ISSN:
1573-5087
Keywords:
benzyladenine
;
cytokinin
;
2,4-dichlorophenoxyacetic acid
;
embryogenesis
;
organogenesis
;
thidiazuron
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract A prerequisite for most transformation systems is an efficient and reliable method to regenerate phenotypically normal plants. Immature embryos or cotyledons were cultured at three developmental stages (stage 1, 2 and 3, PF=3, 30–60, and 100, respectively) from two unrelated apricot genotypes, ‘Zard’ and ‘NJA82’. Explants were cultured on MS media supplemented with either BA or TDZ at four concentrations (0, 0.5, 5.0 or 20 μM) and 2,4-D at 0 or 1 μM. Stage 1 embryos cultured on MS medium without growth regulators formed embryoid-like structures. Shoot primordia induction was greatest with stage 2 cotyledons on media containing 5–20 μM TDZ and 1 μM 2,4-D, although shoot morphology was abnormal, especially with the highest level of TDZ. In another factorial experiment, stage 2 cotyledons were cultured on media containing TDZ (0, 5, 7.5, 10, 15 or 20 μM) in combination with either no auxin, 1 μM 2,4-D, 1 μM IBA, or 5 μMIBA. Regeneration percentages of 80% or more were observed on media containing 1–5 μM IBA and 5–10 μM TDZ. The medium containing 5 μM IBA and no TDZ exhibited the highest frequency of phenotypically normal plantlet regeneration.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00024493
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