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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Freshwater biology 50 (2005), S. 0 
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 1. The influence of hydraulic conditions on the spatial distribution of macroinvertebrate assemblages was investigated in three riffles in a perennial Australian river.2. Velocity, depth and variability of substrate roughness were measured at each of 56 macroinvertebrate sampling locations. Complex hydraulic variables (roughness Reynolds number, shear velocity, Froude number) were calculated from combinations of two or three of the directly measured variables. The biological significance of directly measured and complex hydraulic variables was determined by a combination of univariate and multivariate statistical procedures.3. Macroinvertebrate abundance, number of taxa and community composition were significantly different between the identified roughness Reynolds number, Froude number, velocity and shear velocity microhabitats throughout the studied riffles.4. Regression analysis showed macroinvertebrate abundance and number of taxa were negatively related to roughness Reynolds number, shear velocity, velocity and Froude number. Depth was negatively related to abundance. In general, the majority of the macroinvertebrate community preferred the areas of riffles with the lowest near-bed turbulence.5. Roughness Reynolds number explained more of the spatial variation in invertebrate abundance, number of taxa and community composition than the other hydraulic variables, either directly measured or calculated. Of the directly measured variables, velocity had the greatest explanatory power, which was marginally less than roughness Reynolds number and shear velocity.6. This study demonstrated that small-scale differences in hydraulic conditions created by combinations of velocity, depth and substrate roughness have an important role in the spatial distribution of macroinvertebrate assemblages in riffle habitats.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science, Ltd
    Freshwater biology 41 (1999), S. 0 
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 1. Within a few decades of European disturbance in the mid-nineteenth century, river character and behaviour were transformed in Bega catchment on the south coast of New South Wales, Australia. Ecological impacts of geomorphic changes to river structure and function throughout the catchment are assessed.2. At the time of European settlement, many water courses in Bega catchment were discontinuous, with extensive swamps along middle and upper courses. Following a series of direct and indirect human impacts, channels became continuous in the middle and upper parts of the catchment, as extensive valley fills at the base of the escarpment were incised. Along the lowland plain, the channel widened by over 300%, fundamentally altering the relationship between the channel and its adjacent floodplain.3. Geomorphic changes to river structure have modified habitat availability throughout Bega catchment. The impacts have been least pronounced in headwater streams, but have been dramatic along virtually all river courses beyond the base of the escarpment.4. Changes in river structure have been directly related to altered riparian vegetation cover, and vice versa. As a consequence of changes to river structure, bed substrate calibre (and supply volume/rate) has been modified along most streams.5. A series of indirect, secondary impacts have modified habitat viability along river courses. Lateral, longitudinal and vertical linkages within the river system have been altered, affecting the transfer of water, sediment, organic matter, nutrients and other biotic interactions.6. These direct and indirect consequences of geomorphic changes in river structure suggest that ecologists need to adopt a longer-term, catchment-framed view of human disturbance to river ecosystems.7. Effective, sustainable ecological rehabilitation of river courses is dependent on an understanding of geomorphic processes and determination of appropriate river structure at differing positions in catchments.
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  • 3
    ISSN: 1432-2048
    Keywords: Cucurbita (gluconeogenesis) ; Gluconeogenesis ; Metabolic flux control analysis ; Phosphoenol ; pyruvate carboxykinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 3-Mercaptopicolinic acid, a non-competitive inhibitor of phosphoenolpyruvate carboxykinase (EC 4.1.1.19) was used to study the control of gluconeogenesis by this enzyme in germinating marrow (Cucurbita pepo) cotyledons. In vitro, phosphoenolpyruvate carboxykinase was inhibited by 3-mercaptopicolinic acid, with a Ki of 5.9 μM. At 25°C the inhibitor caused an increase in the label incorporated from [2-14C]acetate into CO2, and a decrease in the label incorporated into the insoluble and neutral fractions. Phosphoenolpyruvate carboxykinase had a flux control coefficient for gluconeogenesis (C PEPCK J ) of between 0.7 and 1.0. 3-Mercaptopicolinic acid was a less effective inhibitor of phosphoenolpyruvate carboxykinase at lower temperatures (Ki = 8.6 μM at 17°C, 13.3 μM at 10°C) and had similar effects on the metabolism of [2-14C]acetate by marrow cotyledons when the temperature was reduced to 17°C and 10°C. The control coefficient for this enzyme did not change with temperature, indicating that phosphoenolpyruvate carboxykinase exerts a high degree of control over gluconeogenesis at all temperatures examined.
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  • 4
    ISSN: 1432-2048
    Keywords: Cucurbita (gluconeogenesis) ; Gluconeogenesis ; Metabolic flux control analysis ; Phosphoenol ; pyruvate carboxykinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 3-Mercaptopicolinic acid, a non-competitive inhibitor of phosphoenolpyruvate carboxykinase (EC 4.1.1.19) was used to study the control of gluconeogenesis by this enzyme in germinating marrow (Cucurbita pepo) cotyledons. In vitro, phosphoenolpyruvate carboxykinase was inhibited by 3-mercaptopicolinic acid, with aKi of 5.9 μM. At 25°C the inhibitor caused an increase in the label incorporated from [2-14C]acetate into CO2, and a decrease in the label incorporated into the insoluble and neutral fractions. Phosphoenolpyruvate carboxykinase had a flux control coefficient for gluconeogenesis (C PEPCK J ) of between 0.7 and 1.0. 3-Mercaptopicolinic acid was a less effective inhibitor of phosphoenolpyruvate carboxykinase at lower temperatures (Ki = 8.6 μM at 17°C, 13.3 μM at 10°C) and had similar effects on the metabolism of [2-14C]acetate by marrow cotyledons when the temperature was reduced to 17°C and 10°C. The control coefficient for this enzyme did not change with temperature, indicating that phosphoenolpyruvate carboxykinase exerts a high degree of control over gluconeogenesis at all temperatures examined.
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  • 5
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Neurovascular dysfunction substantially contributes to Alzheimer disease. Here, we show that transcriptional profiling of human brain endothelial cells (BECs) defines a subset of genes whose expression is age-independent but is considerably altered in Alzheimer disease, including the homeobox gene ...
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  • 6
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Focal molecular genetic alteration of the intact mammalian brain will be required to elucidate gene product function in cells comprising synaptic networks. To this end, a somatic mosaic approach has been developed for the mouse whereby a dormant germline transgene is activated by the somatic ...
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 291 (1981), S. 7-7 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR - PhDs normally take longer than three years to complete and their exact purpose is not agreed upon (Nature 22 January, p.217). As an SRC-supported research student, I would like to make a few relevant comments. I have spent several months in my library carrying out the customary literature ...
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  • 8
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Target cell lysis is regulated by natural killer (NK) cell receptors that recognize class I MHC molecules. Here we report the crystal structure of the human immunoglobulin-like NK cell receptor KIR2DL2 in complex with its class I ligand HLA-Cw3 and peptide. KIR binds in a nearly orthogonal ...
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of biomedical science 5 (1998), S. 321-331 
    ISSN: 1423-0127
    Keywords: Natural killer cells ; HLA-E ; CD94 ; NKG2 ; Major histocompatibility complex, class I
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract CD94/NKG2 is a recently described receptor present on natural killer (NK) cells and certain T cells that is composed of the CD94 chain covalently associated with a member of the NKG2 family of molecules. Both chains are glycosylated members of the C-type lectin superfamily. The CD94/NKG2 receptors are functionally heterogenous depending on which NKG2 family member is associated with CD94. Initially, it was thought that CD94/NKG2 receptors recognized a broad array of HLA-A, -B and -C (classical), as well as the nonclassical HLA-G, MHC class I molecules. Instead, recent data have suggested that this receptor is specific for HLA-E complexed with a peptide derived from the signal sequence (residues 3–11) of certain classical MHC class I molecules. Position 2 (residue 4) in the signal sequence derived peptides appears pivotal in determining whether the HLA-E/peptide complex confers resistance to NK-mediated lysis. The potential roles that the CD94/NKG2-HLA-E receptor ligand interaction might play in infection and tumor development are discussed.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Automated software engineering 3 (1996), S. 193-218 
    ISSN: 1573-7535
    Keywords: Software inspection ; CASE ; collaborative work
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract Inspection is widely believed to be the most cost-effective method for detecting defects in documents produced during the software development lifecycle. However, it is by its very nature a labour intensive process. This has led to work on computer support for the process which should increase the efficiency and effectiveness beyond what is currently possible with a solely manual process. In this paper, we first of all describe current approaches to automation of the inspection process. There are four main areas of inspection which have been the target for computer support: document handling, individual preparation, meeting support and metrics collection. We then describe five tools which have been developed to support the inspection process and compare the capabilities of these tools. This is followed by a fuller discussion of the features which could be provided by computer support for inspection and the gains that may be achieved by using such support.
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