Publication Date:
1988-09-30
Description:
In most studies of synaptic currents in mammalian central neurons, preparations have been used in which synaptic currents are recorded at some distance from the synapse itself. This procedure introduces problems in interpretation of the kinetics and voltage-dependent properties of the synaptic current. These problems have now been overcome by the development of a preparation in which presynaptic vesicle-containing boutons have been coisolated with the soma of individual neurons, thus providing the opportunity to study synaptic currents under conditions of both adequate voltage control and internal ionic perfusion. Spontaneous synaptic currents mediated by gamma-aminobutyric acid and excitatory amino acids were recorded from neurons isolated from a mammalian medial solitary tract nucleus. Calcium- and depolarization-dependent spontaneous currents of several to hundreds of picoamperes occurred with rapid rise times of 0.8 to 3 milliseconds and decays at least ten times as long.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Drewe, J A -- Childs, G V -- Kunze, D L -- HL36840/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1988 Sep 30;241(4874):1810-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston 77550.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2459774" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Calcium/physiology
;
Glutamates/physiology
;
Guinea Pigs
;
In Vitro Techniques
;
Ion Channels/physiology
;
Membrane Potentials
;
Neurons/*physiology
;
Potassium/physiology
;
Synapses/*physiology
;
*Synaptic Transmission
;
gamma-Aminobutyric Acid/physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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