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  • 1
    Publication Date: 2024-03-06
    Description: The present dataset contains the source data for Figure 2B of Tentner et al. (2012). The data shows the percentage of cultured cell-populations that stained positively and/or negatively for apoptotic markers cleaved caspase-3 and cleaved PARP, following DNA damage treatments induced by various doses of doxorubicin (0, 2 and 10 µmole/L) in the presence (100 ng/mL) or absence (0 ng/mL) of TNF-alpha co-treatment. For the six treatment conditions investigated, cell counts were made by flow cytometry at times 6, 12, 24, and 48 h following treatment; CULTURE DETAILS: U2OS cells were obtained from ATCC were maintained at 21% oxygen and 5% CO2 in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum, penicillin, streptomycin, 2mM L-glutamine, and used within 15–20 passages. The first thymidine block was released by washing the plates three times with PBS, and incubating them in fresh thymidine-free media for 12 h. A second thymidine block was then performed by re-addition of thymidine to 2.5 mM followed by incubation for an additional 18 h. Media was aspirated, plates were washed 3 with PBS, and replaced with fresh media in the presence or absence of 10 mM aphidicolin; ANALYSIS DETAILS: See supplementary journal publication; RESULT: The authors of the supplementary journal publication conclude that TNF enhances dose-dependent cell death following doxorubicin-induced DNA damage with minimal affect on dose-dependent cell-cycle arrest.
    Keywords: 775607_Experiment_Culture_Treatment_a; 775607_Experiment_Culture_Treatment_b; 775607_Experiment_Culture_Treatment_c; 775607_Experiment_Culture_Treatment_d; 775607_Experiment_Culture_Treatment_e; 775607_Experiment_Culture_Treatment_f; Cell counts, percent of total; Event label; Flow cytometry; Marker: apoptosis, cleaved caspase-3; Marker: apoptosis, cleaved PARP; Treatment: chemical concentration, of doxorubicin; Treatment: chemical concentration, of TNF alpha; Treatment: time after
    Type: Dataset
    Format: text/tab-separated-values, 3744 data points
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  • 2
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    The @journal of product & brand management 9 (2000), S. 6-20 
    ISSN: 1061-0421
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: Recent interest in the Internet as a medium for commerce has raised questions about the usefulness of branding on the World Wide Web. Examines whether consumers use brands as sources of information when shopping on the Internet. Applying theory from the economics of information, predicts that recent adopters of the Internet will be less proficient at searching for product information and will rely more on brands. As they gather more experience on the Internet, their search proficiency should rise and their brand reliance should fall. These hypotheses are tested and confirmed using usage and opinion survey data from the Internet community. The results suggest that branding can facilitate consumers' acceptance of electronic commerce.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Autonomous robots 3 (1996), S. 213-232 
    ISSN: 1573-7527
    Keywords: underwater vehicle control ; underwater manipulator hydrodynamics ; underwater manipulator modeling ; underwater manipulator control ; coordinated control ; vehicle and manipulator control ; underwater vehicle experiments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The addition of manipulators to small autonomous underwater vehicles (AUVs) can pose significant control challenges due to hydrodynamic interactions between the arm and the vehicle. Experiments conducted at the Monterey Bay Aquarium Research Institute (MBARI) using the OTTER vehicle have shown that dynamical interactions between an arm and a vehicle can be very significant. For the experiments reported in this paper, a single-link “arm” was mounted on OTTER. Tests showed that for 90-degree, two-second repetitive slews of the arm, the vehicle would move as much as 18 degrees in roll and 14 degrees in yaw when no vehicle control was applied. Using a new, highly accurate model of the arm/vehicle hydrodynamic interaction forces, which was developed as part of this research, a coordinated arm/vehicle control strategy was implemented. Under this model-based approach, interaction forces acting on the vehicle due to arm motion were predicted and fed into the vehicle controller. Using this method, station-keeping capability was greatly enhanced. Errors at the manipulator end point were reduced by over a factor of six when compared to results when no control was applied to the vehicle and by a factor of 2.5 when compared to results from a standard independent arm and vehicle feedback control approach. Using the coordinated-control strategy, arm end-point settling times were reduced by a factor three when compared to those obtained with arm and vehicle feedback control alone. These dramatic performance improvements were obtained with only a five-percent increase in total applied thrust.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Autonomous robots 3 (1996), S. 297-320 
    ISSN: 1573-7527
    Keywords: autonomous underwater robot ; task-level control ; control/software architecture ; vision-sensing system ; coordinated arm/vehicle control ; 3D graphical user interfaces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Recent advances in sensing and intelligent control technologies open a whole new dimension in underwater autonomy. However, before truly-capable, autonomous underwater robots can be created for subsea intervention and exploration, many research issues must be first investigated and developed experimentally on testbed platforms. OTTER is an underwater robot designed to be used as a testbed for autonomous technologies. Both OTTER's hardware and software systems are configured to support simultaneous development and testing of different concepts for underwater robotic by independent researchers. A general control-software framework enables common access to all subsystems and avoids the duplication of basic robotic functionality jointly required by all projects. Additionally, the new autonomous technologies enabled by the results of individual research are mutually compatible and can be easily integrated into a single robotic system. Examples of new technologies demonstrated on the OTTER underwater robot include control from a real-time vision-sensing system, coordinated arm/vehicle control, and control from 3D graphical user interfaces.
    Type of Medium: Electronic Resource
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  • 5
    Publication Date: 2019
    Print ISSN: 1434-5161
    Electronic ISSN: 1435-232X
    Topics: Biology , Medicine
    Published by Springer Nature
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  • 6
  • 7
    Publication Date: 2012-03-06
    Description: Analytical Chemistry DOI: 10.1021/ac202637n
    Print ISSN: 0003-2700
    Electronic ISSN: 1520-6882
    Topics: Chemistry and Pharmacology
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  • 8
    Publication Date: 2019
    Description: While the impact of absolute extreme temperatures on human health has been amply studied, far less attention has been given to relative temperature extremes, that is, events that are highly unusual for the time of year but not necessarily extreme relative to a location’s overall climate. In this research, we use a recently defined extreme temperature event metric to define absolute extreme heat events (EHE) and extreme cold events (ECE) using absolute thresholds, and relative extreme heat events (REHE) and relative extreme cold events (RECE) using relative thresholds. All-cause mortality outcomes using a distributed lag nonlinear model are evaluated for the largest 51 metropolitan areas in the US for the period 1975–2010. Both the immediate impacts and the cumulative 20-day impacts are assessed for each of the extreme temperature event types. The 51 metropolitan areas were then grouped into 8 regions for meta-analysis. For heat events, the greatest mortality increases occur with a 0-day lag, with the subsequent days showing below-expected mortality (harvesting) that decreases the overall cumulative impact. For EHE, increases in mortality are still statistically significant when examined over 20 days. For REHE, it appears as though the day-0 increase in mortality is short-term displacement. For cold events, both relative and absolute, there is little mortality increase on day 0, but the impacts increase on subsequent days. Cumulative impacts are statistically significant at more than half of the stations for both ECE and RECE. The response to absolute ECE is strongest, but is also significant when using RECE across several southern locations, suggesting that there may be a lack of acclimatization, increasing mortality in relative cold events both early and late in winter.
    Print ISSN: 1661-7827
    Electronic ISSN: 1660-4601
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Published by MDPI
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  • 9
    Publication Date: 2019
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
    Published by Springer Nature
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  • 10
    Publication Date: 2018-08-22
    Description: Remote Sensing, Vol. 10, Pages 1326: How Long should the MISR Record Be when Evaluating Aerosol Optical Depth Climatology in Climate Models? Remote Sensing doi: 10.3390/rs10091326 Authors: Huikyo Lee Michael J. Garay Olga V. Kalashnikova Yan Yu Peter B. Gibson This study used the nearly continuous 17-year observation record from the Multi- angle Imaging SpectroRadiometer (MISR) instrument on the National Aeronautics and Space Administration (NASA) Terra Earth Observing System satellite to determine which temporal subsets are long enough to define statistically stable speciated aerosol optical depth (AOD) climatologies (i.e., AOD by particle types) for purposes of climate model evaluation. A random subsampling of seasonally averaged total and speciated AOD retrievals was performed to quantitatively assess the statistical stability in the climatology, represented by the minimum record length required for the standard deviation of the subsampled mean AODs to be less than a certain threshold. Our results indicate that the multi-year mean speciated AOD from MISR is stable on a global scale; however, there is substantial regional variability in the assessed stability. This implies that in some regions, even 17 years may not provide a long enough sample to define regional mean total and speciated AOD climatologies. We further investigated the agreement between the statistical stability of total AOD retrievals from MISR and the Moderate Resolution Imaging Spectroradiometer (MODIS), also on the NASA Terra satellite. The difference in the minimum record lengths between MISR and MODIS climatologies of total AOD is less than three years for most of the globe, with the exception of certain regions. Finally, we compared the seasonal cycles in the MISR total and speciated AODs with those simulated by three global chemistry transport models in the regions of climatologically stable speciated AODs. We found that only one model reproduced the observed seasonal cycles of the total and non-absorbing AODs over East China, but the seasonal cycles in total and dust AODs in all models are similar to those from MISR in Western Africa. This work provides a new method for considering the statistical stability of satellite-derived climatologies and illustrates the value of MISR’s speciated AOD data record for evaluating aerosols in global models.
    Electronic ISSN: 2072-4292
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Published by MDPI Publishing
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