ISSN:
1432-0983
Keywords:
Key wordsSaccharomyces cerevisiae
;
Protein transport
;
Golgi apparatus
;
Brefeldin A
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The fungal metabolite Brefeldin A (BFA) disrupts the Golgi apparatus and its incoming protein flux. We developed a genetic approach to identify yeast proteins involved in the protein transport step that BFA blocks. The BFR2 gene (YDR299W) was thus isolated as a high-copy suppressor of the growth defects induced by BFA in a sensitive strain of Saccharomyces cerevisiae. Although BFR2 over-expression did not cause a secretory block or slow-down, it partially suppressed the growth defect of four mutants blocked at the step of budding or docking of small vesicles en route to the Golgi (sec13-1, sec16-2, sec23-1, ypt1-1). The essential BFR2 gene was predicted to encode an extremely hydrophilic product containing two short regions with potential coiled-coils, one of which corresponds to a cluster of acidic residues.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050304
Permalink