ALBERT

Description: SUMMARYA method of including urea into whole grains is described. The whole grains were found to absorb completely a saturated solution of urea.The method gave the different cereals examined (barley, oats, wheat and maize) a homogeneouis distribution of urea and increased the voluntary intake of whole grains by lambs. The evolution of NH3 in the rumen liquor was slightly slower when urea was absorbed into the grains than when it was included as urea crystals. The N content in the urea-treated grain was not changed after 6 months of storage.

Description: SUMMARYThe artificial fibre bag technique was evaluated for assessing the proportions of dietary D. M. and N which disappear in the rumen. The most important factor determining the variability in disappearance from bags incubated together was the sample size in relation to bag size. For incubation of 5 g air dry feed, a bag size of 17 ✗ 9 cm was found to be adequate. There were also variations in substrate disappearance between animals and between days of incubation. It was estimated that three sheep and the measurement of substrate disappearance twice were necessary in order to obtain acceptable repeatability.The technique was found to be satisfactory as a simple and rapid guide for measuring nutrients disappearance in the rumen such as protein degradation and carbohydrate fermentation. It has been used in subsequent work to measure the effect of features of the rumen environment, such as pH and NH3 concentration, on rate of fermentation.

Description: SUMMARYMale and female (Suffolk × (Finnish Landraee × Dorset Horn)) lambs were used in three experiments to examine the replacement of milk by undried and dried fish protein hydrolysates (FPH), a mixture of lard and coconut fat, and partially hydrolysed starch (Protamyl 110). The animals received the experimental diets from 1 week of age in Expts 1 and 2, and from 4 weeks of age in Expt 3. They were given the diets in eight equal feeds (i.e. every 3 h) from an automated teat bar system. The level of feeding was 1·046 MJ/kg0−76/day. Experiments 1 and 2 lasted for 35 days, during which live-weight gain and food conversion ratios were recorded and at the end the digestibility of N, dry matter and starch was determined. Experiment 3 consisted of three small digestibility trials.In Expt 1, the replacement of milk protein with undried FPH and of milk fat with lard plus coconut fat had no significant effects on live-weight gain, food conversion ratio or nutrient digestibility. Somewhat lower gains for the lambs given the FPH than those given milk protein were observed during the first 15 days of the experiment. Apparent digestibility of milk protein and milk fat was 95 and 99% while that of PFH and lard plus coconut fat was 94 and 96% respectively. The replacement of milk fat with lard and coconut fat caused some reduction in live-weight gains and nutrient digestibility. Lactose was completely replaced by protamyl both in diets based on milk protein or FPH with no effect on live-weight gains or food conversion ratio.Apparent digestibility of fish protein was not affected by the drying process. Fat digestibility of diets containing undried or dried FPH ranged from 46 to 98% according to the type of emulsifier used. The results are discussed in relation to published data concerning the problems associated with the use of fish protein in milk replacers.

Description: SummaryThree experiments were carried out to study the effect of replacing milk carbohydrates and part of the milk fat in milk with partially hydrolysed starch (Protamyl 110). Male and female cross-bred lambs (Suffolk × (Finnish Landrace × Dorset Horn)) were used in these experiments. They were weaned from their dams after having received colostrum at 1–2 days of age. The experimental diets were given at room temperature, in eight aliquots, at 3-h intervals through an automated feeding unit. The level of feeding was 1·05 MJ/kg0·75 live weight/day.In the first experiment, the diets were formulated to contain 28, 17 and 55% of their gross energy as protein, carbohydrate and fat respectively. Diet 1 contained glucose, while diets 2, 3 and 4 had 33, 67 and 100% of glucose respectively replaced by Protamyl. There were no significant differences in growth rate and food utilization between levels of starch inclusion.In Expt 2, the maximum Protamyl that could be incorporated in lamb milk replacers was examined. Four experimental diets were prepared so that 17 and 55; 25 and 47; 33 and 39; and 41 and 31% of their gross energy was supplied by Protamyl or butter fat respectively. It was found that up to 33% of the gross energy of the diet (41% of the dry matter) could be replaced with Protamyl without adverse effects on lamb performance. When the level of inclusions was increased to 41% of the gross energy (49% of the dry matter), lamb performance was depressed, particularly during the first 15 days of the experiment.In Expt 3, a direct comparison was made between Protamyl and lactose. The two diets used were similar in composition to diets 1 and 4 in Expt 1, except that lactose was used as a control instead of glucose. There were no differences in growth rate or food utilization as a result of replacing lactose with Protamyl.The results are discussed in relation to the development of carbohydrases in new born ruminants.

Description: SUMMARYSamples of soya-bean meal, groundnut meal, sunflower meal and fish meal were incubated in nylon bags in the rumens of sheep receiving either whole barley or dried grass. For the vegetable sources of protein the rate of disappearance of protein was greater when they were incubated in the rumens of sheep receiving dried grass than in sheep receiving whole barley. The rate of disappearance of fish meal did not vary between sheep fed whole barley or dried grass.Rate of outflow of protein particles from the rumen was determined by rendering the protein supplement totally indigestible by a treatment with sodium dichromate. The rate of outflow was greatest with sheep receiving dried grass and increased with increasing feeding level.The mathematical expressions of degradation rate and outflow rate were combined to give the total amount of protein degraded in the rumen for the grass and barley diets at two levels of feeding.The amino acid composition of residues left in the nylon bags after 9 h of incubation were shown to be almost identical to the amino acid composition of the original protein supplement, indicating that the composition of the amino acid in the undegraded protein entering the abomasum essentially resembled that of the supplements.

Description: SUMMARYIn ruminants, the urinary excretion of purine derivatives (PD) reflects the absorption of microbial purines and can be used as an index of microbial protein supply. The objective of this study, carried out in Aberdeen, 1992, was to examine whether PD concentrations in spot urine or plasma samples vary diurnally during a given feeding regime and if they reflect differences in daily PD excretion induced by varying feed intake. Sixteen sheep were offered ad libitum one of four diets (fresh weight basis, the rest of each diet being minerals and vitamins): (1) 99·9% lucerne (pelleted); (2) 50% hay, 30% barley, 9% fishmeal and 10% molasses; (3) 72% straw, 7% molasses and 20% molassed sugarbeet pulp; and (4) 97% barley. Measurements were made for 1 week after a 2-week adaptation period. Urine was collected daily on days 1–4 and hourly on days 5–7. Hourly urine collection was achieved using a fraction collector. Plasma samples were collected hourly from 09·00 to 17·00 h on day 4. Feed intake varied considerably (347–1718 g DM/day) between diets and between animals. Daily excretion of PD (7·1–22·6 mmol/day) was linearly related to DM intake (r = 0·85, n = 16), and so was the microbial N supply (3·9–19·5 g N/day) estimated from daily PD excretion (r = 0·87). In hourly urine samples, the ratio of PD:creatinine concentrations showed no significant difference between sampling times, and was linearlycorrelated with the daily PD excretion (r = 0·92). Similarly, plasma PD concentration also showed little diurnal fluctuation. Glomerular filtration rate (GFR) increased with feed intake. Plasma PD was not well correlated with daily PD excretion in urine (r = 0·57). The tubular load of PD (plasma PD × GFR) was better correlated with the daily excretion (r = 0·80). It appears that when sheep are fed ad libitum, PD in spot urine may provide a practical indicator of microbial protein supply status.

Description: Field experiments were conducted at the farm of Qinghai Academy of Animal and Veterinary Science, Xining, China during 1996/97 to determine the effects of level of food intake on the urinary excretion of purine derivatives (PD), creatinine and nitrogen in yaks (Bos grunniens). Two experiments were carried out with three female yaks (initial body weight 173–187 kg, age 5 years). For Expt 1 a 3×3 Latin square experimental design was used with three levels of oat hay (nitrogen 13·5 g/kg dry matter (DM)) intake treatments, i.e. 0·3, 0·6 and 0·9 of voluntary intake (1·3–3·5 kg DM/d). Each treatment lasted for 17 days and the samples were collected during the last 7 days of each period. For Expt 2 the animals were fed the same oat hay as in Expt 1 for 3 weeks at a level equivalent to the estimated energy maintenance requirement (M) (1·5–2·2 kg DM/d). The intake was then reduced to 0·6 M on day 1, 0·3 M on day 2 and zero from day 3 until day 10. The animals were re-fed in the reverse order for 3 days. Of the PD, only allantoin and uric acid were present in the urine. The proportions of allantoin and uric acid were 0·86 and 0·14 respectively for both experiments. There was no response of creatinine and nitrogen excretions to feed intake. The rates of PD excretion per kg digestible organic matter (DOM) or digestible dry matter (DDM) were 13·5 and 13·6 mmol respectively. As expected, urinary PD excretion increased significantly (P

Description: SummaryThree experiments were conducted to investigate the influence on nitrogen retention of feeding protein supplements to early weaned lambs direct to the abomasum via the oesophageal groove. In Exp. 1 the lambs received a basal diet of barley and a protein mixture consisting of soyabean meal, sunflower meal and fish meal which was given either in a liquid suspension from a bottle or as dry feed incorporated with the barley. The results showed that the regression coefficients of nitrogen retention on intake were 0.48 and 0.33 with liquid and dry feeding respectively. The regressions of urinary nitrogen on intake were with liquid and dry feeding respectively 0.26 and 0.44.In Exp. 2, various sources of protein were compared isonitrogenously as substitute for part of a barley concentrate ration. The nitrogen intakes and retentions were 14·28 and 5·09, 20·16 and 9·39, 20·53 and 8·19, 19·00 and 7·80, and 18·87 and 7·86 g/day for the control diet and fish meal, yeast, soyabean meal and sunflower meal supplemented diets respectively.In Exp. 3 the fish meal supplement was compared with a high lysine, bloodmeal and with casein as liquid supplements to a basal diet of barley concentrate. The intake and retention of nitrogen were for bloodmeal, casein and fish meal respectively 23·41 and 7·48, 22·56 and 9·11, and 21·88 and 8·31 g/day. It appeared that the protein sources high in methionine resulted in the highest nitrogen retention. The implications of these findings in relation to the nutrition of young ruminants is discussed together with an evaluation of the experimental method as a technique to study postruminal digestion.

Description: SUMMARYThe live-weight gains of male and female lambs were measured during growth from 16 to 40 kg live weight when five cereal-based diets varying in dietary crude protein concentration (from 10 to 20%) were given at three levels in a 5×3×2 factorial design.Males grew faster than females by about 15% (P 〈 0·001); this difference increased with age and with the amount of feed given (P 〈 0·05). Growth rate responded linearly to increase in feeding level (P 〈 0·001) and curvilinearly to increase in dietary protein concentration (P 〈 0·01). A significant interaction (P 〈 0·01) occurred whereby growth increased with higher protein concentrations as feeding level increased.At the highest feeding level (near ad libitum) the results suggest that the optimum dietary crude protein concentration for growth was about 17·5, 15·0, 12·5 and 12·5% at body weights of 20, 25, 30 and 35 kg respectively. The overall optimum dietary crude protein concentration for growth between 16 and 40 kg body weight was about 17·0, 15·0 and 11·0% when the mean digestible energy intake was 3·0, 2·6 and 2·1 Mcal/day.The results agree reasonably well with estimates of protein requirements for lambs given by the Agricultural Research Council (1965).

Description: SUMMARY1. In an experiment with ninety-nine lambs the effects on the body composition of male and female lambs were examined when five diets containing different concentrations of crude protein (in the range 10–20%) were given at three levels of feeding and lambs were slaughtered at two live weights (27·5 and 40 kg).2. With lambs slaughtered at 27·5 kg there were significant increases in the rate of both nitrogen and fat retention with increases in levels of feeding. There were also linear increases in the rate of protein deposition and decreases in fat deposition with increases in the concentration of crude protein. This effect was particularly marked at the high level of feeding.3. With lambs slaughtered at 40 kg live weight there were also linear increases in fat and in nitrogen deposition with increasing feeding level but the effect of increasing the protein concentration on increases in nitrogen retention departed from linearity.4. While at 27·5 kg there were no significant effects of feeding level on nitrogen and ether-extract content of the bodies at slaughter, with animals slaughtered at 40 kg there was a significant linear decrease in ether-extract content with increasing feeding level and a corresponding linear increase in nitrogen content with increased level of feeding.5. Male lambs deposited more nitrogen and less fat than females. This was true of both rate of deposition and of carcass composition at 40 kg live weight.