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  • 1
    Digitale Medien
    Digitale Medien
    Deformation, high-pressure metamorphism and exhumation of ultramafic rocks in a deep subduction/collision setting (Cabo Ortegal, NW Spain) (1999)
    Oxford, UK : Blackwell Science Inc
    Journal of metamorphic geology 17 (1999), S. 0 
    Details
    ISSN: 1525-1314
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Geologie und Paläontologie
    Notizen: New petrological and microstructural data from various metaperidotite ‘boudins’ within large ductile shear zones in the Cabo Ortegal allochthonous complex in NW Spain have important implications for the tectonic models of the area. The peridotites (mylonitic garnet harzburgite, Ti-clinohumite and magnesite–olivine orthopyroxenite) contain mineral assemblages that equilibrated at high- to ultra-high-pressure metamorphic conditions as well as microstructures of tectonic origin formed at temperatures well above 800 °C. Olivine and orthopyroxene fabrics resulted from flow at high temperature (〉1000 °C) and solid-state non-coaxial plastic flow at intermediate temperature (800–900 °C). Flow caused dynamic recrystallization and formation of moderate to strong lattice preferred orientations under low to moderate differential stresses and strain rates characteristic of upper mantle and deep crustal deformation. The microstructures and textural relationships suggest that the mylonitic garnet harzburgite represents mantle fragments with lithospheric and asthenospheric imprints, whereas the olivine orthopyroxenite resulted from serpentinite burial to depths where it acquired a characteristic high/ultra-high-pressure metamorphic signature. Both types of ultramafites converged to a common site in a subduction zone that was later incorporated during continental collision to the NW Iberian Massif as exotic, allochthonous complexes that record structural and metamorphic evidence of the earliest phases of the Hercynian orogeny.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1525-1314.1999.00227.x
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  • 2
    Digitale Medien
    Digitale Medien
    Single mutations of residues outside the active center of the xylanase Xys1Δ from Streptomyces halstedii JM8 affect its activity (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 240 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Mutagenesis of the xylanase Xys1 of Streptomyces halstedii JM8 has been done by error prone PCR. Mutants with modified hydrolytic activity were isolated, the recombinant variant proteins purified and the catalytic activities of each one determined and compared with the wild type enzyme. Two of the isolated single point mutants, m1 (G133D) and m8 (N148D), showed 22–25% increase in specific activity towards xylan compared to wild type xylanase. Two other mutants, m5a (D175A) and m7 (T160A), showed a significant reduction in specific activity of 40–50% with respect to the wild type enzyme. These residues are mainly located in the βα-loops of the xylanase, the region showing the main structural divergences within family 10 of xylanases. This study shows the usefulness of random mutagenesis to point out some key residues not directly involved in the active center, but in which mutation produces subtle structural rearrangements affecting the enzymatic function.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/j.femsle.2004.09.032
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  • 3
    Digitale Medien
    Digitale Medien
    Plant-adapted green fluorescent protein is a versatile vital reporter for gene expression, protein localization and mitosis in the filamentous fungus, Aspergillus nidulans (1998)
    Oxford BSL : Blackwell Publishing Ltd
    Molecular microbiology 27 (1998), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Green fluorescent protein (GFP) is a useful reporter to follow the in vivo behaviour of proteins, but the wild-type gfp gene does not function in many organisms, including many plants and filamentous fungi. We show that codon-modified forms of gfp, produced for use in plants, function effectively in Aspergillus nidulans both as gene expression reporters and as vital reporters for protein location. To demonstrate the use of these modified gfps as reporter genes we have used fluorescence to follow ethanol-induced GFP expression from the alcA promoter. Translational fusions with the modified gfp were used to follow protein location in living cells; plant ER-retention signals targeted GFP to the endoplasmic reticulum, whereas fusion to the GAL4 DNA-binding domain targeted it to the nucleus. Nuclear-targeted GFP allowed real-time observation of nuclear movement and division. These modified gfp genes should provide useful markers to follow gene expression, organelle behaviour and protein trafficking in real time.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1365-2958.1998.00664.x
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  • 4
    Digitale Medien
    Digitale Medien
    Dynamic distribution of BIMGPP1 in living hyphae of Aspergillus indicates a novel role in septum formation (2002)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 45 (2002), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Mutation of bimG, the major protein phosphatase 1 gene in Aspergillus nidulans, causes multiple cell cycle and hyphal growth defects that are associated with overphosphorylation of subcellular components. We have used functional translational fusions with the green fluorescent protein (GFP) to show that BIMG has at least four discrete locations within growing hyphae. Three of these locations, the hyphal tip, the spindle pole body and the nucleus, correlate with previously known requirements for bimGPP1 in mitosis and hyphal growth and are highly dynamic. BIMG-GFP in the hyphal tip seemed to be associated with the plasma membrane and formed a collar of fluorescence within the apical dome. The distribution of nuclear BIMG-GFP varied depending on nutritional conditions; on poor medium, it concentrated more in the nucleolus than in the nucleoplasm, whereas on rich medium, it was more evenly distributed between the two nuclear regions. The association of BIMG-GFP with developing septa was transient, and we present evidence that BIMG phosphatase plays a direct role in septum formation, distinct from its role in mitosis. We conclude that, by being physically present at several sites, the BIMG phosphatase has roles in multiple cellular processes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1365-2958.2002.03092.x
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  • 5
    Digitale Medien
    Digitale Medien
    The Philippine cynomolgus monkey ( Macaca fasicularis ) provides a new nonhuman primate model of tuberculosis that resembles human disease (1996)
    [s.l.] : Nature Publishing Group
    Nature medicine 2 (1996), S. 430-436 
    Details
    ISSN: 1546-170X
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Medizin
    Notizen: [Auszug] A nonhuman primate model of tuberculosis that closely resembles human disease is urgently needed. We have evaluated the Philippine cynomolgus monkey, Macaca fasicularis, as a model of TB. Cynomolgus monkeys challenged intratracheally with extremely high doses of Mycobacterium tuberculosis (105 or ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/nm0496-430
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  • 6
    Digitale Medien
    Digitale Medien
    Effect of carbon source on the expression of celA1, a cellulase-encoding gene from Streptomyces halstedii JM8 (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 153 (1997), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: The production of the cellulase Cel1 from Streptomyces halstedii JM8 was studied in cells grown in the presence of glucose, cellobiose or microcrystalline cellulose (Avicel). Among these, glucose repressed expression while cellobiose and Avicel exerted a clear induction. The gene celA1 was cloned in several heterologous Streptomyces hosts and its expression analyzed. S. parvulus transformed with the plasmid pJM11, a pIJ702 derivative, was the best producer. A region which includes the sequence ATTGGGACCGCTTCC located between positions −161 and −147 upstream from the translation initiation codon [Fernández-Abalos et al. (1992) J. Bacteriol. 174, 6368–6376] was deleted and its effect was studied in the presence of different carbon sources. Although the observed effect depends on the host used, this region seems to be involved in activation of the expression of this gene.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1997.tb10469.x
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  • 7
    Digitale Medien
    Digitale Medien
    TUNEL and limited immunophenotypic analyses of apoptosis in paucibacillary and multibacillary leprosy lesions (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 41 (2004), S. 0 
    Details
    ISSN: 1574-695X
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Some mycobacterial infections, such as tuberculosis, are characterized by apoptosis of infected or by-stander mononuclear immune cells. For localized (paucibacillary, PB) and disseminated (multibacillary, MB) leprosy, characterized by polarized Th1-like vs. Th2-like immune responses, respectively, little is known about lesional apoptosis. We analyzed sections of paraffin-embedded, untreated leprosy lesions from 21 patients by an indirect immunofluorescent terminal deoxynucleotide-transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay. Some TUNEL (+) PB sections were then reacted with phycoerythrin-conjugated (red) antibodies against T cells, monocytes, or antigen-presenting (Langerhans) cells. TUNEL (+) bodies were detected in 9 of 16 PB lesions (56%) and in 1 of 5 MB lesions (20%). Some TUNEL (+) bodies in PB disease were CD3+ (T cell), as well as CD4+ (T-helper) or CD8+ (T-cytotoxic). Apoptosis characterizes PB and MB leprosy lesions and may be more frequent in PB disease. In PB disease, some TUNEL (+) bodies may derive from T cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/j.femsim.2004.04.002
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  • 8
    Digitale Medien
    Digitale Medien
    Biosorption of Heavy Metals by 〈i〉Pseudomonas aeruginosa〈/i〉 Isolated from a Petroleum Contaminated Site (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 20-21 (July 2007), p. 615-618 
    Details
    ISSN: 1662-8985
    Quelle: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Thema: Maschinenbau
    Notizen: The study describes the sorption of Cr, Cu, Mn and Zn by Pseudomonas aeruginosaAT18 isolated from a site contaminated with petroleum and heavy metals. The concentrationsstudied (mgL-1) were Cr-50, Cu-49, Mn-60 and Zn-70. The solution pH and ionic strength werevery important factors in the metal biosorption performance and the biosorption capacity ofPseudomonas aeruginosa AT18 for Cr3+ Cu2+, Mn2+ and Zn2+. In aqueous solution the biosorptionincreased with increasing pH in the range 5.46-7.72. The results obtained in the experimental assaysshow that Pseudomonas aeruginosa AT18 has the capacity for biosorption of the metallic ions Cr3+,Cu2+ and Zn2+ in solutions, although its capacity for the sorption of manganese is low (22.39 mgMn2+/g of biomass) in comparison to the Cr3+, Cu2+ and Zn2+ ions, as shown by the individualanalyses. However, 20% of the manganese was removed from an initial concentration of 49.0 mgL-1, with a Qm value similar to that obtained in solutions containing mixtures of Cr3+ Cu2+, Mn2+ andZn2+. The chromium level sorbed by Pseudomonas aeruginosa AT18 biomass was higher than thatfor Cu, Mn and Zn, with 100% removal in the pH range 7.00-7.72 and a Qm of 121.90-200.00 mgof Cr3+/g of biomass. The remove of Cr, Cu and Zn are a result also of precipitation processes
    Materialart: Digitale Medien
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.20-21.615.pdf
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  • 9
    Digitale Medien
    Digitale Medien
    Milkfish seedling survival in raceways of freshwater recirculating systems
    Amsterdam : Elsevier
    Aquaculture 36 (1984), S. 257-272 
    Details
    ISSN: 0044-8486
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0044-8486(84)90241-2
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  • 10
    Digitale Medien
    Digitale Medien
    Effects of complexing agents and acid modifiers on the supercritical fluid extraction of native phenyl- and butyl-tins from sediment (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Applied Organometallic Chemistry 12 (1998), S. 577-584 
    Details
    ISSN: 0268-2605
    Schlagwort(e): organotin ; extraction ; analysis ; GC-FPD ; Chemistry ; Industrial Chemistry and Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: The effects of: (1) the modifier type (methanol and formic, acetic and propionic acids), (2) complexing agents (diethylammonium diethyldithiocarbamate, ammonium pyrrolidinedithiocarbamate) either with or without acidic modifiers, (3) the extraction temperature (50-80 °C) and pressure (30-50 MPa), (4) the extraction procedure (static-dynamic or dynamic), and (5) the volume of static modifier, on the extraction efficiency of native butyl- and phenyl-tin compounds from sediment, were evaluated comprehensively. The highest extraction efficiency for butyl- and phenyl-tin compounds was obtained at 30 MPa and 50 °C by using CO2 modified with acetic acid (200 μl in the cell). Supercritical fluid extraction (SFE) extracts were hexylated and determined by GC-FPD using a 610 nm bandpass filter without any clean-up step. In summary, the developed analytical procedure is robust (no restrictor clogging; free from FPD interferences), it is low-cost (no complexing agents needed), it has a high sample throughput (〈3 h), it is independent of the matrix for the determination of butyltin compounds in sediment, and it provides the highest precision among the SFE procedures reported for organotin determination. © 1998 John Wiley & Sons, Ltd.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
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