion channels and complement both conventional impaling electrode and membrane-patch voltage-clamping (patch-clamping) electrophysiological techniques applied in vivo. Bilayers can be formed across the end of patch-clamp pipettes or across apertures in specifically designed chambers. Ion channels in native membranes and purified, genetically altered or synthetic ion channels, proteins and peptides can all be studied in PLBs. The main applications of PLBs are (1) to study ion channels in membranes inaccessible to patch-clamp electrodes, (2) to provide a functional assay system during channel-protein purification and (3) to investigate the relationship between the molecular structure of ion channels and their conductance properties. In the present article we describe the techniques available for reconstitution and analysis of ion channels in PLBs and discuss how the PLB technique has been, and may be, useful to the study of plant ion channels.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-2048
    Keywords: Action potential ; Calcium channel effectors ; Channel kinetics ; Chara (K+ channel) ; Potassium channel ; Voltage clamp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The action of a wide range of drugs effective on Ca2+ channels in animal tissues has been measured on Ca2+ channels open during the action potential of the giant-celled green alga,Chara corallina. Of the organic effectors used, only the 1,4-dihydropyridines were found to inhibit reversibly Ca2+ influx, including, unexpectedly, Bay K 8644 and both isomers of 202–791. Methoxyverapamil (D-600), diltiazem, and the diphenylbutylpiperidines, fluspirilene and pimozide were found not to affect the Ca2+ influx. Conversely, bepridil greatly and irreversibly stimulated Ca2+ influx, and with time, stopped cytoplasmic streaming (which is sensitive to increases in cytoplasmic Ca2+). By apparently altering the cytoplasmic Ca2+ levels with various drugs, it was found that (with the exception of the inorganic cation, La3+) treatments likely to lead to an increase in cytoplasmic Ca2+ levels caused an increase in the rate of closure of the K+ channels. Similarly, treatments likely to lead to a decrease in cytoplasmic Ca2+ decreased the rate of K+ channel closure. The main effect of bepridil on the K+ channels was to increase the rate of voltage-dependent channel closure. The same effect was obtained upon increasing the external concentration of Ca2+, but it is likely that this was due to effects on the external face of the K+ channel. Addition of any of the 1,4-dihydropyridines had the opposite effect on the K+ channels, slowing the rate of channel closure. They sometimes also reduced K+ conductance, but this could well be a direct effect on the K+ channel; high concentrations (50 to 100 μM) of bepridil also reduced K+ conductance. No effect of photon irradiance or of abscisic acid could be consistently shown on the K+ channels. These results indicate a control of the gating of K+ channels by cytoplasmic Ca2+, with increased free Ca2+ levels leading to an increased rate of K+-channel closure. As well as inhibiting Ca2+ channels, it is suggested that La3+ acts on a Ca2+-binding site of the K+ channel, mimicking the effect of Ca2+ and increasing the rate of channel closure.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Planta 195 (1995), S. 478-488 
    ISSN: 1432-2048
    Keywords: Aluminium ; Calcium channel ; Planar lipid bilayer ; Plasma membrane ; Voltage clamp ; Triticum (Ca2+ channel)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new mechanism for calcium flux in wheat (Triticum aestivum L.) root cells has been characterized. Membrane vesicles were enriched in plasma membrane using aqueous-polymer two-phase partitioning and incorporated into artificial lipid bilayers, allowing characterization of single channels under voltage-clamp conditions. Membrane marker activities showed 74% and 83% purity in plasma membrane when expressed in terms of membrane area and activity, respectively. Since membrane vesicles obtained by aqueous-polymer two-phase partitioning yield a population of membrane vesicles of regular orientation, and vesicle fusion into planar lipid bilayers occurs in a defined manner, the orientation of the channel upon vesicle incorporation could be determined. Thus ionic activities and potentials could be controlled appropriately on what we propose to be the cytosolic (trans) and extracellular (cis) faces of the channel. The unitary conductance in symmetrical 1 mM CaCl2 was 27±0.4 (pS). The correlation between the theoretical and observed reversal potentials in asymmetrical conditions showed that the channel was highly selective for Ca2+ over Cl−. Experiments simulating physiological ionic conditions showed a PCa 2+/PK + of 17–26, decreasing in this range as the extracellular CaCl2 concentration increased from 0.1 to 1 mM. The channel was also permeable to the essential nutrient ions, Mg2+ and Mn2+. The open probability of the channel was strongly dependent on the membrane potential. Inactivation with time was observed at more negative membrane potentials, and was immediately reversed as soon as the membrane potential was decreased. At membrane potentials more negative than -130mV, the channel remained mainly in the closed state, suggesting that in vivo the channel would remain largely closed and would open only upon membrane depolarization. The channel was blocked by micromolar concentrations of extracellular verapamil and trivalent cations, Al3+ being the most effective of those tested. Exposure of the cytosolic and extracellular sides of the channel to inositol 1,4,5-trisphosphate had no effect on the channel activity. We suggest a plasma-membrane origin for the channel as shown by biochemical and electrophysiological evidence, and discuss possible physiological roles of this channel, both in Ca2+ uptake into roots and in signal transduction.
    Type of Medium: Electronic Resource
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