ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary High performance liquid chromatography (HPLC) was used to detect and quantify β-exotoxin, a phosphorylated adenine nucleotide derivative produced as an excreted metabolite by several strains of Bacillus thuringiensis. The assay was rapid and quantitative for purified β-exotoxin standards. However, peak height failed to correlate β-exotoxin concentration in crude culture filtrates with biological activity toward house fly larvae. Unrelated compounds (from non-β-exotoxin producers) co-eluted with β-exotoxin, thereby making the technique an unreliable method for toxin detection and quantification.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00510421
Permalink