Publication Date:
2013-01-01
Description:
Background.gcrRgene acts as a negative regulator related to sucrose-dependent adherence inS. mutans. It is constructive to test the potential capacity of mutans withgcrRgene deficient in bacteria replacement therapy.Methods. In this study, we constructed the mutant by homologous recombination. The morphological characteristics of biofilms were analyzed by confocal laser scanning microscopy.S. mutansUA159 and the mutant MS-gcrR-def were inoculated, respectively, or together for competitive testing in vitro and in rat model.Results. Adhesion assay showed that the adhesion ability of the mutant increased relative to the wild type, especially in the early stage. MS-gcrR-def out-competedS. mutansUA159 in vitro biofilm, and correspondingly coinfection displayed significantly fewer caries in vivo. The former possessed both a lower level of acid production and a stronger colonization potential thanS. mutansUA159.Conclusion. These findings demonstrate that MS-gcrR-def appears to be a good candidate for replacement therapy.
Print ISSN:
2356-6140
Electronic ISSN:
1537-744X
Topics:
Natural Sciences in General
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