ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Appropriate culture conditions cause bone marrow stem cells to differentiate intomultilineage cells such as adipocytes, chondrocytes, and osteoblasts. One key factor that regulatesintercellular signaling and cell differentiation is the extracellular matrix microenvironment. Thecomposition of the extracellular matrix influences cellular functions. In the present study, weinvestigated the effects of a microenvironment comprising a three-dimensional apatite-fiber scaffold(AFS) that has two kinds of pores (micro- and macro pores) on proliferation and subsequentdifferentiation of bone marrow stem cells. Morphologic observation revealed that osteoblastic cells inthe AFS were distributed primarily in the same location on the fibrous scaffold and formed bridgeswithin micro- and macro pores. We used molecular approaches to evaluate cell proliferation anddifferentiation in detail. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showedthat culturing bone marrow cells on AFS increases expression of osteocalcin (OC) mRNA comparedwith culture in a dish. Furthermore, cells cultured in AFS expressed type X collagen (Col X), which isa marker of hypertrophic cartilage. These data suggest that the three-dimensional microenvironmentof AFS facilitates cell proliferation and differentiation, and promotes endochondral ossification ofbone marrow cells
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.1075.pdf
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