Publication Date:
2008-11-29
Description:
The Vibrio parahaemolyticus type III effector VopS is implicated in cell rounding and the collapse of the actin cytoskeleton by inhibiting Rho guanosine triphosphatases (GTPases). We found that VopS could act to covalently modify a conserved threonine residue on Rho, Rac, and Cdc42 with adenosine 5'-monophosphate (AMP). The resulting AMPylation prevented the interaction of Rho GTPases with downstream effectors, thereby inhibiting actin assembly in the infected cell. Eukaryotic proteins were also directly modified with AMP, potentially expanding the repertoire of posttranslational modifications for molecular signaling.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yarbrough, Melanie L -- Li, Yan -- Kinch, Lisa N -- Grishin, Nick V -- Ball, Haydn L -- Orth, Kim -- R01-AI056404/AI/NIAID NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2009 Jan 9;323(5911):269-72. doi: 10.1126/science.1166382. Epub 2008 Nov 27.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, University of Texas (UT) Southwestern Medical Center, Dallas, TX 75390, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19039103" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Monophosphate/*metabolism
;
Amino Acid Motifs
;
Amino Acid Sequence
;
Bacterial Proteins/chemistry/genetics/*metabolism
;
Binding Sites
;
Cell Shape
;
HeLa Cells
;
Humans
;
Molecular Sequence Data
;
Mutant Proteins/chemistry/metabolism
;
Phosphorylation
;
Protein Processing, Post-Translational
;
Protein Structure, Tertiary
;
Recombinant Fusion Proteins/metabolism
;
Signal Transduction
;
Threonine/chemistry/metabolism
;
Vibrio parahaemolyticus/*metabolism/pathogenicity
;
cdc42 GTP-Binding Protein/antagonists & inhibitors/chemistry/*metabolism
;
rac GTP-Binding Proteins/antagonists & inhibitors/chemistry/*metabolism
;
rho GTP-Binding Proteins/antagonists & inhibitors/chemistry/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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