Abstract
For the first time, an active fatty-acid metabolism is indicated for triacylglycerols (TAG) of developing sunflower (Helianthus annuus L.) seeds. When the developing seeds were transferred to low temperature, the total amount of oleate found in TAG decreased as that of linoleate increased, while the contents of total lipids and TAG remained unchanged. These results suggest that oleate from TAG was used for desaturation. This occurred first in microsomal TAG, but after a long cold period it was observed mainly in the oil-body fraction. Thesn-2 position of TAG was preferentially enriched in linoleate. Apparently, more linoleate than necesary for the maintenance of membrane fluidity was synthesized at the expense of TAG oleate.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- DAF:
-
days after flowering
- ODS:
-
oleoyl phosphatidylcholine desaturase
- PC:
-
phosphatidylcholine
- TAG:
-
triacylglycerol(s) 16:0
- P:
-
palmitate; 18:0
- S:
-
stearate; 18:1
- O:
-
oleate 18:2
- L:
-
linoleate
References
Browse, J., Slack, C.R. (1983) The effects of temperature and oxygen on the rates of fatty acid synthesis and oleate desaturation in safflower (Carthamus tinctorius) seed. Biochim. Biophys. Acta753, 145–152.
Browse, J., Somerville, C. (1991) Glycerolipid synthesis: Biochemistry and regulation. Annu. Rev. Plant Physiol. Plant Mol. Biol.42, 467–506.
Carelli, A.A., Cert, A. (1993) Comparative study of the determination of triacylglycerol in vegetable oils using chromatographic techniques. J. Chromatogr.630, 213–222
Frey-Wyssling, A., Grieshaber, E., Mühlethaler, K. (1963) Origin of spherosomes in plant cells. J. Ultrastruct Res.8, 506–516
Garcés, R., Mancha, M. (1989) Oleate desaturation in seeds of two genotypes of sunflower. Phytochemistry28, 2593–2595
Garcés, R., Mancha, M. (1991) In vitro oleate desaturase in developing sunflower seeds. Phytochemistry30, 2127–2130
Garcés, R., Sarmiento, C., Mancha, M. (1992) Temperature regulation of oleate desaturase in sunflower (Helianthus annuus L.) seeds. Planta186, 461–465
Hara, A., Radin, N.S. (1978) Lipid extraction of tissues with a low-toxicity solvent. Anal. Biochem.90, 420–426.
Mancha, M. (1975) Estudio de los diglicéridos naturales de la pulpa de la aceituna. Grasas Aceites26, 347–352
Mancha, M., Sanchez, J. (1981) Incorporation of free fatty acids into acylthioesters and lipids of developing sunflower seeds. Phytochemistry20, 2139–2142
Slack, C.R., Roughan, P.G., Balasingham, N. (1978) Labelling of glycerolipids in the cotyledons of developing oilseeds by [1-14C] acetate and [2-3H] glycerol Biochem. J.170, 421–433
Stymne, S., Appelqvist, L.Å (1978) The biosynthesis of linoleate from oleoyl-CoA via oleoyl phosphatidylcholine in microsomes of developing safflower seeds. Eur. J. Biochem.90, 223–229
Stymne, S., Stobart, A.K. (1984) Evidence for the reversibility of the acyl-CoA: lysophosphatidylcholine acyltransferase in microsomal preparations from developing safflower (Carthamus tinctorius L.) cotyledons and rat liver. Biochem. J.223, 305–314
Vander Wal, R.J. (1960) Calculation of the distribution of the saturated and unsaturated acyl groups in fats, from pancreatic lipase hydrolysis data. J. Am. Oil Chem. Soc.37, 18–20
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Garcés, R., Sarmiento, C. & Mancha, M. Oleate from triacylglycerols is desaturated in cold-induced developing sunflower (Helianthus annuus L.) seeds. Planta 193, 473–477 (1994). https://doi.org/10.1007/BF02411550
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02411550