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Effect of complex nitrogen source on the synthesis and accumulation of poly(3-hydroxybutyric acid) by recombinantEscherichia coli in flask and fed-batch cultures (1994)

Lee, Sang Yup ; Chang, Ho Nam

Springer

Journal of polymers and the environment 2 (1994), S. 169-176

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ISSN: 1572-8900

Keywords: Escherichia coli ; poly(3-hydroxybutyric acid) ; defined medium ; complex nitrogen source ; fed-batch culture

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: Abstract When a recombinantEscherichia coli XL1-Blue harboring pSYL105 was cultured in a complex medium, a poly(3-hydroxybutyric acid) (PHB) concentration of 7.16 g/L was obtained in 48 h. However, a PHB concentration of only 0.91 g/L was obtained in 60 h by culturing in a defined medium. Also, fed-batch culture in a defined medium resulted in considerably lower PHB accumulation than in a complex medium. With the aim to produce a high concentration of PHB at a reduced medium cost, we examined 10 complex nitrogen sources for their ability to promote PHB synthesis in a defined medium. Tryptone, casamino acids, and casein hydrolysate promoted PHB synthesis to a higher extent than the others tested. PHB synthesis was also enhanced during fedbatch cultures when a defined medium was supplemented with various complex nitrogen sources. With tryptone supplementation a PHB concentration of 66.7 g/L could be obtained in 44 h. Yeast extract was less effective for promoting PHB synthesis than tryptone. Corn steep liquor, which did not enhance PHB synthesis significantly, could promote PHB synthesis considerably when supplemented together with yeast extract in both flask and fed-batch cultures.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02067442

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Production of poly(3-hydroxybutyric acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control (1994)

Kim, Beom Soo ; Lee, Seung Chul ; Lee, Sang Yup ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 43 (1994), S. 892-898

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ISSN: 0006-3592

Keywords: poly(3-hydroxybutyric acid) ; high cell density culture ; Alcaligenes eutrophus ; on-line glucose analyzer ; mass spectrometer ; fed-batch culture ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Alcaligenes eutrophus NCIMB 11599 was cultivated to produce poly(3-hydroxybutyric acid) (PHB) from glucose by the automatic fed-batch culture technique. The glucose concentration of the culture broth was controlled at 10 to 20 g/L by two methods: using exit gas data obtained from a mass spectrometer and using an on-line glucose analyzer. The effect of ammonium limitation on PHB synthesis at different culture phases was studied. The final cell concentration, PHB concentration, and PHB productivity increased as ammonia feeding was stopped at a higher cell concentration. High concentrations of PHB (121 g/L) and total cells (164 g/L) were obtained in 50 h when ammonia feeding was stopped at the cell concentration of 70 g/L. The maximum PHB content reached 76% of dry cell weight and the productivity was 2.42 g/L h with the yield of 0.3 g PHB/g glucose.

Additional Material: 5 Ill.