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  • 1
    Electronic Resource
    Electronic Resource
    Inheritance of high palmitic acid content in the sunflower mutant CAS-12 and its relationship with high oleic content (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: CAS-12 is a sunflower mutant with increased levels of palmitic (C16: 0 = 30%) and oleic (C18: 1 = 55%) acids in its seed oil, hence it has a reduced linoleic acid content (C18: 2 〈 5%). This study was conducted to determine the inheritance of high C16: 0 content and its relationship with high C18: 1 content in CAS-12. Reciprocal crosses involving CAS-12, CAS-5 (high C16: 0 content), HAOL-9 (high C18: 1 content) and HA-89 (standard fatty acid profile) were made. The F1, F2 and BC1F1 generations were obtained. The genetic control of the high C16: 0 trait in CAS-12 was partially recessive and gametophytic. In all cases, this character segregated in the ratio 19: 38: 7 (low: intermediate: high C16: 0 content) in the F2 generation. These results, together with the lack of segregation for C16: 0 content in crosses between CAS-12 and CAS-5, indicated that the genetic control of the high C16: 0 trait in CAS-12 was similar to that in CAS-5 in being controlled by partially recessive alleles (p1, p2, and p3) at three loci. Crosses between HA-89 and CAS-12, and HAOL-9 and CAS-5 (segregating for C16: 0 and C18: 1) demonstrated that the high C16: 0 and the high C18: 1 traits were independently inherited. However, C18: 1 segregation in these crosses exhibited reversal of dominance. Apparently, the low C18: 1 parental lines carried modifier genes causing the deviation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2002.00655.x
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  • 2
    Electronic Resource
    Electronic Resource
    One-Step Lipid Extraction and Fatty Acid Methyl Esters Preparation from Fresh Plant Tissues
    Amsterdam : Elsevier
    Analytical Biochemistry 211 (1993), S. 139-143 
    Details
    ISSN: 0003-2697
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/abio.1993.1244
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  • 3
    Electronic Resource
    Electronic Resource
    Oleate desaturation in seeds of two genotypes of sunflower
    Amsterdam : Elsevier
    Phytochemistry 28 (1989), S. 2593-2595 
    Details
    ISSN: 0031-9422
    Keywords: Compositae ; Helianthus annuus ; high oleic acid mutant. ; oleate desaturation ; sunflower, seeds
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0031-9422(00)98046-4
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  • 4
    Electronic Resource
    Electronic Resource
    Lipid characterization in seeds of a high oleic acid sunflower mutant
    Amsterdam : Elsevier
    Phytochemistry 28 (1989), S. 2597-2600 
    Details
    ISSN: 0031-9422
    Keywords: Compositae ; Helianthus annuus ; fatty acid composition ; high oleic acid mutant. ; lipids ; seeds ; sunflower
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0031-9422(00)98047-6
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  • 5
    Electronic Resource
    Electronic Resource
    In vitro oleate desaturase in developing sunflower seeds
    Amsterdam : Elsevier
    Phytochemistry 30 (1991), S. 2127-2130 
    Details
    ISSN: 0031-9422
    Keywords: Compositae ; Helianthus annuus ; high oleic mutant ; microsomes ; oleate desaturase ; seeds ; sunflower ; temperature control.
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0031-9422(91)83599-G
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  • 6
    Electronic Resource
    Electronic Resource
    Examination of Phycomyces blakesleeanus for nitrate reductase as a possible blue light photoreceptor
    Amsterdam : Elsevier
    Plant Science 40 (1985), S. 173-177 
    Details
    ISSN: 0168-9452
    Keywords: Phycomyces ; blue light photoreceptor ; nitrate reductase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(85)90199-2
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  • 7
    Electronic Resource
    Electronic Resource
    New sunflower mutants with altered seed fatty acid composition
    Amsterdam : Elsevier
    Progress in Lipid Research 33 (1994), S. 147-154 
    Details
    ISSN: 0163-7827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0163-7827(94)90017-5
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  • 8
    Electronic Resource
    Electronic Resource
    Temperature regulation of oleate desaturase in sunflower (Helianthus annuus L.) seeds (1992)
    Springer
    Planta 186 (1992), S. 461-465 
    Details
    ISSN: 1432-2048
    Keywords: Helianthus ; Oleate desaturase ; Fatty acid unsaturation ; Membrane fatty acids ; Seed (fatty acids)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of temperature on oleate desaturation in developing sunflower (Helianthus annuus L.) seeds has been examined. When seeds from plants grown at low (20/10° C, day/night) temperature were transferred for 24 h to 10° C, an increase in the linoleate/oleate ratio in phosphatidylcholine and triacylglycerol was observed, but not when transfer was to 20 or 30° C. The same effect was observed in triacylglycerol, phosphatidylcholine and phosphatidylethanolamine in the newly synthesized lipids after in-vivo incubation with [1-14C]oleate at 10° C. The microsomal oleoyl phosphatidylcholine desaturase (ODS) activity of the seeds maintained at 10† C was also enhanced. The stimulation was observed after only 3 h in plants grown at high temperature (30/20° C). This effect was inhibited by cycloheximide, implying that the low-temperature stimulation of the ODS activity was caused by the synthesis of new enzyme. As a consequence, seeds from plants grown at low temperature had higher ODS activities and linoleate contents than those grown at high temperature. The microsomal ODS activity of seeds from plants grown at low temperature was dependent on incubation temperature and showed a maximum at 20° C. By contrast, this activity was almost temperature-insensitive in seeds from plants grown at high temperature. These results could explain how temperature regulates the fatty-acid composition in sunflower-seed lipids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195328
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  • 9
    Electronic Resource
    Electronic Resource
    Epistatic interaction among loci controlling the palmitic and the stearic acid levels in the seed oil of sunflower (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 105-111 
    Details
    ISSN: 1432-2242
    Keywords: Key words Sunflower ; Helianthus annuus ; High palmitic acid ; High stearic acid ; Epistatic interaction ; Inheritance ; Oil quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Two sunflower (Helianthus annuus L.) mutants with high concentrations of saturated fatty acids in their seed oil have been identified and studied extensively. The mutant line CAS-5 has high concentrations of palmitic acid (C16:0) (〉25% compared with 7% in standard sunflower seed oil) and low-C18:0 values (3%). CAS-3 is characterized by its high levels of stearic acid (C18:0) (〉22% compared with 4% in standard sunflower seed oil) and a low-C16:0 content (5%). CAS-5 also possesses elevated levels of palmitoleic acid (C16:1) (〉5%), which is absent in standard sunflower seed oil. The objective of this study was to determine the relationships between the loci controlling the high-C16:0 and the high-C18:0 traits in these mutants. Plants of both mutants were reciprocally crossed. Gas chromatographic analyses of fatty acids from the seed oil of F1, F2, F3 and the BC1F1 to CAS-5 generations indicated that the loci controlling the high-C16:0 trait exerted an epistatic effect over the loci responsible for the high-C18:0 character. As a result, the phenotypic combination containing both the high-C16:0 levels of CAS-5 and the high-C18:0 levels of CAS-3 was not possible. However, phenotypes with a saturated fatty acid content of 44% (34.5% C16:0+9.5% C18:0) were identified in the F3 generation. These are the highest saturated (C16:0 and C18:0) levels reported so far in sunflower seed oil. When F3 C16:0 segregating generations in both a high- and a low-C18:0 background were compared, the high-C16:1 levels were not expressed as expected in the high-C18:0 background (CAS-3 background). In this case, the C16:1 content decreased to values below 1.5%, compared with 〉5% in a low-C18:0 background. As the stearoyl-ACP desaturase has been reported to catalyze the desaturation from C16:0-ACP to C16:1-ACP, these results suggested that a decrease in its activity was involved in the accumulation of C18:0 in the high-C18:0 mutant CAS-3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050015
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  • 10
    Electronic Resource
    Electronic Resource
    Oleate from triacylglycerols is desaturated in cold-induced developing sunflower (Helianthus annuus L.) seeds (1994)
    Springer
    Planta 193 (1994), S. 473-477 
    Details
    ISSN: 1432-2048
    Keywords: Helianthus ; Microsome ; Oil body ; Oleate desaturation ; Seed (fatty acids) ; Triacylglycerol metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For the first time, an active fatty-acid metabolism is indicated for triacylglycerols (TAG) of developing sunflower (Helianthus annuus L.) seeds. When the developing seeds were transferred to low temperature, the total amount of oleate found in TAG decreased as that of linoleate increased, while the contents of total lipids and TAG remained unchanged. These results suggest that oleate from TAG was used for desaturation. This occurred first in microsomal TAG, but after a long cold period it was observed mainly in the oil-body fraction. Thesn-2 position of TAG was preferentially enriched in linoleate. Apparently, more linoleate than necesary for the maintenance of membrane fluidity was synthesized at the expense of TAG oleate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02411550
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