Publication Date:
1995-02-24
Description:
The radiosensitive rodent mutant cell line xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive human malignant glioma M059J cell line was found to be defective in DNA double-strand break repair, but fails to express the p350 subunit of DNA-PK. These results suggest that DNA-PK kinase activity is involved in DNA double-strand break repair.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lees-Miller, S P -- Godbout, R -- Chan, D W -- Weinfeld, M -- Day, R S 3rd -- Barron, G M -- Allalunis-Turner, J -- New York, N.Y. -- Science. 1995 Feb 24;267(5201):1183-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biological Science, University of Calgary, Alberta, Canada.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7855602" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
*Antigens, Nuclear
;
CHO Cells
;
Cell Line, Transformed
;
Cricetinae
;
DNA/metabolism
;
*DNA Helicases
;
*DNA Repair
;
DNA-Activated Protein Kinase
;
DNA-Binding Proteins/analysis
;
Gamma Rays
;
Humans
;
Molecular Sequence Data
;
Nuclear Proteins/analysis
;
Protein-Serine-Threonine Kinases/*metabolism
;
*Radiation Tolerance
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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