ISSN:
1573-5001
Keywords:
Carbon chemical shifts
;
Cytochrome c
;
Secondary structure
;
Heme ring current effect
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Calculations suggest that some carbon chemical shifts in proteinsshould have large ring current shifts (〉1 ppm). We present13C, 15N and 1H assignments forcytochrome c2 from Rhodospirillum rubrum, compare these withshifts for other cytochromes c, and show that the calculated ring currentshifts are similar to experimentally observed shifts, but that there remainsubstantial conformation-dependent shifts of side-chain carbons. Ringcurrent shifts as large as 6 ppm are observed. We show that the ring currenteffects do not seriously affect the Chemical Shift Index method fordelineating secondary structure, but may have an impact on more precisemethods for generating structural constraints.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018394410613
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