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  • 1
    Electronic Resource
    Electronic Resource
    Relationships in pineapple by random amplified polymorphic DNA (RAPD) analysis (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Random amplified polymorphic DNA (RAPD) analysis was applied to eight commercial cultivars of pineapple, two intergroup hybrids and two wild species. Morphologically, pineapple is divided into the Cayenne, Queen, Spanish, Maipure and Abacaxi groups. Members of the first three groups have been analysed in this study. The cultivars ‘Tradsithong’, ‘Phuket’, ‘Sawee’ and ‘Tainan’, with spiny leaves, form the Queen group. In ‘Pattavia’, ‘Nanglae’ and ‘Petburi no. 2’ (Cayenne group), spines are confined to the leaf tips. ‘Intrachitdang’ is normally placed in the Spanish group, which is morphologically similar to the Queen group, but with inferior quality fruit. DNA amplification products were compared from 16 arbitrary 10-mer primers from which a dendrogram was constructed. The results confirmed morphological classifications for seven of the eight commercial cultivars, with the Queen and Cayenne groups as separate clusters. However, the cv. ‘Intrachitdang’ was more closely related to the Cayenne group. Two hybrids from reciprocal Cayenne × Queen group crosses, were more closely allied to the Queen group. The two wild species were outside the groups. RAPD analysis can be exploited to investigate relationships within pineapple germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00606.x
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of Pluronic F-68 on callus growth and protoplast plating efficiency of Solanum dulcamara (1991)
    Springer
    Plant cell reports 10 (1991), S. 52-54 
    Details
    ISSN: 1432-203X
    Keywords: Pluronic F-68 ; Callus growth ; Protoplasts ; Solanum dulcamara
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of the non-ionic surfactant, Pluronic F-68, on the growth of callus and protoplasts from Solanum dulcamara L. have been studied. Growth of callus was stimulated by addition of 0.1% (w/v) commercial grade Pluronic to culture medium, whereas lower concentrations (0.01% w/v) had no corresponding effect. In contrast, higher concentrations (1.0% w/v) of Pluronic inhibited callus growth. The mean plating efficiency of protoplasts grown at different densities (15 days after plating) was increased up to 26% following culture with 0.1% (w/v) Pluronic, while 0.01% (w/v) Pluronic was ineffective. Mean protoplast plating efficiency decreased by up to 32% following culture with 1.0% (w/v) Pluronic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233033
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  • 3
    Electronic Resource
    Electronic Resource
    Stimulation of shoot regeneration from jute cotyledons cultured with non-ionic surfactants and relationship to physico-chemical properties (1993)
    Springer
    Plant cell reports 13 (1993), S. 49-53 
    Details
    ISSN: 1432-203X
    Keywords: Corchorus capsularis ; jute ; cotyledons ; surfactants ; Pluronic F-68 ; Triton X-100 ; Tween 20 ; shoot regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects have been studied of the non-ionic surfactants, Plutonic F-68, Tween 20 or Triton X-100, on shoot regeneration from cultured jute (Corchorus capsularis L.) cotyledons with attached petioles. This group of non-ionic surfactants was selected in order to determine a possible relationship between the physico-chemical properties of individual compounds and their observable effects on plant morphogenesis in cultured jute cotyledons. Supplementation of culture medium with 0.001–0.5% (w/v) Pluronic F-68 increased the mean percentage of cotyledons producing shoots and the mean number of shoots/cotyledon, with maximal responses at 0.5% (w/v). By contrast, Tween 20 produced maximal effects at 0.001% (v/v), with inhibition of shoot formation at 0.5% (v/v). In both cases, phenotypically normal plants were recovered which could be grown to maturity. Culture of cotyledons with 0.001% (v/v) Triton X-100 similarly increased both the percentage of cotyledons producing shoots and the number of shoots/cotyledon. However, these shoots did not develop into mature plants. Additionally, shoots did not regenerate from cotyledons cultured with Triton at 0.01–0.5% (v/v). These results demonstrate mat there is an apparent relationship between the hydrophilic-hydrophobic (HLB) balance of surfactants which determine their cell permeabilising properties and consequently, their effects on morphogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232315
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  • 4
    Electronic Resource
    Electronic Resource
    Synergistic enhancement of protoplast growth by oxygenated perfluorocarbon and Pluronic F-68 (1994)
    Springer
    Plant cell reports 13 (1994), S. 251-255 
    Details
    ISSN: 1432-203X
    Keywords: Petunia hybrida ; protoplasts ; oxygen delivery ; perfluorochemicals ; Pluronic F-68 ; surfactant ; cell division
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cell suspension-derived protoplasts of albino Petunia hybrida were grown for 10 d at the interface between aqueous culture medium (KM8P) and an oxygenated (10 mbar for 15 min) perfluorocarbon liquid, perfluorodecalin. Protoplasts synthesised new cell walls and divided normally at the perfluorodecalin/culture medium interface, with a mean viability after 10 d of 〉 92.0%. The mean plating efficiency of protoplasts was elevated by 37% (P〈0.05) following culture at the perfluorodecalin/medium interface, but was unaltered by perfluorodecalin or oxygen separately. The mean plating efficiency of protoplasts cultured at the interface was further increased to a maximium of 52% above control, in the presence of oxygenated perfluorodecalin and KM8P medium supplemented with the non-ionic, co-polymer surfactant, Pluronic F-68 at 0.01% (w/v). These findings demonstrate the effectiveness of oxygenated perfluorodecalin for promoting protoplast growth, by facilitating oxygen delivery. The finding that Pluronic F-68 further increased the plating efficiency of protoplasts cultured at the perfluorocarbon/aqueous interface suggests that these agents improve growth through separate, but cumulative, mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233314
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  • 5
    Electronic Resource
    Electronic Resource
    Carbon dioxide-gassed fluorocarbon enhances micropropagation of rose (Rosa chinesis Jacq.) (1997)
    Springer
    Plant cell reports 17 (1997), S. 17-21 
    Details
    ISSN: 1432-203X
    Keywords: Key words Carbona dioxide ; Fluorocarbon ; Micropropagation ; Rosa chinesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The inert perfluorochemical (PFC) liquid, perfluorodecalin (Flutec PP6), has been used to increase the CO2 supply to cultured shoots of Rosa chinensis Jacq. cv. Baby Love. Culture of shoots in semi-solid medium overlaying CO2-gassed PFC (2 mbar; 5 min repeated every 7 days) for up to 42 days, increased biomass as reflected by significant (P〈0.01) increases in shoot number, number of leaves per shoot and mean shoot fresh weight. Additionally, there were significant (P〈0.01) increases in the number of roots and their fresh and dry weights following a further 10 days of culture on rooting medium prior to transfer of plants to the glasshouse. Treatment of cultured rose shoots with CO2-gassed PFC also significantly reduced (P〈0.01) the accumulation of phenolic compounds in roots. The total chlorophyll of aerial parts was unaffected, although total protein in shoots and roots was significantly (P〈0.01) lower than in the control. The biotechnological implications of this novel cultural régime are discussed for the micropropagation of woody species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050344
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  • 6
    Electronic Resource
    Electronic Resource
    Strategies for promoting division of cultured plant protoplasts: synergistic beneficial effects of haemoglobin (Erythrogen) and Pluronic F-68 (1997)
    Springer
    Plant cell reports 17 (1997), S. 13-16 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsPetunia hybrida ; Oxygen delivery ; Erythrogen ; Haemoglobin ; Pluronic F-68
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Novel approaches, involving supplementation of aqueous culture medium with haemoglobin solution (Erythrogen), in the presence or absence of the copolymer surfactant, Pluronic F-68, have been evaluated to facilitate cellular oxygen availability to promote mitotic division. Cell-suspension-derived protoplasts of albino Petunia hybrida cv. Comanche were cultured for up to 45 days in KM8P medium containing 1:50–1:500 (vol:vol) Erythrogen. The mean initial protoplast plating efficiency after 9 days with 1:50 Erythrogen (18.5%) was significantly greater (P〈0.05) than in untreated controls (11.3%). Supplementation of culture medium with 1:50 Erythrogen, together with 0.01% (wt/vol) Pluronic F-68, increased the mean plating efficiency after 9 days (24.4%) by 92% (P〈0.05) over the control (12.7%). These treatments also produced increases in biomass of protoplast-derived cells up to 2.5-fold greater than control (P〈0.01) over 80 days. Gassing the medium, containing 1:50 Erythrogen, with carbon monoxide abolished the increase in plating efficiency. There was no additional benefit of gassing Erythrogen-supplemented medium with 100% oxygen. The synergistic, beneficial effect of Erythrogen and Pluronic F-68 on protoplast division has implications for plant biotechnology utilising protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050343
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  • 7
    Electronic Resource
    Electronic Resource
    A stable transformation system for the ornamental plant, Datura meteloides D. C. (1999)
    Springer
    Plant cell reports 18 (1999), S. 554-560 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAgrobacterium-mediated transformation ; Datura meteloides ; Preculture period ; Transgene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A transformation system is described for Datura meteloides using the supervirulent Agrobacterium tumefaciens strain 1065, carrying both the β-glucuronidase (gusA) and neomycin phosphotransferase II (nptII) genes between the T-DNA border sequences of the binary vector. The importance of conditions such as the preculture period of the plant tissues, wounding, bacterial dilution and incubation time were evaluated in terms of transgenic plant production. A preculture period of 2–3 days, using a 1:20 or 1:10 (vol:vol) dilution of an overnight bacterial culture, resulted in optimum shoot regeneration, with 48% from a total of 576 explants regenerating transformed shoots. Expression of the gusA and nptII genes was confirmed by a GUS fluorometric assay and by NPTII ELISA. Southern analysis revealed the integration of both transgenes, which segregated as dominant Mendelian traits in seed progeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050621
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  • 8
    Electronic Resource
    Electronic Resource
    Acute physiological responses during blood substitution with colloidal gelatin in conscious rats (1984)
    Springer
    Cellular and molecular life sciences 40 (1984), S. 700-701 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of exchange-transfusion with a proprietary gelatin-based plasma volume expander, Haemaccel, have been investigated in conscious, chronically-catheterized rats. No adverse changes in basic cardiovascular or respiratory functions occurred during the procedure although an increase in intravascular fluid sodium but not potassium concentration was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01949730
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  • 9
    Electronic Resource
    Electronic Resource
    Changes in plasma enzyme concentrations in response to blood substitution with perfluorocarbon emulsion in the conscious rat (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 1228-1231 
    Details
    ISSN: 1420-9071
    Keywords: Blood replacement ; perfluorocarbon emulsion ; Fluosol-DA ; lactate dehydrogenase ; alkaline phosphatase ; rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of near total blood replacement with the proprietary perfluorocarbon (PFC)-based emulsion, Fluosol-DA 20%, on plasma concentrations of 2 enzymes, lactate dehydrogeanse (LDH) and alkaline phosphatase (ALP), have been examined in conscious, chronically catheterized rats. A pronounced fall in both plasma LDH (p〈0.05) an ALP (p〈0.01) occurred in response to exchange-transfusion. However, at 6 h following blood replacement, plasma concentrations of both enzymes had risen to values significantly greater than those measured immediately before perfusion. The observed changes in plasma LDH and ALP after blood replacement with Fluosol-DA indicated alterations in normal functioning of tissues from which these enzymes originate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01946398
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  • 10
    Electronic Resource
    Electronic Resource
    Effects of Pluronic F-68 on shoot regeneration from cultured jute cotyledons and on growth of transformed roots (1993)
    Springer
    Plant cell, tissue and organ culture 34 (1993), S. 133-140 
    Details
    ISSN: 1573-5044
    Keywords: Corchorus capsularis ; medium supplement ; necrosis ; organogenesis ; suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects have been studied of the non-ionic surfactant, Pluronic F-68, on the growth in culture of jute (Corchorus capsularis L.) cotyledons with attached petioles, cotyledon explants and transformed roots. Supplementation of culture medium with 0.001–0.5% (w/v) of either commercial grade Pluronic F-68 or a purified fraction prepared by passage through silica gel, stimulated shoot production from the petioles of C. capsularis var. D154 and C134 cotyledons. This effect was most marked in C134, because of the failure of control cotyledons to produce shoots in the absence of Pluronic. Plants regenerated from Pluronic-treated cotyledons were morphologically normal. Growth of transformed roots of C. capsularis var. D154 was stimulated in medium supplemented with commercial grade or purified Pluronic F-68, with maximum increases in both fresh and dry weights with 0.1% (w/v) of the surfactant. Roots cultured in the presence of Pluronic F-68 could be maintained without sub-culture for up to 70 days, whereas roots cultured in the absence of Pluronic required subculture every 7 days, to prevent necrosis. Transformed roots also produced callus in the presence of 0.001–1.0% (w/v) of either commercial grade or purified Pluronic. The biotechnological implications of these results are discussed in relation to the potential value of non-ionic surfactants as growth-stimulating additives to plant culture media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036093
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