Publication Date:
1989-10-13
Description:
Cellular metabolism is affected by many factors in a cell's environment. Given a sufficiently sensitive method for measuring cellular metabolic rates, it should be possible to detect a wide variety of chemical and physical stimuli. A biosensor has been constructed in which living cells are confined to a flow chamber in which a potentiometric sensor continually measures the rate of production of acidic metabolites. Exploratory studies demonstrate several applications of the device in basic science and technology.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Parce, J W -- Owicki, J C -- Kercso, K M -- Sigal, G B -- Wada, H G -- Muir, V C -- Bousse, L J -- Ross, K L -- Sikic, B I -- McConnell, H M -- R01-CA-4217/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1989 Oct 13;246(4927):243-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Molecular Devices Corporation, Menlo Park, CA 94025.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2799384" target="_blank"〉PubMed〈/a〉
Keywords:
*Biosensing Techniques
;
Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology
;
Cells/*metabolism
;
Cells, Cultured/drug effects/metabolism
;
Epidermal Growth Factor/pharmacology
;
Flow Cytometry
;
Humans
;
Oxygen Consumption
;
Silicon
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink