ISSN:
1432-0983
Keywords:
Key wordsErysiphe graminis
;
Polymerase chain reaction
;
Cytochrome P450
;
Fungicide
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Molecular studies of some micro-organisms are hampered by the difficulty of obtaining sufficient amounts of nucleic acids. A cloning strategy based on PCR has therefore been used to clone the eburicol 14α-demethylase (CYP51) gene of the obligate fungus Erysiphe graminis f. sp. hordei (Egh) using minute amounts of genomic DNA. The CYP51 gene encodes the enzymatic target of a major group of fungicides. Sequencing CYP51 from different Egh isolates revealed the occurrence of two alleles for this gene. An allele-specific PCR assay was developed to detect each CYP51 allele.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050413
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