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  • 1
    ISSN: 1432-0983
    Keywords: Chlamydomonas reinhardtii ; Flagellar regeneration ; ts Mutants ; uni Linkage group
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated a mutant of Chlamydomonas reinhardtii that is both temperature sensitive for viability and temperature sensitive for flagellar regeneration. The mutation (designated tnr1, for temperature-sensitive nonregenerator) has been genetically mapped to a position near uni1 on the uni linkage group (ULG), an unusual genetically circular linkage group consisting primarily of mutations affecting flagellar assembly or function. tnr1 is the first essential gene identified on this linkage group, and is one of the few essential genes affecting flagellar function identified to date. We also find that tnr1 cells are not defective for induction of new tubulin transcripts or protein synthesis during flagellar regeneration at the nonpermissive temperature, and that at least a portion of the unassembled pool of flagellar proteins in mutant cells is assembly-competent at the nonpermissive temperature.
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  • 2
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Restriction fragment length polymorphisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A new field isolate of the unicellular green algaChlamydomonas reinhardtii with useful properties for restriction fragment length polymorphism mapping is described in this report. The isolate, S1-D2 (mating type-), was the only strain found among 24Chlamydomonas isolates taken from many locations which was interfertile with laboratory strains ofC. reinhardtii. It mates at high efficiency, giving tetrads with excellent viability. Using cloned probes for both nuclear and chloroplast genes, we have found numerous restriction fragment length polymorphisms between Sl-D2 and laboratory strains ofC. reinhardtii.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 16 (1989), S. 129-129 
    ISSN: 1432-0983
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Drug resistance ; Pleiotropy ; Dominance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three independent pleiotropic drug-resistance (pdr) mutants were isolated by selecting for resistance to the anti-microtubule herbicides amiprophos-methyl (APM) and oryzalin (ORY). These three mutants and a previously isolated mutant, ani1 (anisomycin resistance), were semi-dominant in heterozygous diploids, and they displayed varying degrees of resistance to structurally and functionally unrelated inhibitors such as cycloheximide, cryptopleurine, emetine, atrazine, and nonidet P-40. Linkage analysis and genetic mapping suggested that three of the four mutants, including ani1, define a single locus, here named pdr1. The fourth mutant defined a new locus, pdr2, which is located on the left arm of linkage group VI. One pdr1 mutant exhibited unusual genetic interactions, including enhanced ts-lethality and synergistic increases in drug resistance, when combined with pdr2-1 and with herbicide-resistant alleles of three other genes.
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  • 5
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Pastures are the most common type of vegetation on deforested land in Amazonia. They vary greatly in the ratio of grass to woody-plant cover, including managed pastures (from which woody vegetation is removed by heavy machinery) and the more common shrub- and tree-dominated "degraded"* pastures15 ...
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  • 6
    ISSN: 1573-5028
    Keywords: blue-light photoreceptor ; Chlamydomonas reinhardtii ; DNA photolyase ; DNA repair
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The organization and nucleotide sequence of a gene from Chlamydomonas reinhardtii encoding a member of the DNA photolyase/blue light photoreceptor protein family is reported. A region of over 7 kb encompassing the gene was sequenced. Northern analysis detected a single 4.2 kb mRNA. The gene consists of eight exons and seven introns, and encodes a predicted protein of 867 amino acids. The first 500 amino acids exhibit significant homology with previously sequenced DNA photolyases, showing the closest relationship to mustard (Sinapis alba) photolyase (43% identity). An even higher identity, 49%, is obtained when the Chlamydomonas gene product is compared to the putative blue-light photoreceptor (HY4) from Arabidopsis thaliana. Both the Chlamydomonas and the Arabidopsis proteins differ from the well characterized DNA photolyases in that they contain a carboxyl terminal extension of 367 and 181 amino acids, respectively. However, there is very little homology between the carboxyl terminal domains of the two proteins. A previously isolated Chlamydomonas mutant, phrl, which is deficient in DNA photolyase activity, especially in the nucleus, was shown by RFLP analysis not to be linked to the gene we have isolated. We propose this gene encodes a candidate Chlamydomonas blue light photoreceptor.
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  • 7
    ISSN: 1617-4623
    Keywords: Gene cluster ; Nitrate transport ; Regulatory mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three overlapping clones covering a Chlarnydomonas reinhardtii genomic region of about 32 kb appear to contain five genes potentially involved in nitrate assimilation in addition to the nitrate reductase structural locus nit-1. These new loci produced transcripts of 2.8, 2.2, 1.8 and 1.7 kb in nitrate-induced wild-type cells that, like the 3.4 kb transcript of nit-1, were undetectable in cells grown in ammonium. In addition, in a mutant defective at the regulatory locus, nit-2 for nitrate assimilation, which does not express the nit-1 gene transcript, accumulation of the four other transcripts was also blocked. They have been named nar (nitrate assimilation related) genes. The nar-1 and nar-2 loci are transcribed in the same orientation as nit-1. The nar-3 and nar-4 loci are transcribed divergently from nit-1. DNA and RNA sequences from both nar-3 and nar-4 cross-hybridized with each other indicating that they share similar sequences. Four nitrate assimilation-deficient mutants (C2, D2, F6 and G1) were characterized. These mutants lack nar transcripts and have major deletions and/or rearrangements in the nar gene cluster. In contrast to other nitrate reductase-deficient mutants and to wild type, deletion mutants and the regulatory mutant nit-2 were incapable of accumulating intracellular nitrate. Two of the mutants in which expression of all of the nar loci did not occur, C2 and D2, grew in nitrite medium and showed wild-type levels of both nitrite uptake and nitrite reductase activities. Thus the nar loci cannot be required for nitrite assimilation. Mutants F6 and G1 were unable to grow in either nitrite- or nitrate-containing medium, and lacked nitrate reductase, nitrite reductase, nitrate uptake and nitrite uptake activities. The inability to assimilate nitrite co-segregated with nit-1 in crosses between these mutants and wild type. These results indicate that a complex gene cluster responsible for the assimilation of nitrate has been identified in C. reinhardtii, and that, in addition, at least one locus necessary for nitrite assimilation is genetically linked to this cluster.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 38 (1998), S. 681-687 
    ISSN: 1573-5028
    Keywords: transposable element ; Tcr3 ; nitrate assimilation ; NIT8 ; Chlamydomonas reinhardtii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new transposable element, Tcr3, was identified in the unicellular green alga Chlamydomonas reinhardtii. The Tcr3 element contained imperfect terminal inverted repeat sequences of 56 bp and created a 2 bp target site duplication upon insertion. Insertion of Tcr3 into the 3′-untranslated region of the NIT8 gene, which is essential for nitrate assimilation, prevented expression of the gene. Excision of the Tcr3 element correlated with reversion of the mutant phenotype and left behind a 3 bp footprint. Tcr3 was found in all Chlamydomonas isolates tested and should prove to be useful for transposon-tagging experiments in Chlamydomonas.
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  • 9
    ISSN: 1572-9729
    Keywords: clay ; drainage class ; geographic information systems ; Histosols ; Maine ; Podzols ; soil carbon ; soil maps ; soil organic matter ; Spodosols ; SSURGO ; STATSGO ; temperate forests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Most estimates of regional and global soil carbon stocks are based on extrapolations of mean soil C contents for broad categories of soil or vegetation types. Uncertainties exist in both the estimates of mean soil C contents and the area over which each mean should be extrapolated. Geographic information systems now permit spatially referenced estimates of soil C at finer scales of resolution than were previously practical. We compared estimates of total soil C stocks of the state of Maine using three methods: (1) multiplying the area of the state by published means of soil C for temperate forests and for Spodosols; (2) calculating areas of inclusions of soil taxa in the 1:5,000,000 FAO/UNESCO Soils Map of the World and multiplying those areas by selected mean carbon contents; and (3) calculating soil C for each soil series and map unit in the 1:250,000 State Soil Geographic Data Base (STATSGO) and summing these estimates for the entire state. The STATSGO estimate of total soil C was between 23% and 49% higher than the common coarse scale extrapolations, primarily because STATSGO included data on Histosols, which cover less than 5% of the area of the state, but which constitute over one-third of the soil C. Spodosols cover about 65% of the state, but contribute less than 39% of the soil C. Estimates of total soil C in Maine based on the FAO map agreed within 8% of the STATSGO estimate for one possible matching of FAO soil taxa with data on soil C, but another plausible matching overestimated soil C stocks. We also compared estimates from the 1:250,000 STATSGO database and from the 1:20,000 Soil Survey Geographic Data Base (SSURGO) for a 7.5 minute quadrangle within the state. SSURGO indicated 13% less total soil C than did STATSGO, largely because the attribute data on depths of soil horizons in SSURGO are more specific for this locality. Despite localized differences, the STATSGO database offers promise of scaling up county soil survey data to regional scales because it includes attribute data and estimates of areal coverage of C-rich inclusions within map units. The spatially referenced data also permit examination of covariation of soil C stocks with soil properties thought to affect stabilization of soil C. Clay content was a poor predictor of soil C in Maine, but drainage class covaried significantly with soil C across the state.
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  • 10
    Publication Date: 1993-06-01
    Print ISSN: 0168-2563
    Electronic ISSN: 1573-515X
    Topics: Chemistry and Pharmacology , Geosciences
    Published by Springer
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