ISSN:
1662-8985
Quelle:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Thema:
Maschinenbau
Notizen:
Mussel foot protein is widely known for candidate of strong adhesive materials. Sixtimes fp-1 decapeptide repeats and fp-5 are chosen among the various type of foot protein and fusedinto (MAP) fp-151. Through various cell-adhesion analyses, we previously demonstrated that fp-151 has the potential to be used as a cell or tissue bioadhesive. In the present study, to improve thecell-adhesion properties of fp-151, it was designed a new cell-adhesive protein, fp-151-RGD, whichis a fusion with the GRGDSP residues, a RGD peptide sequence that has previously been identifiedat the cell-attachment site of fibronectin, at the C-terminus of fp-151. Also, recombinant fp-151-RGD maintained the advantages associated with fp-151, such as a high production yield inEscherichia coli and simple purification, it showed superior spreading ability, which is importantfor cell proliferation under serum-free conditions, as well as better cell-adhesion ability comparedwith other commercially produced cell-adhesion materials such as poly-L-lysine (PLL) and thenaturally extracted MAP mixture Cell-Tak. The excellent adhesion and spreading abilities of fp-151-RGD might be due to the fact that it utilizes three types of cell-binding mechanisms: DOPAadhesion of Cell-Tak, cationic binding force of PLL, and RGD sequence-mediated adhesion offibronectin. Therefore, the new recombinant fp-151-RGD is suitable for use as a cell-adhesionmaterial in cell culture or tissue engineering, and in any other area where efficient cell adhesion isrequired
Materialart:
Digitale Medien
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/41/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.47-50.861.pdf
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